Miniscope-LFOV: A large field of view, single cell resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals DOI Creative Commons
Changliang Guo, Garrett J. Blair, Megha Sehgal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Nov. 22, 2021

Abstract Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present large field view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 × 2.7 mm), single cell resolution neural imaging rats. It has an electrically adjustable working distance up to 3.5 mm±100 μm, incorporates absolute head-orientation sensor, and weighs only 13.9 grams. The MiniLFOV is capable both deep brain cortical been validated rats by simultaneously >1000 GCaMP7s expressing neurons the hippocampal CA1 layer head-fixed ~2000 dorsal cortex through cranial window. also supports optional wire-free operation using novel, data acquisition expansion board. expect this new implementation UCLA Miniscope platform will enable researchers address novel hypotheses concerning function animals.

Language: Английский

Nucleus Accumbens Corticotropin-Releasing Hormone Neurons Projecting to the Bed Nucleus of the Stria Terminalis Promote Wakefulness and Positive Affective State DOI Creative Commons
Gaojie Pan, Bing Zhao, Mutian Zhang

et al.

Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: 40(11), P. 1602 - 1620

Published: July 9, 2024

Abstract The nucleus accumbens (NAc) plays an important role in various emotional and motivational behaviors that rely on heightened wakefulness. However, the neural mechanisms underlying relationship between arousal emotion regulation NAc remain unclear. Here, we investigated roles of a specific subset inhibitory corticotropin-releasing hormone neurons (NAc CRH ) regulating mice. We found increased activity during wakefulness rewarding stimulation. Activation converts NREM or REM sleep to wakefulness, while inhibition these attenuates Remarkably, activation induces place preference response (PPR) decreased basal anxiety level, whereas their inactivation aversion anxious state. are identified as major projection bed stria terminalis (BNST). Furthermore, -BNST pathway similarly induced positive behaviors. Taken together, forebrain promotes associated with affective states.

Language: Английский

Citations

5
The evolution and diversification of sleep DOI
Niels C. Rattenborg, Gianina Ungurean

Trends in Ecology & Evolution, Journal Year: 2022, Volume and Issue: 38(2), P. 156 - 170

Published: Nov. 18, 2022

Language: Английский

Citations

20
Whole‐brain monosynaptic inputs to lateral periaqueductal gray glutamatergic neurons in mice DOI Creative Commons
Wei‐Xiang Ma, Lei Li, Lingxi Kong

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2023, Volume and Issue: 29(12), P. 4147 - 4159

Published: July 9, 2023

Abstract Objective The lateral periaqueductal gray (LPAG), which mainly contains glutamatergic neurons, plays an important role in social responses, pain, and offensive defensive behaviors. Currently, the whole‐brain monosynaptic inputs to LPAG neurons are unknown. This study aims explore structural framework of underlying neural mechanisms neurons. Methods used retrograde tracing systems based on rabies virus, Cre‐LoxP technology, immunofluorescence analysis. Results We found that 59 nuclei projected In addition, seven hypothalamic nuclei, namely area (LH), preoptic (LPO), substantia innominata (SI), medial area, ventral pallidum, posterior globus pallidus, most densely Notably, we discovered through further analysis were colocalized with several markers related neurological functions associated physiological Conclusion received dense projections from hypothalamus, especially such as LH, LPO, SI. input behaviors, show pivotal behaviors regulation by LPAG.

Language: Английский

Citations

11
Gut microbiota: Candidates for a novel strategy for ameliorating sleep disorders DOI
Longyan Li, Tingting Liang, Tong Jiang

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(29), P. 10772 - 10788

Published: July 21, 2023

The aim of this review was to evaluate the feasibility treating sleep disorders using novel gut microbiota intervention strategies. Multiple factors can cause disorders, including an imbalance in microbiota. Studies microbiome-gut-brain axis have revealed bidirectional communication between central nervous system and microbes, providing a more comprehensive understanding mood behavioral regulatory patterns. Changes its metabolites stimulate endocrine, nervous, immune systems, which regulate release neurotransmitters alter activity system, ultimately leading disorders. Here, we main affecting sleep, discuss possible pathways molecular mechanisms interaction microbiota, compare common strategies aimed at improving physiology.

Language: Английский

Citations

11
The parasubthalamic nucleus: A novel eating center in the brain DOI
Mingxuan Lu, Jiayao Zhang, Qi Zhang

et al.

