Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)
Published: March 5, 2024
, an aquatic pathogenic bacterium, has been found to infect many fish species and cause huge aquaculture losses. Antibiotics are the most common drugs used treat these infections. However, antibiotic abuse can lead development of resistance. Probiotics have potential replace antibiotics for preventing Zebrafish (
Language: Английский
Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 173, P. 116418 - 116418
Published: March 9, 2024
Quercetin is a representative flavonoid that widely present in fruits, herbs, and vegetables. It also an important active core component traditional Chinese medicines. As flavonoid, quercetin has various properties exerts antioxidant, anti-inflammatory, cardioprotective effects. The public interest increasing, been used to prevent or treat numerous of diseases, such as polycystic ovary syndrome (PCOS), cancer, autoimmune diseases chronic cardiovascular clinical experiments animal studies due its powerful antioxidant minimal side marked pharmacological effects on gynecological disorders; however, there have no reviews about the potential health benefits context disorders, including PCOS, premature failure (POF), endometriosis (EM), ovarian cancer (OC), cervical (CC) endometrial carcinoma (EC). Thus, this review aimed summarize biological disorders mechanisms.
Language: Английский
Citations
7
Journal of Ovarian Research, Journal Year: 2024, Volume and Issue: 17(1)
Published: April 27, 2024
Abstract Objective The present study aimed to elucidate how mesenchymal stem cells (MSCs) application could efficiently attenuate pathological changes of letrozole-induced poly cystic ovary syndrome (PCOS) by modulating mitochondrial dynamic via PI3K-AKT pathway. Methods Thirty-two female rats were randomly divided into four experimental groups: Sham, PCOS, PCOS + MSCs, and MSCs LY294002. Sham group received 0.5% w/v carboxymethyl cellulose (CMC); the letrozole (1 mg/kg, daily) in CMC for 21 days. Animals 1 × 10 6 MSCs/rat (i.p,) on 22th day study. In LY294002 group, (PI3K-AKT inhibitor) 40 min before MSC transplantation. Mitochondrial gene expression, membrane potential (MMP), citrate synthase (CS) activity, oxidative stress, inflammation, ovarian histological parameters, serum hormone levels, homeostatic model assessment insulin resistance (HOMA-IR), glucose concentrations, p-PI3K p-AKT protein levels evaluated at end experiment. Results showed a significant disruption dynamics changes, lower MMP, CS, super oxide dismutase (SOD) estrogen level. They also had notable rise HOMA-IR, testosterone level, tumor necrosis factor-α (TNF-α) interleukin-6 (IL-6) malondialdehyde (MDA) content as well decrease compared group. transplantation improve above parameters. Administration pathway deteriorated markers, stress status, inflammation hormonal glucose, follicular development Conclusions This demonstrated that protective effects regulating dynamics, promoting biogenesis, competing with redox status response mainly mediated through model.
Language: Английский
Citations
6
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 9, 2025
As one of the essential lignan derivative found in traditional Chinese medicinal herbs, secoisolariciresinol diglucoside (SDG) was proved to promote women's health through its phytoestrogenic properties. Increasingly studies indicated that this compound could be a potential drug capable preventing estrogen-related diseases. Here, we aimed investigate whether SDG can counteract cyclophosphamide (CTX) induced premature ovarian insufficiency (POI) and further explore specific molecular mechanism. In study, first validated therapeutic effect on POI mouse model. Then, mechanism by which improves is predicted combination network pharmacology, authenticity confirmed experimental verification, docking analysis dynamics simulation. The results showed significantly alleviated improving indices follicle counts while protecting against CTX-induced damage modulating PI3K/Akt signaling pathway KGN cells. addition, SDG's high affinity for Akt1 PI3Kγ, pinpointing precise interaction sites. These underscore protective mechanisms damage, highlighting potential. summary, our study identified ameliorate with action intricately linked modulation pathway.
