Midbrain node for context-specific vocalisation in fish

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Vocalizations communicate information indicative of behavioural state across divergent social contexts. Yet, how brain regions actively pattern the acoustic features context-specific vocal signals remains largely unexplored. The midbrain periaqueductal gray (PAG) is a major site for initiating vocalization among mammals, including primates. We show that PAG neurons in highly fish species ( Porichthys notatus ) are activated distinct patterns during agonistic versus courtship calling by males, with few co-activated non-vocal behaviour, foraging. Pharmacological manipulations within vocally active PAG, but not hindbrain, sites evoke network output to sonic muscles matching temporal and calls, showing balance inhibitory excitatory dynamics likely necessary patterning different call types. Collectively, these findings support hypothesis mammals share functionally comparable nodes some can influence structure signals.

Language: Английский

---

Localization and divergent profiles of estrogen receptors and aromatase in the vocal and auditory networks of a fish with alternative mating tactics

The Journal of Comparative Neurology, Journal Year: 2013, Volume and Issue: 521(12), P. 2850 - 2869

Published: March 5, 2013

Estrogens play a salient role in the development and maintenance of both male female nervous systems behaviors. The plainfin midshipman (Porichthys notatus), teleost fish, has two reproductive morphs that follow alternative mating tactics diverge multiple somatic, hormonal, neural traits, including central control morph-specific vocal After we identified duplicate estrogen receptors (ERβ1 ERβ2) midshipman, developed antibodies to localize protein expression vocal-acoustic networks saccule, auditory division inner ear. As other species, ERβ1 ERβ2 were robustly expressed telencephalon hypothalamus brain regions shown previously exhibit strong ERα aromatase (estrogen synthetase, CYP19) midshipman. Like aromatase, label colocalized with glial fibrillary acidic (GFAP) telencephalic radial cells. Quantitative polymerase chain reaction revealed similar patterns transcript abundance across for ERβ1, ERβ2, ERα, forebrain saccule. In contrast, ERs varied significantly between around sexually polymorphic motor nucleus (VMN). Together, results suggest VMN is major target within estrogen-sensitive hindbrain network directly determines duration, frequency, amplitude vocalizations. Comparable regional differences steroid receptor abundances likely regulate behaviors males females species exhibiting tactics.

Language: Английский

---

Catecholaminergic connectivity to the inner ear, central auditory, and vocal motor circuitry in the plainfin midshipman fish porichthys notatus

The Journal of Comparative Neurology, Journal Year: 2014, Volume and Issue: 522(13), P. 2887 - 2927

Published: April 8, 2014

Although the neuroanatomical distribution of catecholaminergic (CA) neurons has been well documented across all vertebrate classes, few studies have examined CA connectivity to physiologically and anatomically identified neural circuitry that controls behavior. The goal this study was characterize in brain inner ear plainfin midshipman fish (Porichthys notatus) with particular emphasis on their relationship labeled both produces encodes social acoustic signals species. Neurobiotin labeling main auditory end organ, saccule, combined tyrosine hydroxylase immunofluorescence (TH-ir) revealed a strong innervation peripheral central system. Diencephalic TH-ir periventricular posterior tuberculum, known be dopaminergic, send ascending projections ventral telencephalon prominent descending vocal-acoustic integration sites, notably hindbrain octavolateralis efferent nucleus, as onto base hair cells saccule via nerve VIII. backfills vocal combination terminals components pattern generator, which appears largely originate from local but may include input diencephalic well. This provides evidence catecholamines are important modulators acoustic-driven behavior fish. demonstration organ hearing nonmammalian suggests conserved anatomical functional role for dopamine normal audition.

Language: Английский

---

Distinct patterns of activity within columns of the periaqueductal gray are associated with functionally distinct birdsongs

Annals of the New York Academy of Sciences, Journal Year: 2023, Volume and Issue: 1530(1), P. 161 - 181

Published: Oct. 6, 2023

Abstract Male songbirds produce female‐directed songs in spring that convey a state of sexual motivation. Many also sing fall flocks affiliative/gregarious contexts which song is linked to an intrinsic positive affective state. The periaqueductal gray (PAG) mammals, organized into functional columns, integrates information from multiple brain regions and relays this vocal motor areas so animal emits signal reflective its Here, we test the hypothesis distinct columns songbird PAG play roles states communicated by sexually motivated gregarious song. We quantified numbers immediate early gene ZENK‐positive cells 16 subregions male European starlings ( Sturnus vulgaris ) after singing or Results suggest context‐specifically regulate song, agonistic, courtship behaviors. A second exploratory, tract‐tracing study demonstrated inputs specific medial preoptic nucleus may contribute behaviors indicative social dominance. Together, findings conserved role context‐specific other

Language: Английский

---

Emerging trends on nanomaterial-based simultaneous electrochemical sensing of dopamine and acetaminophen

Results in Chemistry, Journal Year: 2024, Volume and Issue: 7, P. 101489 - 101489

Published: Jan. 1, 2024

Recent years have witnessed significant developments in electrochemical sensing, especially voltammetric techniques, which are known for providing flexible platforms the simultaneous measurement of analgesic acetaminophen (AC) and neurotransmitter dopamine (DA). The most recent advancements trends methods detection these two analytes thoroughly reviewed this paper. different novel electrode materials, viz. nanostructures, several surface modifications used to improve sensitivity, selectivity, stability sensors that target electrochemistry dopamine, been carried out studies published last decade. A wide range techniques covered investigation, such as square wave voltammetry, differential pulse cyclic voltammetry. review also critically examines drawbacks restrictions existing approaches, from problems with selectivity intricate matrices requirement in-vivo applications real-time monitoring terms diagnostics. pursuit multifunctional sensors, integration advanced nanomaterials, standardization experimental conditions identified critical research gaps. study concludes some notions about potential future developments, highlighting possible influence current on advancement acetaminophen. It suggests directions ongoing how exploration unexplored areas can be attended develop highly sensitive, selective, cost-effective sensors.

Language: Английский

---

Dehydroepiandrosterone (DHEA) increases undirected singing behavior and alters dopaminergic regulation of undirected song in non-breeding male European starlings (Sturnus vulgaris)

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: May 10, 2023

Introduction It has been proposed that in species defend territories across multiple life history stages, brain metabolism of adrenal dehydroepiandrosterone (DHEA) regulates aggressive behavior at times when gonadal androgen synthesis is low (i.e. the non-breeding season). To date, a role for DHEA regulation other forms social are expressed outside context breeding remains unknown. Methods In this experiment, we used European starling ( Sturnus vulgaris ) model system to investigate neuroendocrine singing by males condition. Starling song spontaneous, not directed towards conspecifics, and functions maintain cohesion overwintering flocks. Results Using within-subjects design, found implants significantly increase undirected condition male starlings. Given known modulate neurotransmitter systems including dopamine (DA) DA song, subsequently immunohistochemistry phosphorylated tyrosine hydroxylase (pTH, active form rate-limiting enzyme synthesis) effect on dopaminergic context. Pearson correlation analysis revealed positive linear association between pTH immunoreactivity ventral tegmental area midbrain central gray DHEA-implanted, but control-implanted, males. Discussion Taken together, these data suggest starlings modulated effects neurotransmission. More broadly, expand beyond territorial aggression include undirected, affiliative communication.

Language: Английский

---