bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: Nov. 22, 2021
Abstract
Imaging
large-population,
single-cell
fluorescent
dynamics
in
freely
behaving
animals
larger
than
mice
remains
a
key
endeavor
of
neuroscience.
We
present
large
field
view
open-source
miniature
microscope
(MiniLFOV)
designed
for
large-scale
(3.6
×
2.7
mm),
single
cell
resolution
neural
imaging
rats.
It
has
an
electrically
adjustable
working
distance
up
to
3.5
mm±100
μm,
incorporates
absolute
head-orientation
sensor,
and
weighs
only
13.9
grams.
The
MiniLFOV
is
capable
both
deep
brain
cortical
been
validated
rats
by
simultaneously
>1000
GCaMP7s
expressing
neurons
the
hippocampal
CA1
layer
head-fixed
~2000
dorsal
cortex
through
cranial
window.
also
supports
optional
wire-free
operation
using
novel,
data
acquisition
expansion
board.
expect
this
new
implementation
UCLA
Miniscope
platform
will
enable
researchers
address
novel
hypotheses
concerning
function
animals.
Neuropharmacology,
Journal Year:
2024,
Volume and Issue:
256, P. 110003 - 110003
Published: May 22, 2024
Neuromodulation
such
as
deep
brain
stimulation
(DBS)
is
advancing
a
clinical
intervention
in
several
neurological
and
neuropsychiatric
disorders,
including
Parkinson's
disease,
dystonia,
tremor,
obsessive-compulsive
disorder
(OCD)
for
which
DBS
already
applied
to
alleviate
severely
afflicted
individuals
of
symptoms.
Tourette
syndrome
drug
addiction
are
two
additional
disorders
trial
or
proposed
treatment.
However,
some
major
remaining
obstacles
prevent
this
from
reaching
its
full
therapeutic
potential.
Side-effects
have
been
reported,
not
all
DBS-treated
relieved
their
One
target
area
electrodes
the
subthalamic
nucleus
(STN)
plays
important
roles
motor,
affective
associative
functions,
with
impact
on
example
movement,
motivation,
impulsivity,
compulsivity,
well
both
reward
aversion.
The
multifunctionality
STN
complex.
Decoding
anatomical-functional
organization
could
enhance
strategic
targeting
human
patients.
located
close
proximity
zona
incerta
(ZI)
para-subthalamic
(pSTN).
Together,
STN,
pSTN
ZI
form
highly
heterogeneous
clinically
area.
Rodent-based
experimental
studies,
opto-
chemogenetics
viral-genetic
tract
tracings,
provide
unique
insight
into
complex
neuronal
circuitries
behavior
high
spatial
temporal
precision.
This
research
field
has
advanced
tremendously
over
past
few
years.
Here,
we
an
inclusive
review
current
literature
pre-clinical
fields
centered
around
laboratory
mice
rats;
three
enigmatic
structures
brought
together
context
relevance
treatment
strategies.
Specific
emphasis
placed
methods
manipulation
behavioral
impact.
Molecular Psychiatry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 4, 2024
Abstract
The
parasubthalamic
nucleus
(PSTN)
is
activated
by
refeeding
after
food
deprivation
and
several
PSTN
subpopulations
have
been
shown
to
suppress
feeding.
However,
no
study
date
directly
addressed
the
role
of
neurons
upon
access
in
control
ensuing
consumption.
Here
we
identify
consumption
latency
as
a
sensitive
behavioral
indicator
activity,
show
that,
hungry
mice,
ensemble
refeeding-activated
drastically
increases
initiate
with
both
familiar
novel,
food,
but
does
not
amount
consumed.
In
thirsty
this
also
delays
sucrose
accelerates
water
consumption,
possibly
reflecting
anticipatory
prandial
thirst,
again
influence
on
fluid
We
next
sought
which
might
be
driving
these
effects,
using
cell-type
pathway-specific
chemogenetic
manipulations.
Our
results
suggest
prominent
Tac1
projecting
central
amygdala
hindrance
feeding
initiation.
While
Crh
delay
mice
ingest
foods,
they
surprisingly
promote
palatable
substances.
Furthermore,
bed
stria
terminalis
accelerate
rehydration
mice.
demonstrate
key
endogenous
activity
drinking
initiation
delineate
specific
circuits
mediating
may
relevance
for
eating
disorders.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: Nov. 22, 2021
Abstract
Imaging
large-population,
single-cell
fluorescent
dynamics
in
freely
behaving
animals
larger
than
mice
remains
a
key
endeavor
of
neuroscience.
We
present
large
field
view
open-source
miniature
microscope
(MiniLFOV)
designed
for
large-scale
(3.6
×
2.7
mm),
single
cell
resolution
neural
imaging
rats.
It
has
an
electrically
adjustable
working
distance
up
to
3.5
mm±100
μm,
incorporates
absolute
head-orientation
sensor,
and
weighs
only
13.9
grams.
The
MiniLFOV
is
capable
both
deep
brain
cortical
been
validated
rats
by
simultaneously
>1000
GCaMP7s
expressing
neurons
the
hippocampal
CA1
layer
head-fixed
~2000
dorsal
cortex
through
cranial
window.
also
supports
optional
wire-free
operation
using
novel,
data
acquisition
expansion
board.
expect
this
new
implementation
UCLA
Miniscope
platform
will
enable
researchers
address
novel
hypotheses
concerning
function
animals.
