Neuronal
ensembles,
coactive
groups
of
neurons
found
in
spontaneous
and
evoked
cortical
activity,
are
causally
related
to
memories
perception,
but
it
is
still
unknown
how
stable
or
flexible
they
over
time.
We
used
two-photon
multiplane
calcium
imaging
track
weeks
the
activity
same
pyramidal
layer
2/3
visual
cortex
from
awake
mice
recorded
their
visually
responses.
Less
than
half
remained
active
across
any
two
sessions.
These
formed
ensembles
that
lasted
weeks,
some
were
also
transient
appeared
only
one
single
session.
Stable
preserved
most
for
up
46
days,
our
longest
imaged
period,
these
'core'
cells
had
stronger
functional
connectivity.
Our
results
demonstrate
neuronal
can
last
could,
principle,
serve
as
a
substrate
long-lasting
representation
perceptual
states
memories.
Science,
Journal Year:
2019,
Volume and Issue:
365(6453)
Published: July 18, 2019
Perceptual
experiences
may
arise
from
neuronal
activity
patterns
in
mammalian
neocortex.
We
probed
mouse
neocortex
during
visual
discrimination
using
a
red-shifted
channelrhodopsin
(ChRmine,
discovered
through
structure-guided
genome
mining)
alongside
multiplexed
multiphoton-holography
(MultiSLM),
achieving
control
of
individually
specified
neurons
spanning
large
cortical
volumes
with
millisecond
precision.
Stimulating
critical
number
stimulus-orientation-selective
drove
widespread
recruitment
functionally
related
neurons,
process
enhanced
by
(but
not
requiring)
orientation-discrimination
task
learning.
Optogenetic
targeting
orientation-selective
ensembles
elicited
correct
behavioral
discrimination.
Cortical
layer-specific
dynamics
were
apparent,
as
emergent
asymmetrically
propagated
layer
2/3
to
5,
and
smaller
5
effective
larger
eliciting
orientation
behavior.
Population
emerging
after
optogenetic
stimulation
both
correctly
predicted
behavior
resembled
natural
internal
representations
stimuli
at
cellular
resolution
over
cortex.
Annual Review of Neuroscience,
Journal Year:
2020,
Volume and Issue:
43(1), P. 249 - 275
Published: July 8, 2020
Significant
experimental,
computational,
and
theoretical
work
has
identified
rich
structure
within
the
coordinated
activity
of
interconnected
neural
populations.
An
emerging
challenge
now
is
to
uncover
nature
associated
computations,
how
they
are
implemented,
what
role
play
in
driving
behavior.
We
term
this
computation
through
population
dynamics.
If
successful,
framework
will
reveal
general
motifs
quantitatively
describe
dynamics
implement
computations
necessary
for
goal-directed
Here,
we
start
with
a
mathematical
primer
on
dynamical
systems
theory
analytical
tools
apply
perspective
experimental
data.
Next,
highlight
some
recent
discoveries
resulting
from
successful
application
systems.
focus
studies
spanning
motor
control,
timing,
decision-making,
working
memory.
Finally,
briefly
discuss
promising
lines
investigation
future
directions
framework.
Cell,
Journal Year:
2020,
Volume and Issue:
183(6), P. 1586 - 1599.e10
Published: Nov. 6, 2020
The
hippocampus
is
crucial
for
spatial
navigation
and
episodic
memory
formation.
Hippocampal
place
cells
exhibit
spatially
selective
activity
within
an
environment
have
been
proposed
to
form
the
neural
basis
of
a
cognitive
map
space
that
supports
these
mnemonic
functions.
However,
direct
influence
cell
on
behavior
has
not
yet
demonstrated.
Using
'all-optical'
combination
simultaneous
two-photon
calcium
imaging
optogenetics,
we
identified
selectively
activated
encoded
behaviorally
relevant
locations
in
virtual
reality
environment.
Targeted
stimulation
small
number
was
sufficient
bias
animals
during
task,
providing
causal
evidence
hippocampal
actively
support
memory.
Nature Communications,
Journal Year:
2021,
Volume and Issue:
12(1)
Published: Jan. 21, 2021
Investigation
of
neural
circuit
dynamics
is
crucial
for
deciphering
the
functional
connections
among
regions
brain
and
understanding
mechanism
dysfunction.
Despite
advancements
models
in
vitro,
technologies
both
precisely
monitoring
modulating
activities
within
three-dimensional
(3D)
have
yet
to
be
developed.
Specifically,
no
existing
3D
microelectrode
arrays
(MEAs)
integrated
capabilities
stimulate
surrounding
neurons
monitor
temporal
evolution
formation
a
network
real
time.
Herein,
we
present
high-density
multifunctional
MEA
with
optical
stimulation
drug
delivery
investigating
engineered
tissues.
We
demonstrate
precise
measurements
synaptic
latencies
networks.
expect
our
open
up
opportunities
studies
circuits
through
precise,
vitro
investigations
models.
Cell Reports,
Journal Year:
2019,
Volume and Issue:
26(3), P. 802 - 813.e4
Published: Jan. 1, 2019
Imaging
voltage
using
fluorescent-based
sensors
could
be
an
ideal
technique
to
probe
neural
circuits
with
high
spatiotemporal
resolution.
However,
due
insufficient
signal-to-noise
ratio
(SNR),
imaging
membrane
potential
in
mammalian
preparations
is
still
challenging.
In
recent
years,
many
genetically
encoded
indicators
(GEVIs)
have
been
developed.
To
compare
them
and
guide
decisions
on
which
GEVI
use,
we
characterized
side
by
the
performance
of
eight
GEVIs
that
represent
different
families
molecular
constructs.
We
tested
vitro
1-photon
vivo
wide-field
2-photon
imaging.
find
QuasAr2
exhibited
best
vitro,
whereas
only
ArcLight-MT
used
reliably
detect
electrical
activity
excitation.
No
single
was
for
every
experiment.
These
results
provide
a
choosing
optimal
specific
applications.
