Recruitment of pial collaterals and carotid occlusive disease in large-vessel occlusion ischemic stroke DOI Creative Commons
Niklas Helwig, Marlies Wagner, Alexander Seiler

et al.

Frontiers in Neurology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 28, 2024

Background and purpose Despite the fundamental role of pial collateral vessels in limiting progression ischemic tissue injury acute stroke with large vessel occlusion (LVO), addition to fact that abundance varies naturally from person for genetic reasons, there is limited knowledge regarding potential factors contributing inherent interindividual variation supply. As it has been repeatedly hypothesized chronic carotid occlusive disease may favor collateralization, we aimed investigate association between quantitatively assessed leptomeningeal supply pre-existing stenosis patients due LVO. Materials methods Patients proximal middle cerebral artery (MCA) or without additional internal (ICA) were included. The degree was based on signal variance T2*-weighted time series perfusion-weighted magnetic resonance imaging (PWI). stratified into two groups according quantitative status (poor fair good supply). prevalence high-grade ICA (≥70%) evaluated both groups. Results A total 98 (mean age 68.8 ± 16.1 years, n = 52 (53.1%) whom female individuals) MCA and/or included final analysis. Out these patients, 42 had poor supply, while 56 exhibited Additionally, 18 showed ipsilateral stenosis. After classifying entire cohort their vs. supply), no significant difference proportion Specifically, 6 (14.3%) 12 (21.1%) odds ratio (OR) 1.58, a 95% confidence interval (CI) 0.490–5.685 p- value 0.440. In patient cohort, variance-based significantly correlated initial severity ( r −0.360, p < 0.001), baseline core volume −0.362, functional outcomes (score modified Rankin Scale) at discharge −0.367, 0.01). Conclusion this study, performed observer-independent MRI-based assessment We found those influence demographic clinical variables warrants further exploration future studies. related severity, volume, early outcomes.

Language: Английский

Evolucollateral dynamics in stroke: Evolutionary pathophysiology, remodelling and emerging therapeutic strategies DOI

Akansha Sinha,

Mihir Gupta, Sonu Bhaskar

et al.

European Journal of Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Leptomeningeal collaterals (LMCs) are crucial in mitigating the impact of acute ischemic stroke (AIS) by providing alternate blood flow routes when primary arteries obstructed. This article explores evolutionary pathophysiology LMCs, highlighting their critical function and genetic molecular mechanisms governing development remodelling. We address translational challenges applying animal model findings to human clinical scenarios, emphasizing need for further research validate emerging therapies—such as pharmacological agents, gene therapy mechanical interventions—in settings, aimed at enhancing collateral perfusion. Computational modelling emerges a promising method integrating experimental data, which requires precise parameterization empirical validation. introduce ‘Evolucollateral Dynamics’ hypothesis, proposing novel framework that incorporates biology principles into therapeutic strategies, offering new perspectives on circulation. hypothesis emphasizes role predispositions environmental influences circulation, may strategies optimize treatment outcomes. Future must incorporate data create robust protocols, thereby maximizing potential LMCs improving outcomes patients.

Language: Английский

Citations

3
Simultaneous head-mounted imaging of neural and hemodynamic activities at high spatiotemporal resolution in freely behaving mice DOI Creative Commons
Ningbo Chen, Zhiqiang Xu,

Zheng Song

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(12)

Published: March 21, 2025

Exploring the relationship between neural activity and cerebral hemoglobin-oxygenation responses in freely behaving mice can advance our understanding of neurovascular coupling. Head-mounted microscopes enable neuroimaging mice; however, investigating dynamics remains challenging because a lack hemodynamic information, especially blood oxygenation, or insufficient resolution. Here, we report head-mounted microscope for imaging that enables simultaneous recording neuronal burst firing multiparametric hemodynamics such as vascular oxygen saturation at high spatiotemporal The 1.7-gram lightweight integrates confocal fluorescence photoacoustic microscopy, allowing recordings 0.78 hertz with 1.5-micrometer lateral resolution across field view 400 micrometers by micrometers. We identified cell type–specific to hypoxic challenges, observed active regulation arterioles during sensory stimuli, detected abnormal depletion vasodilation preceding discharges epileptic disorders. This technique provides valuable insights into coupling holds potential studying pathology neurological brain diseases.

