START method assembles brain’s wiring diagram by cell type DOI
H.A. Barker

The Transmitter, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Neocortical somatostatin neuron diversity in cognition and learning DOI Creative Commons
Eunsol Park, Matthew B. Mosso, Alison L. Barth

et al.

Trends in Neurosciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Somatostatin neurons detect stimulus-reward contingencies to reduce neocortical inhibition during learning DOI
Eunsol Park, Dika Kuljis,

Rachel A Swindell

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(5), P. 115606 - 115606

Published: April 20, 2025

Language: Английский

Citations

0
High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping Using Single-Cell and Single-Nucleus Sequencing DOI Creative Commons
David Shin, M Urbanek,

H. Hanh Larson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

SUMMARY Single cell genomics has revolutionized our understanding of neuronal types. However, scalable technologies for probing single-cell connectivity are lacking, and we just beginning to understand how molecularly defined types organized into functional circuits. Here, describe a protocol generate high-complexity barcoded rabies virus (RV) circuit mapping from tens thousands individual starter cells in parallel. In addition, introduce strategy targeting RV-encoded barcode transcripts the nucleus so that they can be read out using single-nucleus RNA sequencing (snRNA-seq). We apply this tool organotypic slice cultures developing human cerebral cortex, which reveals emergence type– specific motifs midgestation. By leveraging power throughput single synaptic connectivity, chart path forward molecularly-defined healthy disease states.

Language: Английский

Citations

1
Transcriptomic diversity of amygdalar subdivisions across humans and nonhuman primates DOI Creative Commons
Michael S. Totty, Rita Cervera‐Juanes, Svitlana V. Bach

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 19, 2024

The amygdaloid complex mediates learning, memory, and emotions. Understanding the cellular anatomical features that are specialized in amygdala of primates versus other vertebrates requires a systematic, anatomically-resolved molecular analysis constituent cell populations. We analyzed five nuclear subdivisions primate with single-nucleus RNA sequencing macaques, baboons, humans to examine gene expression profiles for excitatory inhibitory neurons confirmed our results single-molecule FISH analysis. identified distinct subtypes

Language: Английский

Citations

1
START method assembles brain’s wiring diagram by cell type DOI
H.A. Barker

The Transmitter, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0