The Exercise and Heart Transplant (Enea) Trial – a Registry-Based Randomized Controlled Trial Evaluating the Safety and Efficacy of Cardiac Telerehabilitation after Heart Transplant DOI
Paolo Pedersini, Silvia Picciolini, Francesca Di Salvo

et al.

Published: Jan. 1, 2023

Background: Heart transplant (HTx) is gold-standard therapy for patients with end-stage heart failure. Cardiac rehabilitation (CR) a multidisciplinary intervention shown to improve cardiovascular prognosis and quality of life. The aim in this randomized controlled trial explore the safety efficacy cardiac telerehabilitation after HTx. In addition, biomarkers outcomes will be identified, as data that enable treatment tailored patient phenotype.Methods: Patients HTx recruited at IRCCS S. Maria Nascente – Fondazione Don Gnocchi, Milan, Italy (n=40). Consenting participants randomly allocated either two groups (1:1): an group who receive on-site CR followed by 12 weeks telerehabilitation, or control standard homecare exercise programme. Recruitment began on 20th May 2023 expected continue until 2025. Socio-demographic characteristics, lifestyle, health status, events, cognitive function, anxiety depression symptoms, life assessed, well capacity muscular endurance. Participants evaluated before intervention, post-CR 6 months. analysis circulating extracellular vesicles using Surface Plasmon Resonance imaging (SPRi), based rehabilomic approach, applied both pre- post-CR.Conclusion: This study approach used investigate biomolecular phenotypization patients.

Language: Английский

Proteomics provides insights into the theranostic potential of extracellular vesicles DOI
Morteza Abyadeh, Mehdi Alikhani, Mehdi Mirzaei

et al.

Advances in protein chemistry and structural biology, Journal Year: 2023, Volume and Issue: unknown, P. 101 - 133

Published: Dec. 1, 2023

Language: Английский

Citations

7
Biological functions and biomedical applications of extracellular vesicles derived from blood cells DOI
Kai‐Feng Pan, Yiwei Zhu, Pengyu Chen

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 222, P. 43 - 61

Published: June 6, 2024

Language: Английский

Citations

2
Applications of plant‐derived extracellular vesicles in medicine DOI Creative Commons

Yawen Zhu,

Junqi Zhao, Haoran Ding

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Plant-derived extracellular vesicles (EVs) are promising therapeutic agents owing to their natural abundance, accessibility, and unique biological properties. This review provides a comprehensive exploration of the potential plant-derived EVs emphasizes anti-inflammatory, antimicrobial, tumor-inhibitory effects. Here, we discussed advancements in isolation purification techniques, such as ultracentrifugation size-exclusion chromatography, which critical for maintaining functional integrity these nanovesicles. Next, investigated diverse administration routes carefully weighed respective advantages challenges related bioavailability patient compliance. Moreover, elucidated multifaceted mechanisms action EVs, including roles anti-inflammation, antioxidation, antitumor activity, modulation gut microbiota. We also impact on specific diseases cancer inflammatory bowel disease, highlighting importance addressing current production scalability, regulatory compliance, immunogenicity. Finally, proposed future research directions optimizing EV extraction developing targeted delivery systems. Through efforts, envision seamless integration into mainstream medicine, offering safe potent alternatives across various medical disciplines.

Language: Английский

Citations

2
Exploring the potential of plasma and adipose mesenchymal stem cell-derived extracellular vesicles as novel platforms for neuroinflammation therapy DOI Creative Commons
Rummenigge Oliveira Silva, Mohamed Haddad, Hermine Counil

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 377, P. 880 - 898

Published: Dec. 7, 2024

Persistent reactive oxygen species (ROS) and neuroinflammation contribute to the onset progression of neurodegenerative diseases, underscoring need for targeted therapeutic strategies mitigate these effects. Extracellular vesicles (EVs) show promise in drug delivery due their biocompatibility, ability cross biological barriers, specific interactions with cell tissue receptors. In this study, we demonstrated that human plasma-derived EVs (pEVs) exhibit higher brain-targeting specificity, while adipose-derived mesenchymal stem cells (ADMSC-EVs) offer regenerative immunomodulatory properties. We further investigated potential as carriers brain-targeted delivery, using Donepezil (DNZ) model drug. DNZ, a cholinesterase inhibitor commonly used Alzheimer's disease (AD), also has neuroprotective anti-inflammatory The size ranged from 50 300 nm surface charge below -30 mV. Both formulations showed rapid cellular internalization, without toxicity, blood-brain barrier (BBB) zebrafish model. have analyzed antioxidant actions pEVs-DNZ ADMSC-EVs-DNZ presence lipopolysaccharide (LPS). ADMSC-EVs significantly reduced inflammatory mediators released by HMC3 microglial treatment lowered both phagocytic activity ROS levels cells. vivo experiments larvae revealed EV proliferation exhibited Overall, study highlights loaded DNZ novel approach treating underlying various diseases.

