Heliyon,
Journal Year:
2024,
Volume and Issue:
10(9), P. e29856 - e29856
Published: April 22, 2024
The
article
divulges
the
crystal
growth,
synthesis,
and
X-ray
structure
characterization
of
one
centrosymmetric
cadmium
complex,
[Cd{CdL(μ2-1,3-acetate)}2]
using
Salen
ligand
(SL).
complex
is
further
characterized
spectroscopic
analytical
techniques,
including
DRS,
SEM-EDX,
PXRD,
ICP-MS.
crystallographic
study
showed
that
has
a
monoclinic
space
P21/c.
Addison
parameters
(Ʈ)
show
hexagonal
geometry
central
Cd(II)
metal
ion.
Hirshfeld
surface
2-D
fingerprint
confirm
supramolecular
contacts
despite
weak
C−H···O
C−H···π
interactions.
Energy
frameworks,
FMOs,
global
reactivity
parameters,
MEP,
energy
bandgap
explain
outlook.
inter-
intramolecular
bonding
interactions
were
explored
through
natural
bond
orbital
(NBO),
QTAIM,
NCI-RDG,
Electron
Location
Function
(ELF),
Localized
Orbital
Locator
(LOL)
quantization
methods.
In
addition,
its
synthetic
components
in
vitro
antibacterial
efficacy
investigated
Gram-positive
Gram-negative
microbial
strains.
SAR
(structure-activity
relationship)
correlates
with
biological
potency.
Molecular
docking
assessed
antimicrobial
potency
proteins
S.
aureus
(PDB
ID:
1JIJ),
C.
albicans
1M7A),
E.
coli
1T9U),
P.
aeruginosa
2UV0),
A.
Niger
3K4P).
findings
are
backed
by
Protein-Ligand
Interaction
Profiler
(PLIP).
antifungal
cell
viability
test
conducted
photodynamic
therapy
(APDT).
Biofilm,
Journal Year:
2023,
Volume and Issue:
5, P. 100126 - 100126
Published: April 24, 2023
Virtually
all
Candida
species
linked
to
clinical
candidiasis
are
capable
of
forming
highly
resistant
biofilms
on
different
types
surfaces,
which
poses
an
additional
significant
threat
and
further
complicates
therapy
these
infections.
There
is
a
scarcity
antifungal
agents,
their
effectiveness,
particularly
against
biofilms,
limited.
Here
we
provide
historical
perspective
agents
biofilms.
As
reflect
upon
the
past,
consider
present,
look
towards
future
believe
that
there
reasons
remain
optimistic,
major
challenges
biofilm
can
be
conquered
within
reasonable
timeframe.
Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
17(5), P. 548 - 548
Published: April 23, 2025
Background/Objectives:
Parietin
(PTN),
a
blue-light
absorbing
pigment
from
Teloschistes
spp.
lichens,
exhibit
photosensitizing
properties
via
Type
I
(superoxide
anion,
O2•−)
and
II
(singlet
oxygen,
1O2)
mechanisms,
inactivating
bacteria
in
vitro
after
photoexcitation.
We
evaluate
the
antifungal
activity
of
PTN
against
Candida
tropicalis
biofilms
under
actinic
irradiation,
its
role
O2•−
1O2
production,
cellular
stress
response.
Methods:
Minimum
inhibitory
concentration
(MIC)
was
determined
C.
NCPF
3111
dark
light
conditions.
Biofilm
susceptibility
assessed
at
MIC/2,
MIC,
MICx2,
MICx4,
MICx6
same
conditions,
viability
measured
by
colony-forming
units.
Photodynamic
mechanisms
were
examined
using
Tiron
(O2•−
scavenger)
or
sodium
azide
(1O2
quencher).
production
nitro-blue
tetrazolium
(NBT)
reduction
nitric
oxide
(NO)
generation
Griess
assay.
Total
antioxidant
capacity
studied
FRAP
(Ferrous
Reduction
Antioxidant
Potency)
assay
superoxide
dismutase
(SOD)
NBT
Results:
Photoexcitation
reduced
biofilm
four
logs
MICx2.
Sodium
partially
reversed
effect,
whereas
fully
inhibited
it,
indicating
critical
O2•−.
also
increased
NO
levels,
enhancing
SOD
FRAP.
However,
this
response
insufficient
to
prevent
photoinactivation.
Conclusions:
Photoinactivation
is
primarily
mediated
O2•−,
with
minor
contribution
an
imbalance
levels.
These
findings
suggest
promising
photosensitizer
for
photodynamic
therapy.
Pharmaceutics,
Journal Year:
2023,
Volume and Issue:
15(5), P. 1511 - 1511
Published: May 16, 2023
Onychomycosis
is
a
prevalent
nail
fungal
infection,
and
Candida
albicans
one
of
the
most
common
microorganisms
associated
with
it.
One
alternative
therapy
to
conventional
treatment
onychomycosis
antimicrobial
photoinactivation.
This
study
aimed
evaluate
for
first
time
in
vitro
activity
cationic
porphyrins
platinum(II)
complexes
4PtTPyP
3PtTPyP
against
C.
albicans.
The
minimum
inhibitory
concentration
reactive
oxygen
species
was
evaluated
by
broth
microdilution.
yeast
eradication
using
time-kill
assay,
checkerboard
assay
assessed
synergism
combination
commercial
treatments.
In
biofilm
formation
destruction
were
observed
crystal
violet
technique.
morphology
samples
atomic
force
microscopy,
MTT
technique
used
cytotoxicity
studied
keratinocyte
fibroblast
cell
lines.
porphyrin
showed
excellent
antifungal
tested
strains.
After
white-light
irradiation,
eradicated
growth
30
60
min.
possible
mechanism
action
mixed
ROS
generation,
combined
drugs
indifferent.
significantly
reduced
preformed
vitro.
Lastly,
microscopy
cellular
damage
samples,
did
not
show
We
conclude
that
an
photosensitizer
promising
results
Microorganisms,
Journal Year:
2023,
Volume and Issue:
11(6), P. 1455 - 1455
Published: May 31, 2023
Galleria
mellonella
is
a
promising
in
vivo
model
insect
used
for
microbiological,
medical,
and
pharmacological
research.
It
provides
platform
testing
the
biocompatibility
of
various
compounds
kinetics
survival
after
an
infection
followed
by
subsequent
treatment,
evaluation
parameters
during
including
host–pathogen
interaction.
There
are
some
similarities
development
pathologies
with
mammals.
However,
limitation
lack
adaptive
immune
response.
Antimicrobial
photodynamic
therapy
(aPDT)
alternative
approach
combating
microbial
infections,
biofilm-associated
ones.
aPDT
effective
against
Gram-positive
Gram-negative
bacteria,
viruses,
fungi,
parasites,
regardless
whether
they
resistant
to
conventional
treatment.
The
main
idea
this
comprehensive
review
was
collect
information
on
use
G.
aPDT.
collection
references
published
last
10
years
from
area
research,
complemented
practical
experiences
authors
review.
Additionally,
summarizes
brief
model,
its
advantages
methods
processing
material
these
larvae,
as
well
basic
knowledge
principles
