Environmental Science and Pollution Research,
Journal Year:
2023,
Volume and Issue:
30(32), P. 79315 - 79334
Published: June 7, 2023
Abstract
Wastewater-based
epidemiology
has
been
widely
used
as
a
cost-effective
method
for
tracking
the
COVID-19
pandemic
at
community
level.
Here
we
describe
COVIDBENS,
wastewater
surveillance
program
running
from
June
2020
to
March
2022
in
treatment
plant
of
Bens
A
Coruña
(Spain).
The
main
goal
this
work
was
provide
an
effective
early
warning
tool
based
help
decision-making
both
social
and
public
health
levels.
RT-qPCR
procedures
Illumina
sequencing
were
weekly
monitor
viral
load
detect
SARS-CoV-2
mutations
wastewater,
respectively.
In
addition,
own
statistical
models
applied
estimate
real
number
infected
people
frequency
each
emerging
variant
circulating
community,
which
considerable
improved
strategy.
Our
analysis
detected
6
waves
with
concentrations
between
10
3
RNA
copies/L.
system
able
anticipate
outbreaks
during
8–36
days
advance
respect
clinical
reports
and,
emergence
new
variants
such
Alpha
(B.1.1.7),
Delta
(B.1.617.2),
Omicron
(B.1.1.529
BA.2)
42,
30,
27
days,
respectively,
before
did.
Data
generated
here
helped
local
authorities
managers
give
faster
more
efficient
response
situation,
also
allowed
important
industrial
companies
adapt
their
production
situation.
wastewater-based
developed
our
metropolitan
area
(Spain)
served
powerful
combining
monitoring
over
time.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 20, 2025
Wastewater-based
epidemiology
(WBE)
has
been
employed
for
monitoring
the
presence
of
SARS-CoV-2
infected
population.
Herein,
study
aims
to
apply
WBE
surveillance
and
in
Bangkok,
where
highest
official
covid-19
cases
reported
Thailand,
during
fully
opening
international
tourists
early
2023.
A
total
200
wastewater
samples
(100
influent
100
effluent
samples)
were
collected
from
10
treatment
plants
(WWTPs)
January–May
RNA
was
detected
by
real
time
qRT-PCR
with
accounting
51%
(102/200).
Of
these,
88%
(88/100)
14%
(14/100)
samples,
respectively.
The
concentration
ranged
4.76
×
102–1.48
105
copies/L.
amount
increased
approximately
4
times
lag
phase
(January–March)
log
(April–May).
Spearman's
correlation
coefficient
revealed
that
between
estimated
population
weekly
statistically
significant
(p-value
=
0.017).
had
a
relationship
(r
0.481,
p-value
<
0.001).
Lag
analysis
warning
1–3
weeks
before
rising
observed.
GIS
applied
spatial-temporal
at
province
level,
suggesting
dashboard
should
be
further
developed.
ChemistrySelect,
Journal Year:
2025,
Volume and Issue:
10(13)
Published: April 1, 2025
Abstract
The
COVID‐19
pandemic
outbreak
raised
major
concerns
in
public
health
globally,
raising
the
crucial
need
for
development
of
methods
to
monitor
spread
communities
worldwide.
Wastewater‐based
epidemiology
(WBE)
surveillance
has
been
used
as
a
novel
tool
outbreaks
because
its
affordability
and
efficiency
tracking
infectious
contaminants.
Unlike
other
means
tracking,
wastewater
is
independent
individuals
having
accessibility
healthcare,
doctor
visits,
or
infection
testing
availability.
Consequently,
considerable
awareness
complete
infections,
including
at
community
level.
In
WBE
studies,
polymerase
chain
reaction‐based
(PCR)
techniques
are
referred
“gold‐standard”
method
detecting
SARS‐CoV‐2
many
countries.
