Solid-phase microextraction with recessed matrix compatible coating for in situ sampling of per- and polyfluoroalkyl substances in meat DOI Creative Commons
Yaping Li, Wei Zhou, Runshan Will Jiang

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 480, P. 143891 - 143891

Published: March 15, 2025

This study presents a novel method for in situ extraction of per- and polyfluoroalkyl substances (PFAS) from intact meat samples using recessed solid phase microextraction (SPME) device coupled with LC-MS/MS. The SPME matrix-compatible coating (HLB-WAX/PAN) the section, exhibited mechanically robust low matrix effects (-13.7-11.1 %). Key parameters influencing efficiency, including time, adsorbent amount, temperature, desorption time were comprehensively optimized. stability PFAS adsorbed onto during storage at different temperatures durations was also assessed. Under optimized conditions, proposed demonstrated applicability across pork, beef, lamb tissues excellent linearity (R2 ≥ 99.32 %), good sensitivity (LOD range 0.01-1.52 ng/g), as well acceptable accuracy reproducibility (intra-day inter-day). Compared conventional methods, SPME-LC-MS/MS shows advantages simple operation, short organic solvent consumption effects. approach offers straightforward reliable solution direct monitoring commercial has potential on-site application.

Language: Английский

Nontarget Analysis of Legacy and Emerging PFAS in a Lithium-Ion Power Battery Recycling Park and Their Possible Toxicity Measured Using High-Throughput Phenotype Screening DOI
Zenghua Qi,

Yutian Cao,

Dan Li

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(32), P. 14530 - 14540

Published: July 17, 2024

Driven by the global popularity of electric vehicles and shortage critical raw materials for batteries, spent lithium-ion power battery (LIPB) recycling industry has exhibited explosive growth in both quantity scale. However, relatively little information is known about environmental risks posed LIPB recycling, particular with regards to perfluoroalkyl polyfluoroalkyl substances (PFAS). In this work, suspect screening nontarget analysis were carried out characterize PFAS soil, dust, water sediment from a area. Twenty-five nine classes identified at confidence level 3 or above, including 13 legacy 12 emerging PFAS, as well two ultrashort-chain PFAS. Based on target 16 least detected each sample, indicating their widespread presence Perfluorodecanoic acid, perfluorooctanesulfonic acid trifluoromethanesulfonamide showed significant differences four phenotypic parameters (growth, movement, survival fecundity)

Language: Английский

Citations

9
Association Between Serum Levels of Perfluoroalkyl and Polyfluoroalkyl Substances and Dental Floss Use: The Double‐Edged Sword of Dental Floss Use—A Cross‐Sectional Study DOI Creative Commons

Yan Jiao,

Fu Zhuo,

Xiaochen Ni

et al.

Journal Of Clinical Periodontology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 11, 2025

ABSTRACT Background Although evidence suggests that dental floss contains perfluoroalkyl and polyfluoroalkyl substances (PFASs), it is still uncertain whether the use of contributes to an increased risk PFAS exposure. Methods We analysed data on serum concentrations usage in a cohort 6750 adults who participated National Health Nutrition Examination Survey (NHANES) from 2009 2020. In our study, we used logistic regression, survey‐weighted linear model, item response theory (IRT) scores, inverse probability weights (IPWs) sensitivity analysis assess potential impact human levels. Results The six PFASs revealed users had higher perfluorooctanoic acid (PFOA) compared with non‐users, while other were lower. Dental recorded lower level overall burden score non‐users. Sensitivity showed statistically significant increase PFOA concentration among users. Conclusion Our findings suggest may be associated differently specific PFASs. Among large representative sample U.S. adults, individuals reporting levels overall, exception PFOA, which was slightly elevated. important oral hygiene tool, further research needed clarify its role

Language: Английский

Citations

1
Broad PFAS binding with fatty acid binding protein 4 is enabled by variable binding modes. DOI Creative Commons
Aaron S. Birchfield, Faik N. Musayev,

Abdul J. Castillo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract Per- and polyfluoroalkyl substances (PFAS) are ubiquitous pollutants that bioaccumulate in wildlife humans, yet the molecular basis of their protein interactions remains poorly understood. Here, we show human adipocyte fatty acid-binding (FABP4) can bind a diverse array PFAS, including next-generation replacements for legacy chemicals longer-chain perfluorocarboxylic acids. Shorter-chain although weaker binders, still displayed measurable affinities—surpassing those nonfluorinated analogs. We determined crystal structures FABP4 bound to perfluorooctanoic acid (PFOA), perfluorodecanoic (PFDA), perfluorohexadecanoic (PFHxDA), revealing three distinct binding modes. Notably, PFOA binds two separate sites, conformations define single-ligand PFDA PFHxDA. These arrangements enhance hydrophobic within cavity likely explain low micromolar dissociation constants observed fluorescence competition assays. Our findings underscore critical roles chain length, headgroup functionality, conformation PFAS–FABP4 interactions. Given emerging implications role endocrine function, even subtle PFAS-induced perturbations could affect metabolic regulation disease risk. Overall, this work highlights value direct structural biochemical insights into paves way future research on PFAS transport toxicological outcomes.

