Comprehensive analysis of cuproptosis-related long noncoding RNA for predicting prognostic and diagnostic value and immune landscape in colorectal adenocarcinoma DOI Creative Commons
Shichao Liu,

Shoucai Zhang,

Yingjie Liu

et al.

Human Genomics, Journal Year: 2023, Volume and Issue: 17(1)

Published: March 13, 2023

Abstract Background Cuproptosis, as a copper-induced mitochondrial cell death, has attracted extensive attention recently, especially in cancer. Although some key regulatory genes have been identified cuproptosis, the related lncRNAs not further studied. Exploring prognostic and diagnostic value of cuproptosis-related (CRLs) colon adenocarcinoma providing guidance for individualized immunotherapy patients are great significance. Results A total 2003 were correlated with cuproptosis considered CRLs. We screened 33 survival-associated CRLs established signature base on 7 training group. The low-risk group had better outcomes both ( P < 0.001) test = 0.016). More exciting, our model showed good prognosis prediction stage I–II 0.020) III–IV 0.001). nomogram could improve accuracy prediction. Interestingly, glucose-related metabolic pathways, which closely to enriched Meanwhile, immune infiltration scores lower high-risk was more sensitive OSI.906 ABT.888, while Sorafenib. Three lncRNAs, FALEC, AC083967.1 AC010997.4, highly expressed serum COAD patients, AUC 0.772, 0.726 0.714, respectively, indicating their valuable value. Conclusions Our research constructed based found three promising markers patients. results provided reference personalized strategies.

Language: Английский

Revealing the role of serum exosomal novel long non-coding RNA NAMPT-AS as a promising diagnostic/prognostic biomarker in colorectal cancer patients DOI

Nehal I. Rizk,

Dina H. Kassem, Ahmed I. Abulsoud

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 352, P. 122850 - 122850

Published: June 18, 2024

Language: Английский

Citations

19
CCDC144NL-AS1/hsa-miR-143-3p/HMGA2 interaction: In-silico and clinically implicated in CRC progression, correlated to tumor stage and size in case-controlled study; step toward ncRNA precision DOI

Yasmine K. Abd El Fattah,

Ahmed I. Abulsoud, Sherihan G. AbdelHamid

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 126739 - 126739

Published: Sept. 9, 2023

Language: Английский

Citations

41
LINC01088 prevents ferroptosis in glioblastoma by enhancing SLC7A11 via HLTF/USP7 axis DOI Creative Commons
Yujie Zhou,

Zhen Zhao,

Jiang Cheng

et al.

Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(3)

Published: Feb. 25, 2025

Glioblastoma multiforme (GBM)is a highly aggressive malignancy of the central nervous system characterized by poor survival rates. Ferroptosis, an iron-dependent cell death pathway, is promising therapeutic target for GBM. However, current treatments targeting pathways have not yielded expected results. Long noncoding RNAs (lncRNAs) been implicated in tumour proliferation, however, their role ferroptosis GBM remains underexplored. This study investigated interplay between lncRNA LINC01088 and to identify novel strategies. We conducted gain- loss-of-function studies assess impact on tumourigenesis both vitro vivo. Bioinformatics, dual-luciferase reporter assays, chromatin immunoprecipitation, RNA pulldown, mass spectrometry, immunoprecipitation (RIP), transcriptome sequencing were utilized elucidate mechanisms underlying expression its downstream effects ferroptosis. The transcription factor specificity protein 1 (SP1) was identified as promoter transcription, which facilitated progression. found inhibit promote malignancy. Mechanistically, stabilized HLTF enhancing interaction with USP7 preventing ubiquitin-mediated degradation. stabilization led upregulation SLC7A11, inhibits Rescue experiments confirmed that altering levels reversed ferroptotic phenotypes associated modulation. revealed SP1/LINC01088/HLTF/USP7/SLC7A11 axis regulates GBM, highlighting potential ferroptosis-dependent treatment. transcriptionally upregulated SP1. acts scaffold platform bind HLTF. USP7, deubiquitinating enzyme HLTF, participates inhibiting ubiquitin-proteasome degradation upregates cells inhibited.

