Xanthohumol ameliorates drug-induced hepatic ferroptosis via activating Nrf2/xCT/GPX4 signaling pathway

Phytomedicine, Journal Year: 2024, Volume and Issue: 126, P. 155458 - 155458

Published: Feb. 22, 2024

Language: Английский

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Sodium aescinate-induced hepatotoxicity via ATF4/GSH/GPX4 axis-mediated ferroptosis

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 7, 2025

Sodium aescinate (SA), a natural plant extract with various bioactivities, is widely used to treat oedema and inflammation in clinics. However, adverse events, including liver injury, kidney phlebitis, have been reported patients SA recent years. In this study, we BALB/c mice L02 cells evaluate the role of ferroptosis SA-induced injury. significantly increased AST, ALT, MDA Fe2+, decreased GSH levels, induced pathological changes vivo. also reduced viability LDH release, intracellular cysteine reduction, depletion, iron accumulation, ROS production, lipid peroxidation, indicating that causes ferroptosis. addition, inhibited transcriptional activity activating transcription factor 4 (ATF4) subsequently expression downstream genes xCT (solute carrier family 7a member 11, SLC7A11) Cystathionine gamma-lyase (CTH) which play vital roles biosynthesis. Interestingly, cytotoxic effects were effectively attenuated by ATF4 overexpression, while they aggravated silencing. These results revealed triggers hepatocyte inhibiting ATF4, an oxidative imbalance.

Language: Английский

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Sakuranetin Prevents Acetaminophen-Induced Liver Injury via Nrf2-Induced Inhibition of Hepatocyte Ferroptosis

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 159 - 171

Published: Jan. 1, 2025

Oxidative stress is an important cause of acetaminophen (APAP)-induced liver injury (AILI). Sakuranetin (Sak) antitoxin from the cherry flavonoid plant with good antioxidant effects. However, whether sakuranetine has a protective effect on APAP-induced not clear. Mouse and HepG2 cell models APAP were used to investigate sakuranetin AILI its mechanism. Serum transaminase levels, histological changes, inflammatory mediators, oxidative stress, ferroptosis-related markers Nrf2 signaling pathway proteins analyzed. significantly reduced serum alanine aminotransferase (ALT) aspartate (AST), as well factor; increased activity decreased death; inhibited ROS production, glutathione (GSH) content, expression Glutathione Peroxidase 4 (GPX4) Solute Carrier Family 7 Member 11 (SLC7A11), malondialdehyde Acyl-CoA Synthetase Long Chain (ACSL4) in mice cells after treatment. Further analysis showed that induced activation NFE2 Like BZIP Transcription Factor 2 (Nrf2) tissue promoted nuclear translocation Nrf2. Moreover, hepatoprotective inhibitory ferroptosis attenuated by inhibitor ML385. alleviates activating inhibiting ferroptosis, may be potential therapeutic agent for treatment AILI.

Language: Английский

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Picein alleviates oxidative stress and promotes bone regeneration in osteoporotic bone defect by inhibiting ferroptosis via Nrf2/HO‐1/GPX4 pathway

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(7), P. 4066 - 4085

Published: May 10, 2024

Abstract Osteoporosis (OP) can result in slower bone regeneration than the normal condition due to abnormal oxidative stress and high levels of reactive oxygen species (ROS), a detrimental for formation, making OP‐related healing significant clinical challenge. As osteogenic differentiation ability marrow mesenchymal stem cells (BMSCs) is closely related regeneration; currently, this study assessed effects Picein on BMSCs vitro osteoporotic defect vivo. Cell viability was determined by CCK‐8 assay. The production malonaldehyde, superoxide dismutase activities, glutathione evaluated using commercially available kits, flow cytometry analysis adopted detect macrophage polarization. Osteogenic capacity alkaline phosphatase (ALP) activity, ALP staining, Alizarin red S staining. expression osteogenic‐related proteins (OPN, Runx‐2, OCN) genes (ALP, BMP‐4, COL‐1, Osterix) were Western blotting real‐time PCR (RT‐PCR). In addition, proliferation, migration ability, angiogenic human umbilical vein endothelial (HUVECs) EdU scratch test, transwell assay, tube formation respectively. Angiogenic‐related (VEGF, vWF, CD31) also RT‐PCR. Results showed that alleviated erastin‐induced stress, enhanced BMSCs, angiogenesis HUVECs, protects against ferroptosis through Nrf2/HO‐1/GPX4 axis. Moreover, regulate immune microenvironment promoting polarization M2 macrophages vitro. reduce inflammation promotes OP rat models Altogether, these results suggested promote alleviate via pathway, offering as novel antioxidant agent treating defect.

