Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Feb. 7, 2018

The simultaneous imaging and manipulating of neural activity could enable the functional dissection circuits. Here we have combined two-photon optogenetics with volumetric calcium to measure manipulate in mouse neocortex vivo three-dimensions (3D) cellular resolution. Using a hybrid holographic approach, simultaneously photostimulate more than 80 neurons over 150 μm depth layer 2/3 visual cortex, while surrounding neurons. We validate usefulness method by photoactivating 3D selected groups interneurons, suppressing response nearby pyramidal stimuli awake animals. Our all-optical approach be used as general platform read write neuronal activity.

Language: Английский

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Interacting neural ensembles in orbitofrontal cortex for social and feeding behaviour

Nature, Journal Year: 2019, Volume and Issue: 565(7741), P. 645 - 649

Published: Jan. 1, 2019

Language: Английский

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Adult Hippocampal Neurogenesis, Fear Generalization, and Stress

Neuropsychopharmacology, Journal Year: 2015, Volume and Issue: 41(1), P. 24 - 44

Published: June 12, 2015

Language: Английский

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A Systems Neuroscience Approach to Migraine

Neuron, Journal Year: 2018, Volume and Issue: 97(5), P. 1004 - 1021

Published: March 1, 2018

Language: Английский

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Attractor and integrator networks in the brain

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(12), P. 744 - 766

Published: Nov. 3, 2022

Language: Английский

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