Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: March 22, 2022
Abstract
Reconstructing
axonal
projections
of
single
neurons
at
the
whole-brain
level
is
currently
a
converging
goal
neuroscience
community
that
fundamental
for
understanding
logic
information
flow
in
brain.
Thousands
from
different
brain
regions
have
recently
been
morphologically
reconstructed,
but
corresponding
physiological
functional
features
these
reconstructed
are
unclear.
By
combining
two-photon
Ca
2+
imaging
with
targeted
single-cell
plasmid
electroporation,
we
reconstruct
brain-wide
morphologies
defined
by
sound-evoked
response
map
auditory
cortices
(AUDs)
awake
mice.
Long-range
interhemispheric
can
be
reliably
labelled
via
co-injection
an
adeno-associated
virus,
which
enables
enhanced
expression
indicator
protein
neurons.
Here
show
this
method
avoids
randomness
and
ambiguity
conventional
methods
neuronal
morphological
reconstruction,
offering
avenue
developing
precise
one-to-one
projection
patterns
features.
Cerebral Cortex,
Journal Year:
2019,
Volume and Issue:
30(3), P. 1603 - 1622
Published: Aug. 28, 2019
Abstract
The
mouse
auditory
cortex
(ACtx)
contains
two
core
fields—primary
(A1)
and
anterior
field
(AAF)—arranged
in
a
mirror
reversal
tonotopic
gradient.
best
frequency
(BF)
organization
naming
scheme
for
additional
higher
order
fields
remain
matter
of
debate,
as
does
the
correspondence
between
smoothly
varying
global
tonotopy
heterogeneity
local
cellular
tuning.
Here,
we
performed
chronic
widefield
two-photon
calcium
imaging
from
ACtx
awake
Thy1-GCaMP6s
reporter
mice.
Data-driven
parcellation
maps
identified
five
fields,
including
previously
unidentified
area
at
ventral
posterior
extreme
(VPAF)
tonotopically
organized
suprarhinal
(SRAF)
that
extended
laterally
far
ectorhinal
cortex.
Widefield
were
stable
over
time,
where
single
pixel
BFs
fluctuated
by
less
than
0.5
octaves
throughout
1-month
period.
After
accounting
neuropil
signal
tuning
strength,
BF
neighboring
layer
2/3
neurons
was
intermediate
to
heterogeneous
salt
pepper
highly
precise
have
each
been
described
prior
studies.
Multiscale
data
suggest
there
is
no
ultrasonic
or
secondary
mouse.
Instead,
VPAF
dorsal
(DP)
emerged
strongest
candidates
areas.
Nature Communications,
Journal Year:
2018,
Volume and Issue:
9(1)
Published: Nov. 29, 2018
The
basolateral
amygdala
(BLA)
plays
important
roles
in
associative
learning,
by
representing
conditioned
stimuli
(CSs)
and
unconditioned
(USs),
forming
associations
between
CSs
USs.
However,
how
such
are
formed
updated
remains
unclear.
Here
we
show
that
learning
driven
reward
punishment
profoundly
alters
BLA
population
responses,
reducing
noise
correlations
transforming
the
representations
of
to
resemble
valence-specific
This
transformation
is
accompanied
emergence
prevalent
inhibitory
CS
US
plasticity
responses
individual
neurons.
During
reversal
wherein
expected
valences
reversed,
remapped
onto
ensembles
opposite
predict
switching
behaviors.
Our
results
reveal
signals
predictive
opposing
evolve
during
these
thereby
guiding
flexible
Proceedings of the National Academy of Sciences,
Journal Year:
2018,
Volume and Issue:
115(17)
Published: April 9, 2018
Quantifying
the
functional
relations
between
nodes
in
a
network
based
on
local
observations
is
key
challenge
studying
complex
systems.
Most
existing
time
series
analysis
techniques
for
this
purpose
provide
static
estimates
of
properties,
pertain
to
stationary
Gaussian
data,
or
do
not
take
into
account
ubiquitous
sparsity
underlying
networks.
When
applied
spike
recordings
from
neuronal
ensembles
undergoing
rapid
task-dependent
dynamics,
they
thus
hinder
precise
statistical
characterization
dynamic
networks
adaptive
behavior.
We
develop
estimation
and
inference
paradigm
extracting
dynamics
sense
Granger,
by
integrating
filtering,
compressed
sensing,
point
process
theory,
high-dimensional
statistics.
demonstrate
utility
our
proposed
through
theoretical
analysis,
algorithm
development,
application
synthetic
real
data.
Application
two-photon
Ca2+
imaging
experiments
mouse
auditory
cortex
reveals
unique
features
structures
spontaneous
activity
at
unprecedented
spatiotemporal
resolution.
Our
simultaneous
ferret
prefrontal
cortical
areas
suggests
evidence
role
top-down
bottom-up
across
these
involved
robust
attentive
Neuron,
Journal Year:
2019,
Volume and Issue:
104(6), P. 1168 - 1179.e5
Published: Nov. 11, 2019
Driving
perception
by
direct
activation
of
neural
ensembles
in
cortex
is
a
necessary
step
for
achieving
causal
understanding
the
code
auditory
and
developing
central
sensory
rehabilitation
methods.
Here,
using
optogenetic
manipulations
during
an
discrimination
task
mice,
we
show
that
can
be
short-circuited
coarser
pathways
simple
sound
identification.
Yet
when
decision
becomes
more
complex,
involving
temporal
integration
information,
activity
required
targeted
specific
cortical
changes
perceptual
decisions,
as
predicted
our
readout
code.
Hence,
representations
contribute
to
discriminations
refining
decisions
from
parallel
routes.
