Photoacoustics, Journal Year: 2025, Volume and Issue: 42, P. 100703 - 100703
Published: Feb. 20, 2025
Language: Английский
Light Science & Applications, Journal Year: 2022, Volume and Issue: 11(1)
Published: May 12, 2022
A superresolution imaging approach that localizes very small targets, such as red blood cells or droplets of injected photoacoustic dye, has significantly improved spatial resolution in various biological and medical modalities. However, this superior is achieved by sacrificing temporal because many raw image frames, each containing the localization target, must be superimposed to form a sufficiently sampled high-density image. Here, we demonstrate computational strategy based on deep neural networks (DNNs) reconstruct images from far fewer frames. The can applied for both 3D label-free optical-resolution microscopy (OR-PAM) 2D labeled computed tomography (PACT). For former, required number volumetric frames reduced tens than ten. latter, 12 fold. Therefore, our proposed method simultaneously (via DNN) method) resolutions tomography. Deep-learning powered PA potentially provide practical tool preclinical clinical studies requiring fast fine resolutions.
Language: Английский
Citations
84
Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 184, P. 114235 - 114235
Published: March 25, 2022
Language: Английский
Citations
70
Biomedical Engineering Letters, Journal Year: 2021, Volume and Issue: 12(1), P. 19 - 35
Published: Dec. 19, 2021
Language: Английский
Citations
58
Photoacoustics, Journal Year: 2022, Volume and Issue: 27, P. 100374 - 100374
Published: May 20, 2022
Photoacoustic microscopy (PAM) is used to visualize blood vessels and monitor their time-dependent changes. Photoplethysmography (PPG) measures hemodynamic time-series changes such as heart rate. However, PPG's limited visual access the dynamic of has prohibited further understanding hemodynamics. Here, we propose a novel, fully integrated PAM photoplethysmography (PAM-PPG) system understand features in detail. Using PAM-PPG system, simultaneously acquire vascular images (by PAM) volume PPG) from human fingers. Next, determine rate PA signals, which match well with PPG signals. These can be measured if flow not blocked. From results, believe that could useful clinical tool various fields cardiology endocrinology.
Language: Английский
Citations
49
Photoacoustics, Journal Year: 2023, Volume and Issue: 30, P. 100485 - 100485
Published: March 30, 2023
Postprandial hyperglycemia, blood glucose spikes, induces endothelial dysfunction, increasing cardiovascular risks. Endothelial dysfunction leads to vasoconstriction, and observation of this phenomenon is important for understanding acute hyperglycemia. However, high-resolution imaging microvessels during hyperglycemia has not been fully developed. Here, we demonstrate that photoacoustic microscopy can noninvasively monitor morphological changes in vessels live animals' extremities when rises rapidly. As level rose from 100 400 mg/dL following intraperitoneal injection, heart/breath rate, body temperature remained constant, but arterioles constricted by approximately -5.7 ± 1.1% within 20 min, gradually recovered another 40 min. In contrast, venular diameters about 0.6 1.5% arteriolar constriction. Our results experimentally statistically produces transitory vasoconstriction arterioles, with an opposite trend change glucose. These findings could help vascular homeostasis the relationship between diabetes diseases.
Language: Английский
Citations
30
Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)
Published: Sept. 2, 2024
Language: Английский
Citations
15
Chinese Journal of Lasers, Journal Year: 2024, Volume and Issue: 51(1), P. 0107003 - 0107003
Published: Jan. 1, 2024
光学技术在生物医学中扮演着越来越重要的角色,其非电离辐射、高分辨率、高对比度和对生物组织异变高度灵敏等特性使其非常适用于生物组织的研究,包括成像、传感、治疗、刺激以及控制等。然而由于光折射因子在生物组织中的分布是不均匀的,光在生物组织中的传播会受到很强的散射影响,故纯光学技术的穿透深度和空间分辨率是"鱼和熊掌不可兼得";高分辨率光学成像应用仅限于样品浅表层,当成像深度增加时分辨率急剧下降。实现光在深层生物组织里的高分辨率成像或应用是人们期盼已久的目标。近年来,为解决这一问题,研究者提出了不同的方法,例如切换到更长的光波长以减小组织散射系数,在信号检测时将漫射光转换为散射不明显的超声信号,逆转或者预先补偿由光的多次散射所带来的相位畸变,或借助光纤等微创光学通道实现深层生物组织的高分辨率光学成像、刺激等。基于团队在深层生物组织光学相关领域多年的耕耘,从光在生物组织中的传播特性出发,梳理和总结了近年来研究人员在光-声结合和光学波前整形技术等方面展开的诸多探索,以及在生物组织操控、成像、光学计算以及人工智能等领域中的应用尝试。虽然尚有诸多不足,但随着硬件设备的更新和计算技术的发展,在不远的将来有望实现活体深层生物组织光学高分辨率应用。在这一求索过程中,新方法和新能力将不断激发新的应用灵感,为光学尤其是生物医学光子学带来全新的理念和机遇。
Citations
9
Photoacoustics, Journal Year: 2025, Volume and Issue: 42, P. 100687 - 100687
Published: Jan. 9, 2025
Photoacoustic microscopy (PAM) leverages the photoacoustic effect to provide high-resolution structural and functional imaging. However, achieving high-speed imaging with high spatial resolution remains challenging. To address this, undersampling deep learning have emerged as common techniques enhance speed. Yet, existing methods rarely achieve effective recovery of images. In this study, we propose Mask-enhanced U-net (MeU-net) for recovering sparsely sampled PAM The model utilizes dual-channel input, processing data from 532 nm 558 wavelengths. Additionally, introduce an adaptive vascular attention mask module that focuses on information design a vessel-specific loss function restoration accuracy. We simulate mouse brain ear under various levels sparsity (4 ×, 8 12 ×) conduct extensive experiments. results demonstrate MeU-net significantly outperforms traditional interpolation other representative models in oxygen saturation recovery.
Language: Английский
Citations
1
Biomedical Engineering Letters, Journal Year: 2021, Volume and Issue: 12(2), P. 155 - 173
Published: Nov. 23, 2021
Language: Английский
Citations
43
Biomedical Engineering Letters, Journal Year: 2022, Volume and Issue: 12(2), P. 97 - 124
Published: April 10, 2022
Language: Английский
Citations
35