Language: Английский

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Protective effect of the total flavonoids from Clinopodium chinense against LPS-induced mice endometritis by inhibiting NLRP3 inflammasome-mediated pyroptosis

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 312, P. 116489 - 116489

Published: April 11, 2023

Language: Английский

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Total saponins from Panax japonicus attenuate acute alcoholic liver oxidative stress and hepatosteatosis by p62-related Nrf2 pathway and AMPK-ACC/PPARα axis in vivo and in vitro

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 317, P. 116785 - 116785

Published: June 15, 2023

Language: Английский

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Punicalagin attenuates TNF-α-induced oxidative damage and promotes osteogenic differentiation of bone mesenchymal stem cells by activating the Nrf2/HO-1 pathway

Experimental Cell Research, Journal Year: 2023, Volume and Issue: 430(1), P. 113717 - 113717

Published: July 8, 2023

Oxidative stress is one of the most important factors in changing bone homeostasis. Redox homeostasis plays a key role osteogenic differentiation mesenchymal stem cells (BMSCs) and angiogenesis ability human umbilical vein endothelial (HUVECs) for regeneration. Currently, this study assessed effects punicalagin (PUN) on BMSCs HUVECs. Cell viability was determined by CCK-8 assay. A flow cytometry analysis adopted to detect macrophage polarization. The production reactive oxygen (ROS), glutathione (GSH), malonaldehyde (MDA) superoxide dismutase (SOD) activities were evaluated using commercially-available kits. Osteogenic capacity ALP activity, staining ARS staining. expression osteogenic-related proteins (OCN, Runx-2, OPN) Nrf/HO-1 levles Western blotting. Osteogenic-related genes (Osterix, COL-1, BMP-4, ALP) RT-PCR. Migration invasion HUVECs wound healing assay Transwell Angiogenic detected tube formation angiogenic-related (VEGF, vWF, CD31) Results showed that PUN alleviated oxidative TNF-α, enhanced Moreover, regulate immune microenvironment promoting polarization M2 macrophages reduce related products activating Nrf2/HO-1 pathway. Altogether, these results suggested can promote BMSCs, HUVECs, alleviate via pathway, offering as novel antioxidant agent treating loss diseases.

Language: Английский

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Inhibition of mitochondrial ROS-mediated necroptosis by Dendrobium nobile Lindl. alkaloids in carbon tetrachloride induced acute liver injury

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118253 - 118253

Published: April 26, 2024

Language: Английский

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Bioactive compound schaftoside from Clinacanthus nutans attenuates acute liver injury by inhibiting ferroptosis through activation the Nrf2/GPX4 pathway

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 328, P. 118135 - 118135

Published: March 30, 2024

Language: Английский

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Wogonin Mitigates Acetaminophen-Induced Liver Injury in Mice through Inhibition of the PI3K/AKT Signaling Pathway

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 332, P. 118364 - 118364

Published: May 18, 2024

Scutellaria baicalensis Georgi (SBG), a widely used traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties. Wogonin is one of the primary bioactive components SBG. Acetaminophen (APAP)-induced liver injury (AILI) represents prevalent form drug-induced damage primarily driven by inflammatory responses oxidative stress.

Language: Английский

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