Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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The contribution of the genomes of a termite and a locust to our understanding of insect neuropeptides and neurohormones

Frontiers in Physiology, Journal Year: 2014, Volume and Issue: 5

Published: Nov. 19, 2014

The genomes of the migratory locust Locusta migratoria and termite Zootermopsis nevadensis were mined for presence genes encoding neuropeptides, neurohormones, their G-protein coupled receptors (GPCRs). Both species have retained a larger number neuropeptide GPCRs than better known holometabolous insect species, while other that in appear to single transcript produce two different precursors locust, or both. Thus, recently discovered CNMa gene has transcripts predicted structurally peptides termite, produces myosuppressin same fashion. these also calcitonin gene, which is from calcitonin-like diuretic hormone. This types calcitonins, calcitonins A B. It present Lepidoptera Coleoptera some Diptera, but absent mosquitoes Drosophila. However, only B produced. Their putative identified. In contrast, highly unusual codes salivation stimulatory peptide. neuroparsin vasopressin are particularly interesting. five transcripts, one neurohormone identified corpora cardiaca. four code neuroparsin-like proteins, lack amino acid residues, reason we called neoneuroparsins. neoneuroparsins about 200 times transcripts. first exon promoter genes, there seven copies genome, very well-conserved, remainder not. relevance findings discussed.

Language: Английский

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Similarities between decapod and insect neuropeptidomes

PeerJ, Journal Year: 2016, Volume and Issue: 4, P. e2043 - e2043

Published: May 26, 2016

Background. Neuropeptides are important regulators of physiological processes and behavior. Although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression significant differences between species, raising question whether such real or artefacts. Methods. The BLAST+ program was used find short reads coding neuropeptides neurohormons in publicly available read archives. Such were then similar same archives, DNA assembly Trinity employed construct contigs encoding neuropeptide precursors as completely possible. Results. seven species analyzed this fashion, crabs Eriocheir sinensis, Carcinus maenas Scylla paramamosain , shrimp Litopenaeus vannamei lobster Homarus americanus fresh water prawn Macrobrachium rosenbergii crayfish Procambarus clarkii had remarkably neuropeptidomes. some could not assembled, many cases individual pertaining missing show unambiguously that these present species. In other cases, tissues express those construction cDNA libraries. One novel identified: elongated PDH (pigment dispersing hormone), a variation has two-amino-acid insertion its core sequence. Hyrg is another peptide ubiquitously decapods likely precursor. Discussion. Many insect have lost one more genes, but apart from hyrg all at least allatotropin only decapods. This strong similarity neuropeptidomes makes it possible predict receptors for been deorphanized insects. includes androgenic insulin-like seems homologous drosophila 8.

Language: Английский

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Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans

Proceedings of the National Academy of Sciences, Journal Year: 2017, Volume and Issue: 114(20)

Published: May 1, 2017

In vertebrates thyrotropin-releasing hormone (TRH) is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating (TSH) levels as well neuromodulatory functions. However, functional equivalent in protostomian animals remains unknown, although TRH receptors are proto- and deuterostomians. Here we identify TRH-like precursor Caenorhabditis elegans belongs to bilaterian family precursors. Using CRISPR/Cas9 RNAi reverse genetics, show neuropeptides, through activation their receptor TRHR-1, promote growth Celegans peptides from pharyngeal motor neurons required for normal body size, knockdown muscle cells reduces growth. Mutants deficient signaling have no defects pumping or isthmus peristalsis rates, but defect depends on bacterial diet. addition decrease growth, trh-1 mutants reduced number offspring. Our study suggests an evolutionarily ancient neuropeptide, having its origin before divergence protostomes deuterostomes, may ancestrally been involved postembryonic reproduction.

Language: Английский

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Identification of G protein coupled receptors for opsines and neurohormones in Rhodnius prolixus. Genomic and transcriptomic analysis

Insect Biochemistry and Molecular Biology, Journal Year: 2015, Volume and Issue: 69, P. 34 - 50

Published: May 12, 2015

Language: Английский

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Transcriptomic analysis of crustacean neuropeptide signaling during the moult cycle in the green shore crab, Carcinus maenas

BMC Genomics, Journal Year: 2018, Volume and Issue: 19(1)

Published: Sept. 26, 2018

Ecdysis is an innate behaviour programme by which all arthropods moult their exoskeletons. The complex suite of interacting neuropeptides that orchestrate ecdysis well studied in insects, but details the crustacean cassette are fragmented and our understanding this process comparatively crude, preventing a meaningful evolutionary comparison. To begin to address issue we identified transcripts coding for putative receptors central nervous system (CNS) Y-organs (YO) within crab, Carcinus maenas, mapped expression profiles across accurately defined stages cycle using RNA-sequencing. We also gene epidermally-derived YO, only role synthesis ecdysteroid moulting hormones, elucidate peptides G protein-coupled (GPCRs) might have function ecdysis. Transcriptome mining CNS transcriptome yielded neuropeptide representing 47 families 66 GPCRs. Neuropeptide were differentially expressed included carcikinin, hyperglycemic hormone-2, cardioactive peptide, whilst single receptor, proctolin R1, was expressed. Carcikinin mRNA particular exhibited dramatic increases pre-moult, suggesting regulation. Crustacean hormone-2 elevated post- pre-moult regulates insect plays stereotyped motor activity during ecdysis, pre-moult. In several receptor cycle, as neuropeptide, neuroparsin-1. Whilst differential expected, discovery surprising. Analysis GPCR transcript between YO epidermis revealed 11 be upregulated thus now candidates peptide control data presented represent comprehensive survey deduced C. maenas neuropeptidome Importantly, described these staged cycles tissues key programme. This study provides important avenues future exploration functionality receptor-ligand pairs crustaceans.