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Journal Year: 2025, Volume and Issue: 136, P. 111250 - 111250

Published: Jan. 1, 2025

Language: Английский

Citations

0
Chemogenetic inhibition of corticotropin releasing hormone neurons in the paraventricular nucleus attenuates traumatic stress-induced deficit of NREM sleep, but not REM sleep in mice DOI Creative Commons

A. Kostin,

Natalia Suntsova,

Sunil Kumar

et al.

Stress, Journal Year: 2025, Volume and Issue: 28(1)

Published: Feb. 16, 2025

Present study was aimed to elucidate the role of corticotropin releasing hormone (CRH) neurons located in paraventricular nucleus hypothalamus (PVN) mechanisms stress-induced insomnia. Experiments were done rodent model traumatic stress, mice exposure predator (rat) odor. Sleep changes associated with this stress first assessed adult male C57BL/6J wild-type (n = 12). The effect chemogenetic silencing CRH within PVN on insomnia examined CRH-ires-Cre using designer receptors exclusively activated by drugs (DREADD) technology. Animals received bilateral injections inhibitory DREADD vector AAV-hSyn-DIO-hM4Di-mCherry 10) or control AAV-hSyn-DIO-mCherry virus into during surgery. intraperitoneal injection clozapine-N-oxide (CNO) prior induction stress. rat odor induced strong long-lasting suppression both non-rapid eye movement (NREM) and rapid (REM) sleep stages experiments. Selective alleviated acute significantly increasing time spent NREM but it did not counteract deficit REM sleep. These findings suggest a specific for CRH-secreting caused whereas is controlled different mechanism.

Language: Английский

Citations

0
Slumber under pressure: REM sleep and stress response DOI
Bernhard Schaefke,

Jingfei Li,

Binghao Zhao

et al.

Progress in Neurobiology, Journal Year: 2025, Volume and Issue: unknown, P. 102771 - 102771

Published: April 1, 2025

Language: Английский

Citations

0
Functional roles of REM sleep DOI Creative Commons
Yasutaka Mukai, Akihiro Yamanaka

Neuroscience Research, Journal Year: 2022, Volume and Issue: 189, P. 44 - 53

Published: Dec. 23, 2022

Language: Английский

Citations

17
Circuit mechanism for suppression of frontal cortical ignition during NREM sleep DOI Creative Commons
Bing Li, Chenyan Ma, Yun‐An Huang

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(26), P. 5739 - 5750.e17

Published: Dec. 1, 2023

Conscious perception is greatly diminished during sleep, but the underlying circuit mechanism poorly understood. We show that cortical ignition—a brain process shown to be associated with conscious awareness in humans and non-human primates—is strongly suppressed non-rapid-eye-movement (NREM) sleep mice due reduced cholinergic modulation rapid inhibition of responses. Brain-wide functional ultrasound imaging cell-type-specific calcium combined optogenetics showed activity propagation from visual frontal cortex markedly NREM strong pyramidal neurons. Chemogenetic activation inactivation basal forebrain neurons powerfully increased decreased visual-to-frontal propagation, respectively. Furthermore, although multiple subtypes dendrite-targeting GABAergic interneurons are more active wakefulness, soma-targeting parvalbumin-expressing sleep. manipulation parvalbumin sleep/wake-dependent ignition modulated by perisomatic

Language: Английский

Citations

10
The Many Unknowns of Partial Sensory Disconnection during Sleep: A Review of the Literature DOI Creative Commons
Chiara Cirelli, Giulio Tononi

Clinical and Translational Neuroscience, Journal Year: 2024, Volume and Issue: 8(1), P. 9 - 9

Published: Jan. 24, 2024

When we are asleep, lose the ability to promptly respond external stimuli, and yet spend many hours every day in this inherently risky behavioral state. This simple fact strongly suggests that sleep must serve essential functions rely on brain going offline, a daily basis, for long periods of time. If these did not require partial sensory disconnection, it would be difficult explain why they performed during waking. Paradoxically, despite its central role defining what does, disconnection remains mystery. We have limited understanding how is implemented along pathways; do know whether same mechanisms apply all modalities, nor extent shared between non-rapid eye movement (NREM) REM sleep. The main goal contribution review some knowns unknowns about as first step fill gap.

Language: Английский

Citations

3