Coupling between fast and slow oscillator circuits in Cancer borealis is temperature-compensated

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Feb. 4, 2021

Coupled oscillatory circuits are ubiquitous in nervous systems. Given that most biological processes temperature-sensitive, it is remarkable the neuronal of poikilothermic animals can maintain coupling across a wide range temperatures. Within stomatogastric ganglion (STG) crab, Cancer borealis , fast pyloric rhythm (~1 Hz) and slow gastric mill (~0.1 precisely coordinated at ~11°C such there an integer number cycles per cycle (integer coupling). Upon increasing temperature from 7°C to 23°C, both oscillators showed similar temperature-dependent increases frequency, between was conserved. Thus, although rhythms show changes couple these their coordination over Such robustness could be part toolbox enables neural function despite global perturbations.

Language: Английский

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From the Neuroscience of Individual Variability to Climate Change

Journal of Neuroscience, Journal Year: 2021, Volume and Issue: 41(50), P. 10213 - 10221

Published: Nov. 9, 2021

Years of basic neuroscience on the modulation small circuits found in crustacean stomatogastric ganglion have led us to study effects temperature motor patterns produced by ganglion. While impetus for this work was individual variability parameters determining intrinsic and synaptic conductances, we are confronting substantial fluctuations stability networks extreme temperature; these may correlate with changes ocean temperature. Interestingly, when studied under control conditions, wild-caught animals appear be unchanged, but it is only challenged temperatures that reveal consequences warming oceans.

Language: Английский

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The geometry of robustness in spiking neural networks

eLife, Journal Year: 2022, Volume and Issue: 11

Published: May 30, 2022

Neural systems are remarkably robust against various perturbations, a phenomenon that still requires clear explanation. Here, we graphically illustrate how neural networks can become robust. We study spiking generate low-dimensional representations, and show the neurons’ subthreshold voltages confined to convex region in lower-dimensional voltage subspace, which call 'bounding box'. Any changes network parameters (such as number of neurons, dimensionality inputs, firing thresholds, synaptic weights, or transmission delays) all be understood deformations this bounding box. Using these insights, functionality is preserved long perturbations do not destroy integrity suggest principles underlying robustness — heterogeneity tuning, precise negative feedback may key understanding at circuit level.

Language: Английский

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Two central pattern generators from the crab, Cancer borealis, respond robustly and differentially to extreme extracellular pH

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Dec. 28, 2018

The activity of neuronal circuits depends on the properties constituent neurons and their underlying synaptic intrinsic currents. We describe effects extreme changes in extracellular pH – from 5.5 to 10.4 two central pattern generating networks, stomatogastric cardiac ganglia crab, Cancer borealis. Given that physiological ion channels are known be sensitive within range tested, it is surprising these rhythms generally remained robust 6.1 8.8. sensitivity was highly variable between animals and, unexpectedly, ganglia. Animal-to-animal variability likely a consequence similar network performance arising sets conductances. Together, results illustrate potential difficulty generalizing environmental perturbation across circuits, even same animal.

Language: Английский

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In vivo effects of temperature on the heart and pyloric rhythms in the crab, Cancer borealis

Journal of Experimental Biology, Journal Year: 2019, Volume and Issue: unknown

Published: Jan. 1, 2019

The heart and pyloric rhythms of crustaceans have been studied separately extensively over many years. Local hormonal neuromodulation sensory inputs onto these central pattern generator circuits play a significant role in an animals’ response to perturbations, but are usually lost or removed during vitro studies. To examine simultaneously the vivo motor output crustacean rhythms, we used photoplethysmography (PPG). In population measured (n=49), rhythm frequencies ranged from 0.3-2.3 Hz. varied 0.2-1.6 We observed weak correlation between rhythms. During multiple hour-long recordings, animals held at controlled temperature showed strong inhibitory bouts which decreased frequency become quiescent frequency. simultaneous responses ramps by heating cooling saline bath while recording both muscle movements. Q10s, critical temperatures (temperatures function is compromised), changes were calculated for each tested. was more robust high than rhythm.