Language: Английский

Citations

2
Distinct roles of ascorbic acid in extracellular vesicles and free form: Implications for metabolism and oxidative stress in presymptomatic Huntington's Disease DOI Creative Commons

Felipe Beltrán,

Leandro Torres-Díaz L,

Paulina Troncoso-Escudero

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 227, P. 521 - 535

Published: Dec. 9, 2024

Huntington's disease (HD) is a neurodegenerative disorder caused by CAG trinucleotide repeat expansion in the first exon of huntingtin gene. The protein (Htt) ubiquitously expressed and localized several organelles, including endosomes, where it plays an essential role intracellular trafficking. Presymptomatic HD associated with failure energy metabolism oxidative stress. Ascorbic acid potent antioxidant that key modulating neuronal highly concentrated brain. During synaptic activity, neurons take up ascorbic released glial cells; however, this process disrupted HD. In study, we aim to elucidate molecular cellular mechanisms underlying dysfunction. Using electrophysiological approach presymptomatic YAC128 slices, observed decreased flux from astrocytes neurons, which altered metabolic substrate preferences. efflux recycling were also cultured mice. We confirmed our findings using GFAP-HD160Q, mice model expressing mutant N-terminal Htt mainly astrocytes. For time, demonstrated via extracellular vesicles (EVs). Decreased number particles exosomal markers EV fractions Htt-KD cells. reduced multivesicular bodies (MVBs) striatum electron microscopy, suggesting alters MVB biogenesis. EVs containing effectively reactive oxygen species, whereas "free" played These suggest early redox imbalance arises release acid-containing Meanwhile, decrease likely contributes impairment.

Language: Английский

Citations

2
Converging protective pathways: Exploring the linkage between physical exercise, extracellular vesicles and oxidative stress DOI
Veronica Lisi, Giorgia Senesi, Carolina Balbi

et al.

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 208, P. 718 - 727

Published: Sept. 20, 2023

Language: Английский

Citations

5
Extracellular vesicles from II trimester human amniotic fluid as paracrine conveyors counteracting oxidative stress DOI Creative Commons
Giorgia Senesi, Laura Guerricchio,

Maddalena Ghelardoni

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 75, P. 103241 - 103241

Published: June 17, 2024

We previously demonstrated that the human amniotic fluid (hAF) from II trimester of gestation is a feasible source stromal progenitors (human stem cells, hAFSC), with significant paracrine potential for regenerative medicine. Extracellular vesicles (EVs) separated and concentrated hAFSC secretome can deliver pro-survival, proliferative, anti-fibrotic cardioprotective effects in preclinical models skeletal cardiac muscle injury. While hAFSC-EVs isolation be significantly influenced by vitro cell culture, here we profiled EVs directly hAF as an alternative option investigated their against oxidative stress. samples were obtained leftover material prenatal diagnostic amniocentesis following written informed consent. size exclusion chromatography ultracentrifugation. hAF-EVs assessed nanoparticle tracking analysis, transmission electron microscopy, Western Blot, flow cytometry; metabolic activity was evaluated oximetric luminometric analyses cargo proteomics RNA sequencing. hAF-EV tested stress dysfunction on murine C2C12 cells 3D microtissue. Our protocol resulted yield 6.31 ± 0.98 × 109 particles per milliliter showing round cup-shaped morphology 209.63 6.10 nm average size, relevant expression CD81, CD63 CD9 tetraspanin markers. enriched CD133/1, CD326, CD24, CD29, SSEA4 able to produce ATP oxygen consumption. reduced survival, priming rescue viability, notable recovery synthesis concomitant reduction damage lipid peroxidation activity. microtissues treated experiencing H2O2 TGFβ stimulation showed improved survival remarkable decrease onset fibrosis. results suggest represent counteract target thus offering novel candidate therapeutic