Language: Английский

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Cholecystokinin/sulfakinin peptide signaling: conserved roles at the intersection between feeding, mating and aggression

Cellular and Molecular Life Sciences, Journal Year: 2022, Volume and Issue: 79(3)

Published: March 1, 2022

Neuropeptides are the most diverse messenger molecules in metazoans and involved regulation of daily physiology a wide array behaviors. Some neuropeptides their cognate receptors structurally functionally well conserved over evolution bilaterian animals. Among these peptides related to gastrin cholecystokinin (CCK). In mammals, CCK is produced by intestinal endocrine cells brain neurons, regulates gall bladder contractions, pancreatic enzyme secretion, gut functions, satiety food intake. Additionally, plays important roles neuromodulation several circuits that regulate reward, anxiety, aggression sexual behavior. invertebrates, CCK-type (sulfakinins, SKs) are, with few exceptions, neurons only. Common among invertebrates SKs mediate ingestion variety mechanisms. Also secretion digestive enzymes has been reported. Studies genetically tractable fly Drosophila have advanced our understanding SK signaling mechanisms feeding, but also gustatory sensitivity, locomotor activity, reproductive A set eight SK-expressing competing males, they integrate internal state external stimuli diminish sex drive increase aggression. The same sugar gustation, induce reduce feeding. Although functional CCK/SK appear between available data suggest underlying differ.

Language: Английский

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In silico prediction of the G-protein coupled receptors expressed during the metamorphic molt of Sagmariasus verreauxi (Crustacea: Decapoda) by mining transcriptomic data: RNA-seq to repertoire

General and Comparative Endocrinology, Journal Year: 2016, Volume and Issue: 228, P. 111 - 127

Published: Feb. 2, 2016

Language: Английский

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Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

Scientific Reports, Journal Year: 2016, Volume and Issue: 6(1)

Published: June 29, 2016

Abstract In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding and associated GPCRs rice stem borer Chilo suppressalis were identified a transcriptomic analysis used identify potential targets for disruption protection crops. Forty-three candidate found encode neuropeptide precursors all known insect except arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like 2-4 (NPLP2-4) proctolin. addition, novel alternative splicing variants three (allatostatin CC, CCHamide 1 short F) reported first time 51 putative identified. Phylogenetic analyses demonstrated that 44 these belong A-family (or rhodopsin-like), 5 B-family secretin-like) 2 leucine-rich repeat-containing GPCRs. These likely ligands also described. qRT-PCR revealed expression profiles GPCR various tissues C. . Our study provides fundamental information may further our understanding neuropeptidergic signaling systems Lepidoptera aid design peptidomimetics, pseudopeptides or small molecules capable disrupting regulated receptors.

Language: Английский

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Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle

BMC Genomics, Journal Year: 2019, Volume and Issue: 20(1)

Published: Jan. 22, 2019

G-protein coupled receptors (GPCRs) are ancient, ubiquitous, constitute the largest family of transducing cell surface proteins, and integral to communication via an array ligands/neuropeptides. Molt inhibiting hormone (MIH) is a key neuropeptide that controls growth reproduction in crustaceans by regulating molt cycle. It inhibits ecdysone biosynthesis pair endocrine glands (Y-organs; YOs) through binding yet uncharacterized GPCR, which triggers signalling cascade, leading inhibition ecdysis sequence. When MIH release stops, synthesized released hemolymph. A peak titer followed molting event. transcriptome blackback land crab Gecarcinus lateralis YOs across was utilized this study curate list GPCRs their expression order better assess involved process.Ninety-nine G. putative were obtained screening YO against Pfam database. Phylogenetic analysis classified 49 as class (Rhodopsin-like receptor), 35 B (Secretin 9 C (metabotropic glutamate). Further phylogenetic identified GPCRs, including those for Allatostatin A, B, Bursicon, CCHamide, FMRFamide, Proctolin, Corazonin, Relaxin, biogenic amine Serotonin. Three clustered with recently CHH (CHHRs), differential over cycle suggests they associated ecdysteroidogenesis regulation. Two Corazonin showed much higher compared all other suggesting important role regulation.Molting requires orchestrated regulation ecdysteroid synthesis multiple neuropeptides. In study, we curated comprehensive expressed could include receptor whose activation negatively regulates molting. Orthologs found be insects also identified, LGR3 receptor, latter at level than receptors,

Language: Английский

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