The combination of serum lncRNA PTTG3P and mRNA PTTG1 serves as a diagnostic and prognostic marker for hepatocellular carcinoma DOI Creative Commons
Shunwang Cao, Fei Zhong, Xueying Chen

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(2)

Published: Nov. 29, 2024

Long noncoding RNA (lncRNA) PTTG3P has been demonstrated to participate in the development of hepatocellular carcinoma (HCC) by targeting mRNA PTTG1. The present study aimed investigate diagnostic efficacy serum lncRNA PTTG3P, PTTG1 and their combination for diagnosis prognosis HCC. A total 373 participants were enrolled study, including 73 patients with HCC, 100 chronic hepatitis B (CHB), liver cirrhosis (LC) healthy controls (HCs). expression levels RNAs quantified reverse transcription‑quantitative PCR. association between clinical characteristics was further analyzed. Receiver operating characteristic (ROC) curve area under (AUC) analyses performed estimate ability combinations other biomarkers results revealed that markedly increased HCC CHB compared HCs. Additionally, postoperative significantly lower than preoperative concentrations 36 paired Spearman's correlation coefficient analysis showed correlated aspartate transaminase (AST). ROC both had a significant predictive value AUC values alone 0.636 0.634, respectively. Furthermore, combining PTTG1, α‑fetoprotein (AFP), alanine aminotransferase (ALT), AST, γ‑glutamyl transpeptidase (GGT) alkaline phosphatase (ALP) value. best performance AFP, ALT, GGT ALP an 0.959, sensitivity 90.4% specificity 98.0%. In conclusion, AFP appeared be noninvasive biomarker comparatively high

Language: Английский

Non-coding RNA mediated regulation of PI3K/Akt pathway in hepatocellular carcinoma: Therapeutic perspectives DOI
Md Sadique Hussain, Ehssan Moglad, Muhammad Afzal

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 258, P. 155303 - 155303

Published: April 28, 2024

Language: Английский

Citations

18
LncRNA LINC01128 promotes prostate cancer cell proliferation, metastasis, and epithelial-mesenchymal transition by modulating miR-27b-3p DOI Creative Commons
Yuhui Zhao, Zhigang Zhang, Yi Zheng

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2025, Volume and Issue: 151(3)

Published: March 4, 2025

Language: Английский

Citations

1
Long non-coding RNA AC133552.2: biomarker and therapeutic target in osteosarcoma via PANoptosis gene screening DOI Creative Commons

Wenrui Zhao,

Yameng Wang, Junchao Zhu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 17, 2025

Osteosarcoma, the most common primary bone cancer in children and adolescents, presents significant challenges, particularly metastasis recurrence, resulting poor survival rates. This study explores role of PANoptosis—a complex cell death mechanism involving pyroptosis, apoptosis, necroptosis—in osteosarcoma by identifying relevant long non-coding RNAs (lncRNAs) their prognostic significance. Bioinformatics analyses used RNA expression data from GEO TARGET databases to identify differentially expressed genes (DEGs) PANoptosis-related (PRGs). Co-expression analysis revealed lncRNAs linked PRGs, forming a risk model. Five PRGs two were significantly associated with prognosis, model showing high predictive accuracy (AUC 0.876, 0.787, 0.794 for 1, 3, 5 years). Notably, lncRNA AC133552.2 was downregulated tissues, correlating reduced immune infiltration. Silencing promoted proliferation migration, while overexpression inhibited tumor growth metastasis, confirmed xenograft models. emerges as potential biomarker therapeutic target, future research needed explore its molecular mechanisms clinical application.

Language: Английский

Citations

0
Dynamic role of long noncoding RNA in liver diseases: pathogenesis and diagnostic aspects DOI Creative Commons
Ningning Chen, Yunxia Li,

Xiaoying Li

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 14, 2025

Liver disease (LD) is complex pathological condition that has emerged as a major threat to human health and the quality of life. Nonetheless, molecular mechanisms underlying pathogenesis LD have not yet been fully elucidated. Recently, large amount evidence shown long noncoding RNAs (lncRNAs) play important roles in diverse biological processes liver. The dysregulation lncRNAs liver, for example, can affect tumor proliferation, migration, invasion, contribute hepatic metabolism disorder lipid glucose, shape tumoral microenvironment. Thus, comprehensive understanding functional may provide new perspectives development novel diagnostic therapeutic tools. In present review, we summarize current findings on relationship between LD, including modes action lncRNAs, significance especially hepatocellular carcinoma (HCC) well some non-neoplastic disorders, potential use biomarkers targets LD.

Language: Английский

Citations

0
Delving into the Role of lncRNAs in Papillary Thyroid Cancer: Upregulation of LINC00887 Promotes Cell Proliferation, Growth and Invasion DOI Open Access
Cristina Tous,

Carmen Muñoz-Redondo,

Ángela Gavilán

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1587 - 1587

Published: Jan. 27, 2024

Papillary thyroid carcinoma (PTC) is the most common histological category of cancer. In recent years, there has been an increasing number studies on lncRNAs in PTC. Long intergenic non-protein coding RNA 887 (LINC00887) a critical oncogene developing other cancers. LINC00887 upregulated PTC samples but its role currently unclear. This study aimed to investigate impact disruption expression progression. We performed CRISPR/Cas9 strategy for truncation BCPAP and TPC1 cell lines. Functional assays showed that knockdown both cells reduced proliferation, colony formation migration, delayed cycle, increased apoptosis. These results strengthened cancer first time this lncRNA could be potential PTC, acting as tumor promoter. Modulation immune system may one etiopathogenic mechanisms shown by observed influence PD-L1 expression. addition, biological pathways identified date, such EMT, Wnt/β-catenin signaling pathway or FRMD6-Hippo also relevant regulatory operating

