Advances in Engineered Macrophages: A New Frontier in Cancer Immunotherapy

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(4)

Published: April 1, 2024

Abstract Macrophages, as pivotal cells within the tumour microenvironment, significantly influence impact of and reactions to treatments for solid tumours. The rapid evolution bioengineering technology has revealed vast potential engineered macrophages in immunotherapy, disease diagnosis, tissue engineering. Given this landscape, goal harnessing innovating a novel strategy immunotherapy cannot be overstated. diverse strategies realm cancer encompassing macrophage drug delivery systems, chimeric antigen receptor therapy, synergistic treatment approaches involving bacterial outer membrane vesicles macrophages, are meticulously examined review. These methodologies designed enhance therapeutic efficacy against tumours, particularly those that drug-resistant metastatic. Collectively, these immunotherapies poised supplement refine current paradigms, thus heralding new frontier fight malignant

Language: Английский

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Bee Pollen Phytochemicals and Nutrients as Unequaled Pool of Epigenetic Regulators: Implications for Age-Related Diseases

Foods, Journal Year: 2025, Volume and Issue: 14(3), P. 347 - 347

Published: Jan. 21, 2025

Bee pollen is characterized by an exceptional diversity and abundance of micronutrients bioactive phytochemicals. This richness remains very sparsely investigated, but accumulating evidence strongly supports a promising future for bee in human nutrition medicine. Epigenetic regulation among the most compelling biomedical topics that remain completely untapped derivative research. In our current research, we identified numerous ubiquitous compounds are consistently present this matrix, regardless its botanical geographical origins, have been well studied documented as epigenetic regulators recent years. Given relative newness both research studies within nutritional, pharmaceutical, medical sciences, review aims to bridge these valuable fields advance related experimental investigations. To best knowledge, first work has aimed comprehensively investigate modulatory potential compounds. Our findings also unveiled several intriguing phenomena, such dual effect same compound depending on cellular context or some cross-generational heritability traits. Although whole extract still lacking, study clearly indicates avenue worth further We hope constitutes foundational cornerstone investigations

Language: Английский

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Macrophages and tumor-associated macrophages in the senescent microenvironment: From immunosuppressive TME to targeted tumor therapy

Pharmacological Research, Journal Year: 2024, Volume and Issue: 204, P. 107198 - 107198

Published: April 30, 2024

In-depth studies of the tumor microenvironment (TME) have helped to elucidate its cancer-promoting mechanisms and inherent characteristics. Cellular senescence, which acts as a response injury can release senescence-associated secretory phenotypes (SASPs). These SASPs various cytokines, chemokines, growth factors, remodeling TME. This continual development senescent environment could be associated with chronic inflammation immunosuppressive Additionally, influence phenotype function macrophages, leading recruitment tumor-associated macrophages (TAMs). contributes proliferation metastasis in microenvironment, working tandem immune regulation, angiogenesis, therapeutic resistance. comprehensive review covers evolving nature TAMs development. We also explored links between inflammation, TME, cellular macrophages. Moreover, we compiled tumor-specific treatment strategies centered on senescence current challenges research. study aimed clarify mechanism progression advance targeted therapies.

Language: Английский

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Exosome-shuttled miR-150–5p from LPS-preconditioned mesenchymal stem cells down-regulate PI3K/Akt/mTOR pathway via Irs1 to enhance M2 macrophage polarization and confer protection against sepsis

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 18, 2024

Rationale Sepsis is a life-threatening organ dysfunction and lack of effective measures in the current. Exosomes from mesenchymal stem cells (MSCs) reported to alleviate inflammation during sepsis, preconditioning MSCs could enhance their paracrine potential. Therefore, this study investigated whether exosomes secreted by lipopolysaccharide (LPS)-pretreated exert superior antiseptic effects, explored underlying molecular mechanisms. Methods were isolated characterized supernatants MSCs. The therapeutic efficacy normal (Exo) LPS-pretreated (LPS-Exo) evaluated terms survival rates, inflammatory response, damage an LPS-induced sepsis model. Macrophages stimulated with LPS treated Exo or LPS-Exo confirm results vivo studies, explain potential Results shown inhibit aberrant pro-inflammatory cytokines, prevent damages, improve rates septic mice greater extent than Exo. In vitro , significantly promoted M2 polarization macrophages exposed inflammation. miRNA sequencing qRT-PCR analysis identified remarkable expression miR-150–5p compared that Exo, exosomal was transferred into recipient mediated macrophage polarization. Further investigation demonstrated targets Irs1 subsequently modulates plasticity down-regulating PI3K/Akt/mTOR pathway. Conclusion current findings highly suggest derived pre-conditioned represent promising cell-free method highlight as novel target for regulating immune hyperactivation sepsis.