Language: Английский

Citations

1
Exploring the Impact of Exercise-Derived Extracellular Vesicles in Cancer Biology DOI Creative Commons

Monica Silvestri,

Elisa Grazioli, Guglielmo Duranti

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 701 - 701

Published: Sept. 6, 2024

Cancer remains a major challenge in medicine, prompting exploration of innovative therapies. Recent studies suggest that exercise-derived extracellular vesicles (EVs) may offer potential anti-cancer benefits. These small, membrane-bound particles, including exosomes, carry bioactive molecules such as proteins and RNA mediate intercellular communication. Exercise has been shown to increase EV secretion, influencing physiological processes like tissue repair, inflammation, metabolism. Notably, preclinical have demonstrated EVs can inhibit tumor growth, reduce metastasis, enhance treatment response. For instance, study using animal models, were suppress proliferation breast colon cancers. Another reported these reduced metastatic by decreasing the migration invasion cancer cells. Additionally, exercise-induced found effectiveness chemotherapy sensitizing cells treatment. This review highlights emerging role circulating biomolecules, particularly EVs, biology. It discusses mechanisms through which impact progression, challenges translating findings clinical practice, future research directions. Although this area is still limited, current could play crucial spreading promote better health patients. Understanding profiles lead therapies, exercise mimetics or targeted drugs, treat cancer.

Language: Английский

Citations

1
Harnessing the therapeutic potential of exercise in extracellular vesicle-based therapy in metabolic disease associated cardiovascular complications DOI Creative Commons

Nimna Perera,

Miles J. De Blasio, Mark A. Febbraio

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 226, P. 230 - 236

Published: Nov. 15, 2024

Cardiovascular disease (CVD) is a leading cause of mortality, affecting ∼18 million individuals each year. Obesity and type 2 diabetes mellitus in particular, both chronic metabolic disorders, are risk factors for CVD. The salutary effects physical activity preventing ameliorating CVD have long been acknowledged, as it improves glucose lipid homeostasis, alongside attenuating oxidative damage, increasing mitochondrial function, ultimately improving cardiac function. Exercise serves catalyst the secretion extracellular vesicles (EVs), facilitating inter-tissue communication, by which tissues can deliver important signals from one tissue to another. In recent years, an number studies focused on cargo encapsulated within exercise-derived EVs, well orchestration crosstalk aimed at modulating metabolism function CVDs. precise mechanisms underpinning cardioprotective properties however, remains only partially elucidated. This review explores novel EV based therapeutic options and, EVs derived models exercise alter enhance cardiovascular outcomes.

Language: Английский

Citations

1
The Exercise aNd hEArt transplant (ENEA) trial – a registry-based randomized controlled trial evaluating the safety and efficacy of cardiac telerehabilitation after heart transplant DOI Creative Commons
Paolo Pedersini, Silvia Picciolini, Francesca Di Salvo

et al.

Contemporary Clinical Trials, Journal Year: 2023, Volume and Issue: 136, P. 107415 - 107415

Published: Dec. 17, 2023

Heart transplant (HTx) is gold-standard therapy for patients with end-stage heart failure. Cardiac rehabilitation (CR) a multidisciplinary intervention shown to improve cardiovascular prognosis and quality of life. The aim in this randomized controlled trial explore the safety efficacy cardiac telerehabilitation after HTx. In addition, biomarkers outcomes will be identified, as data that enable treatment tailored patient phenotype. Patients HTx recruited at IRCCS S. Maria Nascente – Fondazione Don Gnocchi, Milan, Italy (n = 40). Consenting participants randomly allocated either two groups (1:1): an group who receive on-site CR followed by 12 weeks telerehabilitation, or control standard homecare exercise programme. Recruitment began on 20th May 2023 expected continue until 2025. Socio-demographic characteristics, lifestyle, health status, events, cognitive function, anxiety depression symptoms, life assessed, well capacity muscular endurance. Participants evaluated before intervention, post-CR 6 months. analysis circulating extracellular vesicles using Surface Plasmon Resonance imaging (SPRi), based rehabilomic approach, applied both pre- post-CR. This study approach used investigate biomolecular phenotypization patients. Trial registration number: ClinicalTrials.gov Identifier: NCT05824364.

Language: Английский

Citations

2