Language: Английский

Citations

3
Long noncoding RNA DDX11-AS1 represses sorafenib-induced ferroptosis in hepatocellular carcinoma cells via Nrf2-Keap1 pathway DOI Creative Commons
Liang Wang, Liming Wang

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 10, 2024

Sorafenib, a first-line therapeutic option for advanced hepatocellular carcinoma (HCC), faces formidable challenge in the form of emerging resistance. Recently, oncogene DDX11 antisense RNA 1 (DDX11-AS1) has been implicated various cancers, including HCC. However, its role sorafenib resistance remains unknown. Our findings reveal that DDX11-AS1 is upregulated sorafenib-resistant HCC cells, contributing to their by suppressing ferroptosis. Further investigation elucidated mechanism which activates antioxidant Nrf2-Keap1 pathway. By interacting with Nrf2 and hindering association Keap1, enhances stability nuclear translocation Nrf2. In summary, our study unveils potent as an enhancer resistance, inhibiting sorafenib-induced ferroptosis through activation pathway These offer promising strategy overcome effectively treat

Language: Английский

Citations

3
RNA splicing variants of the novel long non-coding RNA, CyKILR, possess divergent biological functions in non-small cell lung cancer. DOI Creative Commons
Xiujie Xie, H. Patrick MacKnight,

Amy L. Lu

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 36(1), P. 102412 - 102412

Published: Dec. 5, 2024

Language: Английский

Citations

2
Transcriptome-Wide Dynamics of m7G-Related LncRNAs during the Progression from HBV Infection to Hepatocellular Carcinoma DOI Creative Commons

Min Shi,

Shunshun Zhu,

Linying Sun

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2023, Volume and Issue: 28(12)

Published: Dec. 19, 2023

Background: The functional ramifications of internal N7-methylguanosine (m7G) modification on RNAs have recently come to light, yet its regulatory influence long noncoding (lncRNAs) during the inflammatory-carcinogenesis transformation process in hepatitis B virus (HBV)-mediated hepatocellular carcinoma (HCC) remains largely unexplored. Methods: Clinical surgical samples encompassing HBV-related HCC, comprising both HCC tissue (tumor group, HBV+) and corresponding adjacent liver (paracancerous HBV+), were collected for analysis. Additional normal tissues (normal HBV-) acquired from patients with hepatic hemangioma, serving as controls. Employing MeRIP-seq, differential m7G levels lncRNAs across these groups compared identify a subset exhibiting continuous dynamic changes modification. Subsequently, vitro validation was conducted. Results: A total 856 exhibited alterations when paracancerous tissue. Similarly, 1775 displayed comparing For intergroup comparison, orthogonal analysis revealed that 6 consistently demonstrated hyper-m7G In confirmed among lncRNAs, TEKT4P2 DNM1P41 modification-dependent expression. Conclusions: This study provides comprehensive lncRNA HBV-mediated HCC. findings highlight potential multiple undergo changes, identified promising molecular targets within this intricate landscape.

Language: Английский

Citations

4
Comprehensive analysis identifies long non-coding RNA RNASEH1-AS1 as a potential prognostic biomarker and oncogenic target in hepatocellular carcinoma DOI Open Access
Jin Sun

American Journal of Cancer Research, Journal Year: 2024, Volume and Issue: 14(3), P. 996 - 1014

Published: Jan. 1, 2024

RNASEH1-AS1, a long non-coding RNA (lncRNA) divergently transcribed from the antisense strand of its neighboring protein-coding gene ribonuclease H1 (RNASEH1), has recently been demonstrated to be involved in tumor progression. However, association between RNASEH1-AS1 and hepatocellular carcinoma (HCC) remains unclear. In present study, first, expression HCC correlation with clinicopathological features, prognosis, diagnosis, immune cell infiltration patients was inspected using relevant R packages based on The Cancer Genome Atlas (TCGA) data. found up-regulated most cancer types, including HCC, overexpression significantly associated histologic grade AFP level as well poor an independent risk factor affecting overall survival good diagnostic prognostic values for HCC. inversely plasmacytoid dendritic cells (pDC), B neutrophils. Second, total 1109 positively co-expressed genes (PCEGs) were screened out by analysis batches (|Spearman's r| >0.4 adjusted

Language: Английский

Citations

1
RNA splicing variants of the novel long non-coding RNA, CyKILR, possess divergent biological functions in non-small cell lung cancer DOI
Xiujie Xie, H. Patrick MacKnight,

Amy L. Lu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 8, 2024

ABSTRACT The CDKN2A gene, responsible for encoding the tumor suppressors p16(INK4A) and p14(ARF), is frequently inactivated in non-small cell lung cancer (NSCLC). Herein, an uncharacterized long non-coding RNA (lncRNA) (ENSG00000267053) on chromosome 19p13.12 was found to be overexpressed NSCLC cells with active, wild-type gene. This lncRNA, named Cy clin-Dependent K inase I nhibitor 2A-regulated l nc R NA (CyKILR), also correlated active WT STK11 which encodes suppressor, Liver kinase B1. CyKILR displayed two splice variants, CyKILRa (exon 3 included) CyKILRb excluded), are cooperatively regulated by as knockdown of both suppressor genes required induce a significant loss exon inclusion mature RNA. localized nucleus, its downregulation using antisense oligonucleotides enhanced cellular proliferation, migration, clonogenic survival, incidence. In contrast, cytoplasm, siRNA reduced Transcriptomics analyses revealed enhancement apoptotic pathways concomitant suppression key cycle demonstrating tumor-suppressive role. inhibited microRNAs indicating oncogenic nature. These findings elucidate intricate roles lncRNAs signaling tumorigenesis.

Language: Английский

Citations

1