Language: Английский

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Tissue macrophages: origin, heterogenity, biological functions, diseases and therapeutic targets

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 7, 2025

Abstract Macrophages are immune cells belonging to the mononuclear phagocyte system. They play crucial roles in defense, surveillance, and homeostasis. This review systematically discusses types of hematopoietic progenitors that give rise macrophages, including primitive progenitors, erythro-myeloid stem cells. These have distinct genetic backgrounds developmental processes. Accordingly, macrophages exhibit complex diverse functions body, phagocytosis clearance cellular debris, antigen presentation, response, regulation inflammation cytokine production, tissue remodeling repair, multi-level regulatory signaling pathways/crosstalk involved homeostasis physiology. Besides, tumor-associated a key component TME, exhibiting both anti-tumor pro-tumor properties. Furthermore, functional status is closely linked development various diseases, cancer, autoimmune disorders, cardiovascular disease, neurodegenerative metabolic conditions, trauma. Targeting has emerged as promising therapeutic strategy these contexts. Clinical trials macrophage-based targeted drugs, immunotherapies, nanoparticle-based therapy were comprehensively summarized. Potential challenges future directions targeting also been discussed. Overall, our highlights significance this versatile cell human health which expected inform research clinical practice.

Language: Английский

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Simvastatin-Loaded Polymeric Nanoparticles: Targeting Inflammatory Macrophages for Local Adipose Tissue Browning in Obesity Treatment

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 29, 2024

Obesity is defined as chronic, low-grade inflammation within specific tissues. Given the escalating prevalence of obesity among individuals all ages, has reached epidemic proportions, posing an important public health challenge. Despite significant advancements in treating obesity, conventional approaches remain largely ineffective or involve severe side effects, thus underscoring pressing need to explore and develop treatment approaches. Targeted local immunomodulation using nanoparticles (NPs) can influence fat production utilization processes. Statins, known for their anti-inflammatory properties, show potential mitigating obesity-related inflammation. A localized delivery option offers several advantages over oral parenteral methods. Here, we developed simvastatin (Sim) encapsulated PLGA NPs (Sim-NP) Sim adipose tissues (ATs) treat obesity. In vitro experiments revealed strong effects Sim-NPs, which resulted enhanced modulation macrophage (MΦ) polarization induction AT browning. We then extended our investigation

Language: Английский

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Prediction of ADMET profile and anti-inflammatory potential of chamuangone

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 23, 2025

Chamuangone, a compound extracted from the leaves of Garcinia cowa, exhibits various biological activities. Yet, its absorption, distribution, metabolism, excretion, and toxicity (ADMET) profile as well anti-inflammatory effects in macrophages remain unexplored. In this study, we employed computational tool to predict ADMET chamuangone performed molecular docking simulations assess interactions with key proteins involved inflammatory pathways. Additionally, vitro experiments using lipopolysaccharide (LPS)-induced RAW264.7 were evaluate effects. The present findings suggest that has favorable properties, interacts strongly major inflammation, activity LPS-induced macrophages, making it promising agent.

Language: Английский

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Toll-Like Receptors in Kidney Ischemia-Reperfusion Injury: Modulating Macrophage Responses for Therapeutic Insights

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155940 - 155940

Published: March 1, 2025

Language: Английский

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Construction of artificial lung tissue structure with 3D-inkjet bioprinting core for pulmonary disease evaluation

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: March 1, 2025

By integrating 3D-inkjet bioprinting technology, differentiated human cells can be assembled into artificial lung tissue structure to achieve a rapid, efficient, and reproducible disease model construction process. Here, we developed novel bioprinting-based method construct (ALTs) for acute injury (ALI) modeling, research application. It also used study the role of relevant in by adjusting cell type adapted bio-functions immune during cell-cell interactions. Firstly, series process optimizations were done mass-produce alginate hydrogel microspheres (Alg) with particle size 262.63 ± 5 μm using 3D bioprinter, then I collagen polydopamine deposited turns adhesion layer on surfaces Alg (P-Alg) was increased 328.41 3.81 μm. This platform exhibites good stability, timescale-dependent behavior, long-term adhesion. Subsequently, several including endothelial, epithelial, fibroblast, even such as macrophages adhered P-Alg through rotational culture, leading contractions aggregation, subsequently formed ALTs or (ALTs@M) alveolar-like structure. Finally, successfully constructed an ALI barrier damage lipopolysaccharide stimulation vitro, comparison secreted inflammatory factors between ALTs@M. Results demonstrated that ALTs@M more effective than stimulating microenvironment lungs, providing vitro cellular interactions macrophage research. Altogether, this strategy technology allowed flexible development structures potential models preclinical studies.

Language: Английский

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An effective approach to modulate mitochondrial function in murine primary macrophages by a mitochondria-targeted nanocapsule, MITO-Porter

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118019 - 118019

Published: April 6, 2025

Language: Английский

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