Language: Английский

Citations

0
Interaction of brain imaging features and effects of intensive blood pressure lowering after endovascular treatment for acute ischaemic stroke: the pre-specified secondary analyses of ENCHANTED2/MT trial DOI
Xiaoxi Zhang, Xinwen Ren, Yongxin Zhang

et al.

EClinicalMedicine, Journal Year: 2025, Volume and Issue: 83, P. 103197 - 103197

Published: April 21, 2025

Language: Английский

Citations

0
Artery regeneration: Molecules, mechanisms and impact on organ function DOI
Swarnadip Ghosh,

Bhavnesh Bishnoi,

Soumyashree Das

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103611 - 103611

Published: May 2, 2025

Language: Английский

Citations

0
The impact of cerebral vessels morphological alteration and white matter hyperintensities burden on the one-year risk of ischemic stroke recurrence DOI Creative Commons
Hao Wang,

Guoqing Wu,

Bei Wang

et al.

BMC Medical Imaging, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 5, 2025

Language: Английский

Citations

0
Acute Ischemic Stroke in the Clinic and the Laboratory: Targets for Translational Research DOI Creative Commons
Bart A. A. Franx, Rick M. Dijkhuizen,

Diederik W.J. Dippel

et al.

Neuroscience, Journal Year: 2024, Volume and Issue: 550, P. 114 - 124

Published: April 24, 2024

Highlights•Advancements in ischemic stroke research introduce new treatment challenges.•This narrative review selectively highlights advances with clinical impact using a fictional case.•Outstanding questions are addressed that can benefit from experimental-clinical collaborations.AbstractIschemic has enabled significant advancements diagnosis, treatment, and management of this debilitating disease, yet challenges remain standing the way better patient prognoses. In review, case illustrates uncertainties medical professionals still face – penumbra identification, lack neuroprotective agents, side-effects tissue plasminogen activator, dearth molecular biomarkers, incomplete microvascular reperfusion or no-reflow, post-recanalization hyperperfusion, blood pressure procedural anesthetic effects. The current state field is broadly reviewed per topic, aim to broad audience (scientist clinician alike) recent successes translational pending scientific queries tractable for preclinical assessment. Opportunities co-operation between experimental experts highlighted increase size frequency strides makes improve our understanding disease ways treating it.

Language: Английский

Citations

2
Collateral is brain: Low perfusion triggers spreading depolarization and futile reperfusion after acute ischemic stroke DOI
Anna Törteli, Réka Tóth, Ferenc Bari

et al.

Journal of Cerebral Blood Flow & Metabolism, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Futile reperfusion is a phenomenon of inadequate perfusion despite successful recanalization after acute ischemic stroke (AIS). It associated with poor patient outcomes and has received increasing interest due to its clinical diagnosis becoming more common. However, the underlying mechanisms remain elusive, experimental studies are focused on pathological background futile reperfusion. Our recent study confirmed that primary collateralization plays crucial role in insufficiency AIS mice. Specifically, absence collaterals circle Willis (CoW) promoted development spreading depolarizations (SDs) during AIS. In our model, occurrence SDs ischemia always predicted Conversely, mice complete CoW, no were observed, was complete. Importantly, human CoW displays variation approximately 50% population. Therefore, may result from SD evolution patients. purpose here emphasize We propose adequate collateral recruitment can prevent occurrence, leading improved outcomes.

Language: Английский

Citations

2
Transcranial Cortex-Wide Imaging of Murine Ischemic Perfusion With Large-Field Multifocal Illumination Microscopy DOI
Zhenyue Chen, Quanyu Zhou,

Jeanne Droux

et al.

