Identification and Molecular Mechanism of Novel Two-Way Immunomodulatory Peptides from Ovalbumin: In Vitro Cell Experiments, De Novo Sequencing, and Molecular Docking DOI

Zuyue Li,

Mahmoud Abou-Elsoud, Hang Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9856 - 9866

Published: April 18, 2024

The purpose of this study was to identify ovalbumin-derived immunomodulatory peptides by in vitro cell experiments, de novo sequencing, and molecular docking. Ovalbumin hydrolysates were prepared two enzymes (alkaline protease papain) individually, sequentially, or simultaneously, respectively. simultaneous enzymatic hydrolysate (OVAH) had a high degree hydrolysis (38.12 ± 0.48%) exhibited immune-enhancing anti-inflammatory activities. A total 160 identified LC–MS/MS OVAH. Three novel NVMEERKIK, ADQARELINS, WEKAFKDE bound TLR4-MD2 through hydrogen bonds hydrophobic interactions with binding affinity energies −181.40, −178.03, −168.12 kcal/mol, These three synthesized validated for two-way activity. NVMEERKIK exhibiting the strongest activity, increased NO TNF-α levels 128.69 38.01%, respectively, normal RAW264.7 cells reduced 27.31 39.13%, lipopolysaccharide-induced inflammatory cells. Overall, first revealed that ovalbumin could be used as an source controlling factor secretion.

Language: Английский

Bioactive Peptides from Edible Mushrooms—The Preparation, Mechanisms, Structure—Activity Relationships and Prospects DOI Creative Commons
Haiyan Li, Jian Gao, Fen Zhao

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(15), P. 2935 - 2935

Published: Aug. 2, 2023

Mushroom bioactive peptides (MBPs) are extracted directly or indirectly from edible mushrooms. MBPs known to have antioxidant, anti-aging, antibacterial, anti-inflammatory and anti-hypertensive properties, facilitate memory cognitive improvement, antitumour anti-diabetes activities, cholesterol reduction. exert antioxidant effects by regulating the MAPK, Keap1-Nrf2-ARE, NF-κB TNF pathways. In addition, anti-tumour stimulating proliferation of macrophages. The bioactivities closely related their molecular weights, charge, amino acid compositions sequences. Compared with animal-derived peptides, ideal raw materials for healthy functional products advantages abundance resources, safety, low price, easy-to-achieve large-scale production valuable nutrients health maintenance disease prevention. this review, preparation, bioactivities, mechanisms structure–activity relationships were described. main challenges prospects application in also discussed. This review aimed provide a comprehensive perspective MBPs.

Language: Английский

Citations

20
Plant Protein-Derived Active Peptides: A Comprehensive Review DOI
Feng Zhu,

Jiarui Cao,

Yiting Song

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(51), P. 20479 - 20499

Published: Dec. 18, 2023

Active peptides are a class of physiologically active protein fragments, which can be prepared from different sources. In the past few decades, production with various effects plant proteins continues to receive academic attention. With advances in extraction, purification, and characterization techniques, protein-derived continue discovered. They have been proven functional activities such as antioxidant, antihypertensive, immunomodulatory, antimicrobial, anti-inflammatory, antidiabetic, antithrombotic, so on. this review, we searched Web Science China National Knowledge Infrastructure for relevant articles published recent years. There 184 included manuscript. The current status is systematically introduced, including their sources, preparation, purification identification methods, physiological activities, applications food industry. Special emphasis has placed on problems peptide exploration future trend. Based these, it expected provide theoretical reference further exploitation peptides, promote healthy rapid development

Language: Английский

Citations

17
Antibacterial and Antiviral Properties of Chenopodin-Derived Synthetic Peptides DOI Creative Commons

Marcia L. Feijoo-Coronel,

Bruno Mendes, David Ramírez

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(1), P. 78 - 78

Published: Jan. 14, 2024

Antimicrobial peptides have been developed based on plant-derived molecular scaffolds for the treatment of infectious diseases. Chenopodin is an abundant seed storage protein in quinoa, Andean plant with high nutritional and therapeutic properties. Here, we used computer- physicochemical-based strategies designed four derived from primary structure Chenopodin. Two reproduce natural fragments 14 amino acids Chenopodin, named Chen1 Chen2, two engineered same length were sequence. The containing amide side chains replaced by arginine (ChenR) or tryptophan (ChenW) to generate cationic hydrophobic peptides. evaluation these 14-mer

Language: Английский

Citations

8
Bioactive Peptides from Fermented Foods: Production Approaches, Sources, and Potential Health Benefits DOI Creative Commons
Lucilla Fabbri, Andrea Cavallero, Francesco Vidotto

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(21), P. 3369 - 3369

Published: Oct. 23, 2024

Microbial fermentation is a well-known strategy for enhancing the nutraceutical attributes of foods. Among outcomes, bioactive peptides (BAPs), short chains amino acids resulting from proteolytic activity, are emerging as promising components thanks to their bioactivities. Indeed, BAPs offer numerous health benefits, including antimicrobial, antioxidant, antihypertensive, and anti-inflammatory properties. This review focuses on production during process, emphasizing how different microbial strains conditions influence quantity quality these peptides. Furthermore, it examines benefits fermented foods, highlighting potential in disease prevention overall promotion. Additionally, this addresses challenges future directions field. comprehensive overview underscores promise foods sustainable potent sources peptides, with significant implications developing functional nutraceuticals.