Language: Английский

Citations

1
Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway DOI Creative Commons
Weiwei Zheng,

Yingchang Guo,

Guangtan Zhang

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(6), P. e0287133 - e0287133

Published: June 22, 2023

Long non-coding RNAs (lncRNAs) have been revealed to harbor open reading frames (ORFs) that can be translated into small peptides. The peptides may participate in the pathogenesis of colorectal cancer (CRC). Herein, we investigated role a lncRNA BVES-AS1-encoded peptide tumorigenesis. Through bioinformatic analysis, BVES-AS1 was predicted encoding potential and associated with poor prognosis patients CRC. In CRC cells, validated encode 50-aa-length micro-peptide, named BVES-AS1-201-50aa, through western blotting method. BVES-AS1-201-50aa enhanced cell viability promoted migratory invasive capacities HCT116 SW480 cells vitro , via CCK-8 assay transwell assay, respectively. Immunofluorescence showed increased expression proliferating nuclear antigen (PCNA) matrix metalloproteinase 9 (MMP9) cells. We further verified targeted activated Src/mTOR signaling pathway by co-immunoprecipitation (Co-IP) experiment, qualitative proteomic blotting. Our findings demonstrated could which viability, migration, invasion . current work broadens diversity breadth lncRNAs human carcinogenesis.

Language: Английский

Citations

22
The Interplay between Noncoding RNAs and p21 Signaling in Gastrointestinal Cancer: From Tumorigenesis to Metastasis DOI

Farzad Rahmani,

Mehrdad Zandigohar,

Pegah Safavi

et al.

Current Pharmaceutical Design, Journal Year: 2023, Volume and Issue: 29(10), P. 766 - 776

Published: March 1, 2023

Abstract: Non-coding RNAs (ncRNAs) are emerging as important regulators in various pathological conditions, including human cancers. NcRNAs exert potentially crucial effects on cell cycle progression, proliferation, and invasion cancer cells by targeting cycle-related proteins at transcriptional post-transcriptional levels. As one of the key regulatory proteins, p21 is involved processes, cellular response to DNA damage, growth, invasion, metastasis, apoptosis, senescence. P21 has been shown have either a tumor-suppressive or oncogenic effect depending localization posttranslational modifications. exerts significant both G1/S G2/M checkpoints regulating function cyclin-dependent kinase enzymes (CDKs) interacting with proliferating nuclear antigen (PCNA). an damage separating replication from PCNA inhibiting synthesis resulting G1 phase arrest. Furthermore, negatively regulate checkpoint through inactivation cyclin-CDK complexes. In any caused genotoxic agents, its preservation cyclin B1-CDK1 preventing their activation. Notably, several ncRNAs, lncRNAs miRNAs, be tumor initiation progression regulation signaling axis. this review, we discuss miRNA/lncRNA-dependent mechanisms that gastrointestinal tumorigenesis. A better understanding ncRNAs may help discover novel therapeutic targets cancer.

Language: Английский

Citations

15
Cancer-associated fibroblasts-derived extracellular vesicles carrying lncRNA SNHG3 facilitate colorectal cancer cell proliferation via the miR-34b-5p/HuR/HOXC6 axis DOI Creative Commons
Jiangning Zhao,

Huanrong Lin,

Kunsong Huang

et al.

Cell Death Discovery, Journal Year: 2022, Volume and Issue: 8(1)

Published: Aug. 3, 2022

Cancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) can mediate tumorigenesis. Long noncoding RNA (LncRNA) SNHG3 is implicated in colorectal cancer (CRC) progression. The current study sought to clarify the role of CAFs-EVs carrying CRC cell proliferation. Firstly, CAFs and normal (NFs) were cultured identified, followed by isolation characterization NFs-EVs. cells with or overexpressing SNHG3. effects on proliferation was evaluated using CCK-8, colony formation, EdU staining assays. binding relationships among SNHG3, miR-34b-5p, HuR validated, addition analyzing between HOXC6. Lastly, xenograft tumor model established verify vivo. highly expressed CAFs-EVs, whereas facilitated Mechanically, carried into upregulate expression competitively promote HOXC6, enhance HOXC6 transcription. miR-34b-5p over-expression silencing annulled effect CAFs-EVs. via miR-34b-5p/HuR/HOXC6 axis Collectively, our findings indicated that sponging finally transcription, thereby facilitating

Language: Английский

Citations

21
LncRNA OTUD6B-AS1 overexpression promoted GPX4-mediated ferroptosis to suppress radioresistance in colorectal cancer DOI

Zilang Zhang,

Baolong Ye,

Yiban Lin

et al.