Language: Английский

Citations

1
The sources and bioaccumulation of per- and polyfluoroalkyl substances in animal-derived foods and the potential risk of dietary intake DOI
Yudong Xing, Yan Zhou, Xin Zhang

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 905, P. 167313 - 167313

Published: Sept. 22, 2023

Language: Английский

Citations

20
Harnessing carbon-based adsorbents for poly- and perfluorinated substance removal: A comprehensive review DOI Creative Commons
Antônio Carlos Severo Menezes, Louella Concepta Goveas, Ramesh Vinayagam

et al.

Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 69, P. 106621 - 106621

Published: Nov. 22, 2024

Language: Английский

Citations

7
Impact of perfluorooctanoic acid (PFOA) and perfluorobutanoic acid (PFBA) on oxidative stress and metabolic biomarkers in human neuronal cells (SH-SY5Y) DOI Creative Commons

Precious Obiako,

Solomon Ayisire,

Christie M. Sayes

et al.

Environment International, Journal Year: 2024, Volume and Issue: 190, P. 108864 - 108864

Published: July 2, 2024

Perfluorinated alkyl substances (PFAS) are pervasive environmental contaminants that have attracted considerable attention due to their widespread utilization, resilient characteristics, adverse health implications, and regulatory scrutiny. Despite documented toxicity in living organisms, the precise molecular mechanisms governing induced effects remain unclear. This study aims elucidate of toxic action by collecting empirical data sets along oxidative stress metabolic disruption pathways. We investigated impact long-chain PFAS (perfluorooctanoic acid (PFOA)) its short-chain analog (perfluorobutanoic (PFBA)) on human neuronal cells (SH-SY5Y). The functionalities enzymes associated with (catalase glutathione reductase) cellular metabolism (lactate dehydrogenase pyruvate dehydrogenase) were also characterized. Our results reveal a 24-hour exposure PFOA PFBA generated significant levels reactive oxygen species. Correspondingly, there was notable decline catalase reductase activities, demonstrating more pronounced effect. High concentrations reduced activity. Lactate activity only impacted high concentration PFBA, while decreased increased exposure. findings from this contribute knowledge cell interactions potential underlying PFAS-induced toxicity.

Language: Английский

Citations

5
Effect of physicochemical parameters on the occurrence of per- and polyfluoroalkyl substances (PFAS) in aquatic environment DOI Creative Commons
Chinemerem Ruth Ohoro, James F. Amaku, Jeanet Conradie

et al.

Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 208, P. 117040 - 117040

Published: Oct. 3, 2024

Language: Английский

Citations

5
Suspect and nontarget screening of per- and polyfluoroalkyl substances based on ion mobility mass spectrometry and machine learning techniques DOI

Hongxin Mu,

Zhongchao Yang,

Ling Chen

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 461, P. 132669 - 132669

Published: Sept. 29, 2023

Language: Английский

Citations

11
Countermeasure for interfered monitoring ion of perfluorooctanesulfonic acid (PFOS) from intrinsic food samples based on LC-MS/MS analysis of per- and polyfluoroalkyl substances DOI Creative Commons
Takahiro Takayama,

A. Shingu,

Sumire Kato

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 133, P. 106436 - 106436

Published: June 20, 2024

This study aimed to investigate the risk of false liquid chromatography with tandem mass spectrometry (LC-MS/MS) detection (electrospray ionization-positive mode, m/z 499 > 80) perfluorooctanesulfonic acid (PFOS) caused by interference peak from food matrices for analysis per- and polyfluoroalkyl substances (PFAS). A complete separation between PFOS peaks was achieved using conventional analytical conditions mobile phase based on C18 column 5 mmoL L-1 ammonium acetate containing water/acetonitrile. Moreover, chemical is identified taurochenodeoxycholic egg sample. Then, a variety approaches have been used quantify PFAS C4-C9 perfluoro chain type solid extraction mixed standard (PFAC-MXC). The amounts in certified fish paste were determined within uncertainty level (95% confidence interval). Among four types foods (meat, vegetable, fish, egg), these well recovered (within around 80%–120%) good reproducibility (RSD (%) < 15%). Our approach suitable quantifying chains matrices.

Language: Английский

Citations

4
Revealing Hidden Risks: In Vitro Analysis of PFAS Hazards in Mytilus galloprovincialis Gills and Digestive Gland DOI Creative Commons
Marta Cunha, Alessandro Nardi, Amadeu M.V.M. Soares

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 485, P. 136823 - 136823

Published: Dec. 9, 2024

Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals known for their persistence bioaccumulation, leading to widespread environmental contamination. Despite recognised risks, particularly aquatic wildlife, including marine invertebrates, detailed impact studies limited. PFAS can be categorised according the length of compound chain, with short-chain announced as a safer alternative more commonly used long-chain PFAS. However, recent evidence suggests that also pose significant risks. The present study evaluated adverse effects six compounds-two (PFHxA, 6:2 FTA) four (PFUnDA, PFDoA, PFTriDA, PFTeDA)- on digestive gland gills mussels, Mytilus galloprovincialis, using in vitro assays. results showed organ-specific responses: was sensitive PFHxA, increased catalase activity decreased total antioxidant capacity, cellular damage observed only at higher concentrations PFTriDA. Gills were affected by PFDoA PFTeDA, inhibited enzyme oxidative stress. PFHxA PFTriDA inhibition acetylcholinesterase activity. FTA had lowest both organs, while most harmful. These findings underscore need thorough risk assessments PFAS, considering chain effects.

Language: Английский

Citations

4