Language: Английский

Citations

8
Exploring Immune Redox Modulation in Bacterial Infections: Insights into Thioredoxin-Mediated Interactions and Implications for Understanding Host–Pathogen Dynamics DOI Creative Commons
Omer M. A. Dagah,

Billton Bryson Silaa,

Minghui Zhu

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 545 - 545

Published: April 29, 2024

Bacterial infections trigger a multifaceted interplay between inflammatory mediators and redox regulation. Recently, accumulating evidence has shown that signaling plays significant role in immune initiation subsequent cell functions. This review addresses the crucial of thioredoxin (Trx) system reactions regulation responses during bacterial infections. Downstream pathways various cells involve thiol-dependent regulation, highlighting pivotal roles thiol systems defense mechanisms. Conversely, survival virulence pathogenic bacteria are enhanced by their ability to counteract oxidative stress attacks. is achieved through reduction oxidized proteins modulation redox-sensitive pathways, which functions Trx system, thereby fortifying resistance. Moreover, some selenium/sulfur-containing compounds could potentially be developed into targeted therapeutic interventions for bacteria. Taken together, key player infection, contributes host–pathogen interactions, offering valuable insights future research development.

Language: Английский

Citations

7
Bioactive peptides from food science to pharmaceutical industries: their mechanism of action, potential role in cancer treatment and available resources DOI Creative Commons

Maryam Bidram,

Mohamad Reza Ganjalikhany

Heliyon, Journal Year: 2024, Volume and Issue: 10(23), P. e40563 - e40563

Published: Nov. 22, 2024

Language: Английский

Citations

6
Biological activities of peptides obtained by pepsin hydrolysis of fishery products DOI
Daniel Castañeda‐Valbuena,

Ángel Berenguer-Murcia,

Roberto Fernandéz‐Lafuente

et al.

Process Biochemistry, Journal Year: 2022, Volume and Issue: 120, P. 53 - 63

Published: June 3, 2022

Language: Английский

Citations

27
Bioactive peptides in fermented foods and their application: a critical review DOI Open Access
Rounak Chourasia, Loreni Chiring Phukon, Md Minhajul Abedin

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2022, Volume and Issue: 3(1), P. 88 - 109

Published: Sept. 1, 2022

Language: Английский

Citations

25
MPMABP: A CNN and Bi-LSTM-Based Method for Predicting Multi-Activities of Bioactive Peptides DOI Creative Commons
You Li, Xueyong Li,

Yuewu Liu

et al.

Pharmaceuticals, Journal Year: 2022, Volume and Issue: 15(6), P. 707 - 707

Published: June 3, 2022

Bioactive peptides are typically small functional with 2-20 amino acid residues and play versatile roles in metabolic biological processes. multi-functional, so it is vastly challenging to accurately detect all their functions simultaneously. We proposed a convolution neural network (CNN) bi-directional long short-term memory (Bi-LSTM)-based deep learning method (called MPMABP) for recognizing multi-activities of bioactive peptides. The MPMABP stacked five CNNs at different scales, used the residual preserve information from loss. empirical results showed that superior state-of-the-art methods. Analysis on distribution acids indicated lysine preferred appear anti-cancer peptide, leucine anti-diabetic proline anti-hypertensive peptide. analysis beneficial recognize

Language: Английский

Citations

24
Teleost Piscidins—In Silico Perspective of Natural Peptide Antibiotics from Marine Sources DOI Creative Commons
Patricia Asensio-Calavia, Sergio González-Acosta, Andrea Otazo-Pérez

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(5), P. 855 - 855

Published: May 5, 2023

Fish, like all other animals, are exposed to constant contact with microbes, both on their skin and the surfaces of respiratory digestive systems. Fish have a system non-specific immune responses that provides them initial protection against infection allows survive under normal conditions despite presence these potential invaders. However, fish less protected invading diseases than marine vertebrates because epidermal surface, composed primarily living cells, lacks keratinized serves as an efficient natural barrier in vertebrates. Antimicrobial peptides (AMPs) one type innate present life forms. AMPs been shown broader range biological effects conventional antibiotics, including antibacterial, antiviral, antiprotozoal, antifungal effects. Although AMPs, such defensins hepcidins, found relatively well conserved, piscidins exclusively Teleost not any animal. Therefore, there is information expression bioactivity AMPs. Piscidins highly effective Gram-positive Gram-negative bacteria cause disease humans be used pharmacological anti-infectives biomedicine aquaculture. To better understand benefits limitations using therapeutic agents, we conducting comprehensive study included “reviewed” category UniProt database bioinformatics tools. They amphipathic alpha-helical structures. The architecture piscidin positively charged residues influence antibacterial activity. These alpha-helices intriguing antimicrobial drugs due stability high-salt metal environments. New treatments for multidrug-resistant bacteria, cancer, inflammation may inspired by peptides.

Language: Английский

Citations

14