Language: Английский
Citations
0
Life Sciences, Journal Year: 2025, Volume and Issue: 364, P. 123417 - 123417
Published: Jan. 27, 2025
Language: Английский
Citations
0
Open Medicine, Journal Year: 2025, Volume and Issue: 20(1)
Published: Jan. 1, 2025
Abstract Background and objective Early nutritional support holds paramount importance for postoperative gastric cancer (GC) patients. Peptidase M20 domain containing 1 (PM20D1) is a secretory enzyme associated with glucose lipid metabolism. However, there dearth of clinical studies delving into the connection between PM20D1, metabolism, inflammatory factors in GC patients who have received enteral nutrition (EN). This research aimed to investigate serum levels PM20D1 following early EN their potential associations markers, factors. Methods prospective observational study enrolled 180 May 2020 July 2022. On first day, all support, which was maintained duration 5 days. Serum interleukin (IL)-6, IL-1β, C-reactive protein were measured on sixth day after surgery using an enzyme-linked immunosorbent assay. Data demographics, statistics, parameters, prognostic index (PNI) collected. Patients followed up 12 months, both overall survival disease-free recorded. Results In low PNI group, albumin (ALB), blood lymphocytes (BL) showed significant reductions. Pearson analysis revealed negative correlation IL-6 levels, whereas positive emerged ALB BL levels. Furthermore, demonstrated as biomarker diagnosing poor status (PNI < 43) risk factor Conclusion remarkably declined status.
Language: Английский
Citations
0
Revista Fitos, Journal Year: 2025, Volume and Issue: 19(1)
Published: Feb. 1, 2025
Citations
0
The Journal of Steroid Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106767 - 106767
Published: April 1, 2025
Language: Английский
Citations
0
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(10), P. e0311978 - e0311978
Published: Oct. 23, 2024
Primordial follicle activation (PFA) is a pivotal event in female reproductive biology, coordinating the transition from quiescent to growing follicles. This study employed comprehensive single-cell RNA sequencing gain insights into detailed regulatory mechanisms governing synchronized dormancy and between granulosa cells (GCs) oocytes with progression of PFA process. Wntless ( Wls ) conditional knockout (cKO) mice served as unique model, suppressing pre-GCs GCs, disrupting somatic cell-derived WNT signaling ovary. Our data revealed immediate transcriptomic changes GCs post-PFA cKO mice, leading divergent trajectory, while exhibited modest alterations. Subpopulation analysis identified molecular pathways affected by on GC maturation, along specific gene signatures linked dormant activated oocytes. Despite minimal evidence continuous up-regulation dormancy-related genes oocytes, loss (pre-)GCs impacted expression even before PFA, subsequently influencing them globally. The infertility observed was attributed compromised GC-oocyte crosstalk microenvironment for highlights role WNT-signaling pathway its signature, emphasizing importance intercellular orchestrating folliculogenesis.
Language: Английский
Citations
2
Discovery Medicine, Journal Year: 2024, Volume and Issue: 36(184), P. 946 - 946
Published: Jan. 1, 2024
Polycystic ovary syndrome (PCOS) commonly impacts fertile females with potentially severe effects on fertility and metabolism. Blood ghrelin levels are lower in PCOS patients, exogenous supplements have been proposed for their potential to trigger anti-inflammatory at the cellular level. This study aimed investigate whether pretreatment reduced inflammation, insulin resistance, reproductive abnormalities underlying mechanism of this disorder.
Language: Английский
Citations
1
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 116093 - 116093
Published: Dec. 29, 2023
Polycystic ovary syndrome (PCOS) is a highly prevalent endocrine and metabolic disorder that closely associated with the proliferation apoptosis of ovarian granulosa cells (GCs). Ampelopsis japonica (AJ) dried tuberous root (Thunb.) Makino (A. japonica), anti-inflammatory, antioxidant, antibacterial, antiviral, wound-healing, antitumor properties; however, it unclear whether this herb has therapeutic effect on PCOS. Therefore, study aimed to investigate pharmacological AJ PCOS reveal its potential mechanism action. A rat model was established using letrozole. After establishing model, rats received oral treatment Diane-35 (Positive drug: ethinylestradiol + cyproterone tablets) for 2 weeks. Lipidomics conducted liquid-phase mass spectrometry chromatography. significantly regulated serum hormone levels attenuated pathological variants in ovaries Furthermore, reduced apoptotic rate GCs. Lipidomic analysis revealed modulated glycerolipid glycerophospholipid pathways mediated by lipoprotein lipase (Lpl), diacylglycerol choline phosphotransferase (Chpt1), choline/ethanolamine (Cept1). we may reduce GC modulating lipid metabolism, ultimately improving ovulatory dysfunction novel candidate treatment.
Language: Английский
Citations
2