Language: Английский

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Neuronal oscillator robustness to multiple global perturbations

Biophysical Journal, Journal Year: 2021, Volume and Issue: 120(8), P. 1454 - 1468

Published: Feb. 18, 2021

Neuronal activity depends on ion channels and biophysical processes that are strongly differentially sensitive to physical variables such as temperature pH. Nonetheless, neuronal oscillators can be surprisingly resilient perturbations in these variables. We study a three-neuron pacemaker ensemble drives the pyloric rhythm of crab, Cancer borealis. These crabs routinely experience number global perturbations, including changes Although oscillations robust changes, for sufficiently large deviations reversibly breaks down. As increases beyond tipping point, transition silence. Acidic pH also show points, with reliable first tonic spiking, then Surprisingly, robustness only moderately affects robustness. Consistent high animal-to-animal variability circuit parameters, points vary across animals. However, ordering discrete classes transitions at critical conserved. This implies qualitative oscillator dynamics preserved animals despite quantitative parameter variability. A universal model bursting predicts existence types order which they occur.

Language: Английский

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Reciprocally inhibitory circuits operating with distinct mechanisms are differently robust to perturbation and modulation

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Feb. 1, 2022

Reciprocal inhibition is a building block in many sensory and motor circuits. We studied the features that underly robustness reciprocally inhibitory two neuron used dynamic clamp to create circuits from pharmacologically isolated neurons of crab stomatogastric ganglion by injecting artificial graded synaptic (I Syn ) hyperpolarization-activated inward H currents. There continuum mechanisms generate antiphase oscillations, with ‘release’ ‘escape’ at extremes, mixed mode oscillations between these extremes. In release, active primarily controls off/on transitions. escape, inhibited characterized escape release alterations circuit parameters, temperature, neuromodulation. found rely on tight correlations conductances bursting but are resilient temperature increase. Release robust variations fragile The modulatory current MI restores has little effect Perturbations can alter balance oscillations. conclude same perturbation have dramatically different effects depending circuits’ mechanism operation may not be observable basal activity.

Language: Английский

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Energy-efficient network activity from disparate circuit parameters

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(44)

Published: Oct. 24, 2022

Neural circuits can produce similar activity patterns from vastly different combinations of channel and synaptic conductances. These conductances are tuned for specific but might also reflect additional constraints, such as metabolic cost or robustness to perturbations. How do constraints influence the range permissible conductances? Here we investigate how affects parameters neural with in a model pyloric network crab Cancer borealis . We present machine learning method that identify models generate matching experimental data find consume largely amounts energy despite circuit activity. Furthermore, reduced still significant gives rise energy-efficient circuits. then examine space potential tuning strategies low cost. Finally, interaction between temperature robustness. show vary across temperatures changes does not necessarily incur an increased Our analyses efficiency constraining parameters, systems functional, efficient, robust widely disparate sets

Language: Английский

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Mapping circuit dynamics during function and dysfunction

eLife, Journal Year: 2022, Volume and Issue: 11

Published: March 18, 2022

Neural circuits can generate many spike patterns, but only some are functional. The study of how and maintain functional dynamics is hindered by a poverty description circuit across dysfunctional states. For example, although the regular oscillation central pattern generator well characterized its frequency phase relationships between neurons, these metrics ineffective descriptors irregular aperiodic that under perturbation or in disease By recording well-studied pyloric Cancer borealis, we used statistical features times from neurons to visualize patterns generated this variety conditions. This approach captures both variability rhythms diversity atypical single map. Clusters map identify qualitatively different hinting at dynamic states circuit. State probability statistics transitions varied with environmental perturbations, removal descending neuromodulatory inputs, addition exogenous neuromodulators. analysis reveals strong mechanistically interpretable links complex changes collective behavior neural specific experimental manipulations, constrain hypotheses despite architecture perturbations.

Language: Английский

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Switching neuron contributions to second network activity

Journal of Neurophysiology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 10, 2024

Network flexibility is important for adaptable behaviors. This includes neuronal switching, where neurons alter their network participation, including changing from single- to dual-network activity. Understanding the implications of switching requires determining how a neuron interacts with each its networks. Here, we tested 1) whether "home" and second networks, operating via divergent rhythm generation mechanisms, regulate neuron, 2) if recruited modulation intrinsic properties, contributes or pattern in new network. Small, well-characterized feeding-related networks (pyloric, ~1 Hz; gastric mill, ~0.1 Hz) identified modulatory inputs make isolated crab ( Cancer borealis) stomatogastric nervous system (STNS) useful model study switching. In particular, neuropeptide Gly 1 -SIFamide switches lateral posterior (LPG) (2 copies) pyloric-only dual-frequency pyloric/gastric mill (fast/slow) activity LPG properties. Using current injections manipulate activity, found that but not pyloric, regulated intrinsically generated slow bursting. Conversely, selective elimination both using photoinactivation revealed firing frequencies was necessary coordination. However, alone sufficient produce distinct Thus, modulated properties underlying participation may constrain which can Further, recruitment occur states it actively contribute output.

Language: Английский

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