Clinical & Translational Oncology, Journal Year: 2023, Volume and Issue: 25(11), P. 3217 - 3229

Published: May 15, 2023

Language: Английский

Citations

12
LINC01977 promotes colorectal cancer growth and metastasis by enhancing aerobic glycolysis via the ERK/c-Myc axis DOI Open Access
Jie Wu, Qiwen Chen, Yunliang Wang

et al.

Journal of Gastrointestinal Oncology, Journal Year: 2024, Volume and Issue: 15(1), P. 271 - 285

Published: Feb. 1, 2024

Background: How colorectal cancer (CRC) gain the ability to growth and metastasis remains largely unknown. Findings from preceding studies have revealed participation of long non-coding RNAs (lncRNAs) in CRC progression. However, role LINC01977 unexplored. This study aims explore function underlying mechanism CRC. Methods: The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) datasets were used analyze expression its correlation with prognosis. In our research, we explored influence on progression such as cell proliferation, migration, invasion, aerobic glycolysis, identified fundamental molecular using vitro lines vivo mouse xenograft models. Results: exhibited significantly elevated tissues lines, level was correlated malignant clinicopathological characteristics negative Furthermore, both tests LINC01977's facilitating proliferation metastasis. significant part glycolysis also discovered. With an aim uncover mechanism, investigated effect c-Myc, a key gene glycolysis. results showed that regulated c-Myc stability via extracellular signal-regulated kinase (ERK)-mediated phosphorylation, LINC01977-mediated activated vital glycolysis-related genes HK2, PGK1, LDHA, GLUT1. Rescue experiments further confirmed promoted metastasis, c-Myc. Conclusions: is first report facilitates through suggesting potential therapeutic target for treatment.

Language: Английский

Citations

4
Unboxing the network among long non-coding RNAs and TGF-β signaling in cancer DOI Creative Commons
Dorival Mendes Rodrigues‐Junior, Aristidis Moustakas

Upsala Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 129, P. e10614 - e10614

Published: March 27, 2024

Deeper analysis of molecular mechanisms arising in tumor cells is an unmet need to provide new diagnostic and therapeutic strategies prevent treat tumors. The transforming growth factor β (TGF-β) signaling has been steadily featured biology linked poor prognosis cancer patients. One pro-tumorigenic mechanism induced by TGF-β the epithelial-to-mesenchymal transition (EMT), which can initiate dissemination, enrich stem cell population, increase chemoresistance. signals via SMAD proteins, ubiquitin ligases, protein kinases modulates expression protein-coding non-coding RNA genes, including those encoding larger than 500 nt transcripts, defined as long RNAs (lncRNAs). Several reports have shown lncRNAs regulating malignant phenotypes directly affecting epigenetic processes, transcription, post-transcriptional regulation. Thus, this review aims update summarize impact on function such regulators signaling, how these networks might specific hallmarks cancer.

Language: Английский

Citations

4
Identification of MYC and STAT3 for early diagnosis based on the long noncoding RNA-mRNA network and bioinformatics in colorectal cancer DOI Creative Commons

Kunhou Yao,

Hao Fan,

Tiancheng Yang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 3, 2025

Colorectal cancer (CRC) ranks among the top three cancers globally in both incidence and mortality, posing a significant public health challenge. Most CRC cases are diagnosed at intermediate to advanced stages, reliable biomarkers for early detection lacking. Long non-coding RNAs (lncRNAs) have been implicated various cancers, including CRC, playing key roles tumor development, progression, prognosis. A comprehensive search of PubMed database was conducted identify relevant studies on diagnosis CRC. Bioinformatics analysis performed explore lncRNA-mRNA networks, leading identification five potential blood biomarkers. Expression carried out using GEPIA GEO online databases, focusing MYC STAT3. Differential expression between normal tissues assessed, followed by Receiver Operating Characteristic (ROC) evaluate diagnostic these markers. Quantitative Real-Time PCR (qRT-PCR) validate STAT3 levels, findings were further confirmed Human Protein Atlas (HPA) database. Database revealed differential tissues. ROC demonstrated qRT-PCR validation patterns observed databases. Validation through HPA supported findings, confirming as Our results suggest that promising offering new insights into its pathophysiology targeted therapies.

Language: Английский

Citations

0