Stroke, Journal Year: 2024, Volume and Issue: 56(1), P. 170 - 182

Published: Dec. 20, 2024

BACKGROUND: Ischemic stroke is a common cause of death worldwide and main morbidity. Presently, laser speckle contrast imaging, x-ray computed tomography, magnetic resonance imaging are the mainstay for diagnosis therapeutic monitoring in preclinical studies. These modalities often limited terms their ability to map brain perfusion with sufficient spatial temporal resolution, thus calling development new techniques featuring rapid speed, cost-effectiveness, ease use. METHODS: We report on high-resolution angiography technique murine ischemic based large-field high-speed multifocal illumination fluorescence microscopy. subsequently showcase proposed method by effects thrombolysis (n=6), further performing cross-strain comparison dynamics (n=6) sensory stimulation–based treatment (n=11). RESULTS: Quantitative readings hemodynamic structural changes cerebral vascular network pial vessels were attained 14.4-µm resolution at 80-Hz frame rate fully transcranially. The vivo maps accurately delineated core penumbra, exhibiting strong correlation (86.1±4.5%) ex triphenyl tetrazolium chloride staining, significantly higher than conventional method. Monitoring confirmed that early recanalization could effectively save penumbra while reducing infarct area. Cross-strain affirmed C57BL/6 mice feature larger smaller as compared BALB/c mice, which have few or no collaterals. Sensory enhance blood flow abolish deficits regions. CONCLUSIONS: A fluorescence-based transcranial introduced, capable localizing assessing penumbra. Compared whole-brain tomography methods, conventionally used monitoring, approach simple cost-effective, offering high speed It opens venues research under various disease conditions such stroke, neurodegeneration, epileptic seizures.

Language: Английский

Citations

2
The role of leptomeningeal collaterals in redistributing blood flow during stroke DOI Creative Commons
Robert Epp, Chaim Glück, Nadine Felizitas Binder

et al.

PLoS Computational Biology, Journal Year: 2023, Volume and Issue: 19(10), P. e1011496 - e1011496

Published: Oct. 23, 2023

Leptomeningeal collaterals (LMCs) connect the main cerebral arteries and provide alternative pathways for blood flow during ischaemic stroke. This is beneficial reducing infarct size reperfusion success after treatment. However, a better understanding of how LMCs affect distribution indispensable to improve therapeutic strategies. Here, we present novel in silico approach that incorporates case-specific vivo data into computational model simulate large semi-realistic microvascular networks from two different mouse strains, characterised by having many almost no between middle anterior artery (MCA, ACA) territories. framework unique because our simulations are directly aligned with data. Moreover, it allows us analyse perfusion characteristics quantitatively across all vessel types no, few LMCs. We show occlusion MCA caused redistribution was increased Interestingly, improved MCA-sided microvessels dilating came at cost reduced supply other brain areas. effect enhanced regions close watershed line when number increased. Additional dilations surface penetrating stroke entire vasculature partially recovered obstructed region, especially LMCs, which further underlines role

Language: Английский

Citations

6
Pia-FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its response to arterial occlusion DOI Creative Commons
Chaim Glück, Quanyu Zhou,

Jeanne Droux

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(28)

Published: July 2, 2024

The pial vasculature is the sole source of blood supply to neocortex. brain contained within skull, a vascularized bone marrow with unique anatomical connection meninges. Recent developments in tissue clearing have enabled detailed mapping entire and calvarial vasculature. However, what are absolute flow rate values those vascular networks? This information cannot accurately be retrieved commonly used bioimaging methods. Here, we introduce Pia-FLOW, approach based on large-scale transcranial fluorescence localization microscopy, attain hemodynamic imaging whole murine at frame rates up 1,000 Hz spatial resolution reaching 5.4 µm. Using provide maps velocity, direction, diameters which can serve as ground-truth data for further studies, advancing our understanding fluid dynamics. Furthermore, Pia-FLOW revealed that network functions one unit robust allocation after stroke.

Language: Английский

Citations

1