Nevertheless,
despite
extensive
sensitive
selective
PCR‐based
methods,
these
have
shown
some
limitations
that
hinder
their
application,
such
requirement
repeated
heating
cooling
cycles
analysis
time
3–4
h.
alternative
do
not
rely
on
same
consumables
conventionally
employed
electrochemical
biosensing,
environmental
water
samples
offers
favorable
advantages
improved
turnaround
times
portability.
However,
currently
highly
focused
clinical
applications
than
wastewater.
This
review
focuses
disadvantages
associated
with
conventional
alternative:
electrochemical‐bioreceptor‐based
technique
SARS‐CoV‐2.
addition,
highlights
broad
use
WBE,
binding
affinity
various
bioreceptors
toward
viral
proteins,
enhancing
analytical
properties
biosensors
integration
techniques.
integrated
systems,
especially,
electrochemical‐CRISPR
based,
high
sensitivities
(down
concentrations
atto‐molar),
potential
application
low‐resource
areas.
Environmental Research,
Journal Year:
2023,
Volume and Issue:
231, P. 115911 - 115911
Published: April 25, 2023
Tuberculosis
(TB)
remains
a
persistent
challenge
to
public
health
and
presents
substantial
menace,
especially
in
developing
nations
of
sub-Saharan
Africa.
It
exerts
considerable
strain
on
healthcare
systems
these
regions.
Effective
control
requires
reliable
surveillance,
which
can
be
improved
by
incorporating
environmental
data
alongside
clinical
data.
Molecular
advances
have
led
the
development
alternative
surveillance
methods,
such
as
wastewater-based
epidemiology.
This
studyinvestigated
presence,
concentration,
diversity
Mycobacterium
tuberculosis
complex,
cause
TB,
from
six
African
countries:
Ghana,
Nigeria,
Kenya,
Uganda,
Cameroon,
South
Samples
were
collected
wastewater
treatment
plants.
All
samples
found
contain
species
that
been
linked
TB
both
humans
animals,
including
tuberculosis,
bovis,
africanum,
caprae,
at
varying
concentrations.
The
highest
median
concentration
was
reaching
up
4.7
Log
copies/ml
for
MTBC,
4.6
M.
3.4
africanum.
presence
africanum
outside
West
Africa
Africa,
Uganda
could
indicate
spread
pathogen.
study
underscores
usefulness
epidemiology
tracking
shows
even
treated
may
pathogens,
posing
potential
risks.
Environmental Science and Pollution Research,
Journal Year:
2023,
Volume and Issue:
30(32), P. 79315 - 79334
Published: June 7, 2023
Abstract
Wastewater-based
epidemiology
has
been
widely
used
as
a
cost-effective
method
for
tracking
the
COVID-19
pandemic
at
community
level.
Here
we
describe
COVIDBENS,
wastewater
surveillance
program
running
from
June
2020
to
March
2022
in
treatment
plant
of
Bens
A
Coruña
(Spain).
The
main
goal
this
work
was
provide
an
effective
early
warning
tool
based
help
decision-making
both
social
and
public
health
levels.
RT-qPCR
procedures
Illumina
sequencing
were
weekly
monitor
viral
load
detect
SARS-CoV-2
mutations
wastewater,
respectively.
In
addition,
own
statistical
models
applied
estimate
real
number
infected
people
frequency
each
emerging
variant
circulating
community,
which
considerable
improved
strategy.
Our
analysis
detected
6
waves
with
concentrations
between
10
3
RNA
copies/L.
system
able
anticipate
outbreaks
during
8–36
days
advance
respect
clinical
reports
and,
emergence
new
variants
such
Alpha
(B.1.1.7),
Delta
(B.1.617.2),
Omicron
(B.1.1.529
BA.2)
42,
30,
27
days,
respectively,
before
did.
Data
generated
here
helped
local
authorities
managers
give
faster
more
efficient
response
situation,
also
allowed
important
industrial
companies
adapt
their
production
situation.
wastewater-based
developed
our
metropolitan
area
(Spain)
served
powerful
combining
monitoring
over
time.
