Modulation of Drosophila post-feeding physiology and behavior by the neuropeptide leucokinin DOI Creative Commons
Meet Zandawala, Maria E. Yurgel, Michael J. Texada

et al.

PLoS Genetics, Journal Year: 2018, Volume and Issue: 14(11), P. e1007767 - e1007767

Published: Nov. 20, 2018

Behavior and physiology are orchestrated by neuropeptides acting as central neuromodulators circulating hormones. An outstanding question is how these function to coordinate complex competing behaviors. In Drosophila, the neuropeptide leucokinin (LK) modulates diverse functions, but mechanisms underlying interactions remain poorly understood. As a first step towards understanding mechanisms, we delineated LK circuitry that governs various aspects of post-feeding behavior. We found impaired signaling in Lk receptor (Lkr) mutants affects coordinated processes, including regulation stress, water homeostasis, feeding, locomotor activity, metabolic rate. Next, sought define populations neurons contribute different this physiology. find calcium activity abdominal ganglia (ABLKs), not two sets brain neurons, increases specifically following consumption, suggesting ABLKs regulate homeostasis its associated To identify targets peptide, mapped distribution Lkr expression, mined single-cell transcriptome dataset for genes coexpressed with Lkr, identified synaptic partners neurons. expression insulin-producing cells (IPCs), gut, renal tubules chemosensory cells, correlates well regulatory roles detected mutants. Furthermore, flies targeted knockdown IPCs displayed altered insulin-like peptides (DILPs) transcripts increased starvation resistance. Thus, some effects appear occur via DILP action. Collectively, our data suggest three have targets, modulate establishment post-prandial regulating distinct physiological processes behaviors such diuresis, metabolism, organismal insulin signaling. These findings provide platform investigating feeding-related neuroendocrine vital behavior

Language: Английский

A connectome and analysis of the adult Drosophila central brain DOI Creative Commons
Louis K. Scheffer, C. Shan Xu, Michał Januszewski

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Sept. 3, 2020

The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction brain fruit fly Drosophila melanogaster . Improved include procedures to prepare, image, align, segment, find synapses in, proofread such data sets. define cell types, refine computational compartments, provide an exhaustive atlas examples many them novel. detailed consisting neurons their chemical most central brain. make public simplify access, reducing effort needed answer circuit questions, linking defined by our analysis with genetic reagents. Biologically, we examine distributions connection strengths, motifs on different scales, electrical consequences compartmentalization, evidence that maximizing packing density is important criterion in evolution fly’s

Language: Английский

Citations

988
Genetic Dissection of Neural Circuits: A Decade of Progress DOI Creative Commons
Liqun Luo, Edward M. Callaway, Karel Svoboda

et al.

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

Citations

449
Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior DOI
Dick R. Nässel, Meet Zandawala

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

Citations

307
A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection DOI Creative Commons
Brad K. Hulse, Hannah Haberkern, Romain Franconville

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Oct. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Language: Английский

Citations

304
Split-TurboID enables contact-dependent proximity labeling in cells DOI Open Access
Kelvin F. Cho, Tess C. Branon, Sanjana Rajeev

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2020, Volume and Issue: 117(22), P. 12143 - 12154

Published: May 18, 2020

Significance Most of the thousands proteins that comprise a human cell have specific subcellular localization patterns essential for their function. “Proximity labeling” (PL) is method mapping endogenous cellular on proteome-wide scale. To improve specificity and versatility PL, we developed split-TurboID, promiscuous biotinylating enzyme split into two inactive fragments. The fragments are coexpressed in cells brought together by drug, protein–protein interaction, or organelle contact to reconstitute TurboID enzymatic activity. We used split-TurboID map protein composition endoplasmic reticulum–mitochondria sites, which mitochondrial fission, lipid biosynthesis, calcium signaling. For conditional higher-specificity may be valuable tool biological discovery.

Language: Английский

Citations

249
Architectures of neuronal circuits DOI
Liqun Luo

Science, Journal Year: 2021, Volume and Issue: 373(6559)

Published: Sept. 2, 2021

Although individual neurons are the basic unit of nervous system, they process information by working together in neuronal circuits with specific patterns synaptic connectivity. Here, I review common circuit motifs and architectural plans used diverse brain regions animal species. also consider how these architectures assemble during development might have evolved. Understanding connectivity can implement neural computations will help to bridge huge gap between biology neuron function entire brain, allow us better understand basis behavior, may inspire new advances artificial intelligence.

Language: Английский

Citations

229
A Circadian Output Circuit Controls Sleep-Wake Arousal in Drosophila DOI Creative Commons
Fang Guo, Meghana Holla, Madelen M. Díaz

et al.

Neuron, Journal Year: 2018, Volume and Issue: 100(3), P. 624 - 635.e4

Published: Sept. 27, 2018

Language: Английский

Citations

173
Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body DOI Creative Commons
Michael-John Dolan, Shahar Frechter, Alexander Shakeel Bates

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: May 21, 2019

Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher centre, the lateral horn (LH), is implicated in behaviour. However, our structural and functional understanding LH scant, large part due lack sparse neurogenetic tools for this region. We generate collection split-GAL4 driver lines providing genetic access 82 cell types. use these create an anatomical neurotransmitter map link EM connectomics data. find ~30% projections converge with outputs from mushroom body, site learning memory. Using optogenetic activation, we identify types that drive changes valence behavior or specific locomotor programs. summary, have generated resource manipulating mapping neurons, new insights into circuit basis learned behavior.

Language: Английский

Citations

168
Algorithms for Olfactory Search across Species DOI Creative Commons
Keeley L. Baker, Michael H. Dickinson, Teresa M Findley

et al.

Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 38(44), P. 9383 - 9389

Published: Oct. 31, 2018

Localizing the sources of stimuli is essential. Most organisms cannot eat, mate, or escape without knowing where relevant originate. For many, if not most, animals, olfaction plays an essential role in search. While microorganismal chemotaxis relatively well understood, larger animals algorithms and mechanisms olfactory search remain mysterious. In this symposium, we will present recent advances our understanding flies rodents. Despite their different sizes behaviors, both species must solve similar problems, including meeting challenges turbulent airflow, sampling environment to optimize information, incorporating odor information into broader navigational systems.

Language: Английский

Citations

163
A Connectome of the AdultDrosophilaCentral Brain DOI Creative Commons
C. Shan Xu, Michał Januszewski, Zhiyuan Lu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Jan. 21, 2020

Abstract The neural circuits responsible for behavior remain largely unknown. Previous efforts have reconstructed the complete of small animals, with hundreds neurons, and selected larger animals. Here we (the FlyEM project at Janelia collaborators Google) summarize new methods present circuitry a large fraction brain much more complex animal, fruit fly Drosophila melanogaster . Improved include procedures to prepare, image, align, segment, find synapses, proofread such data sets; that define cell types based on connectivity in addition morphology; simplify access evolving set. From resulting derive better definition computational compartments their connections; an exhaustive atlas examples types, many them novel; detailed most central brain; exploration statistics structure different compartments, as whole. We make public, web site resources specifically designed it easy explore, all levels expertise from expert merely curious. public availability these data, simplified means it, dramatically reduces effort needed answer typical circuit questions, identity upstream downstream partners, regions, link neurons defined by our analysis genetic reagents can be used study functions. Note: In next few weeks, will release series papers involved discussions. One paper detail hemibrain reconstruction extensive interpretation made possible this dense connectome. Another explore complex, region navigation, motor control, sleep. A final insights mushroom body, center multimodal associative learning brain.

Language: Английский

Citations

145