Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(7), P. 1430 - 1430
Published: July 14, 2024
Wastewater
monitoring
provides
essential
information
about
water
quality
and
the
degree
of
contamination.
Monitoring
these
waters
helps
identify
manage
risks
to
public
health,
prevent
spread
disease,
protect
environment.
Standardizing
appropriate
most
accurate
methods
for
isolation
identification
viruses
in
wastewater
is
necessary.
This
review
aims
present
major
classes
wastewater,
as
well
concentration,
isolation,
assess
health
implement
corrective
measures
control
viral
infections.
Last
but
not
least,
we
propose
evaluate
current
strategies
treatment
new
alternative
disinfection.
The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
838, P. 155828 - 155828
Published: May 16, 2022
SARS-CoV-2
RNA
quantification
in
wastewater
is
an
important
tool
for
monitoring
the
prevalence
of
COVID-19
disease
on
a
community
scale
which
complements
case-based
surveillance
systems.
As
novel
variants
concern
(VOCs)
emerge
there
also
need
to
identify
primary
circulating
community,
accomplished
date
by
sequencing
clinical
samples.
Quantifying
offers
cost-effective
means
augment
these
efforts.
In
this
study,
N1
concentrations
and
daily
loadings
were
determined
compared
data
collected
as
part
national
programme
determine
validity
monitor
infection
spread
greater
Dublin
area.
Further,
samples
was
conducted
lineages
Dublin.
Finally,
digital
PCR
employed
whether
VOCs,
Alpha
Delta,
quantifiable
from
wastewater.
No
lead
or
lag
time
observed
between
trends
significantly
correlated
with
notification
confirmed
cases
through
preceding
collection
5-day
average.
This
demonstrates
that
viral
Dublin's
mirrors
community.
Clinical
sequence
demonstrated
increased
during
Ireland's
third
wave
coincided
introduction
variant,
while
fourth
Delta
variant.
Interestingly,
variant
detected
prior
first
genome
being
sequenced
samples,
identified
at
same
work
infections
highlights
its
effectiveness
identifying
may
prove
useful
when
capacity
limited.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Oct. 10, 2023
Abstract
As
global
SARS-CoV-2
burden
and
testing
frequency
have
decreased,
wastewater
surveillance
has
emerged
as
a
key
tool
to
support
clinical
efforts.
The
aims
of
this
study
were
identify
characterize
variants
in
samples
collected
from
urban
centers
across
South
Africa.
Here
we
show
that
sequencing
analyses
are
temporally
concordant
with
genomic
reveal
the
presence
multiple
lineages
not
detected
by
surveillance.
We
genomics
can
epidemiological
investigations
reliably
recovering
prevalence
local
circulating
variants,
even
when
available.
Further,
find
analysis
mutations
observed
provide
signal
upcoming
lineage
transitions.
Our
demonstrates
utility
monitor
evolution
spread
endemic
viruses.
Water Research,
Journal Year:
2024,
Volume and Issue:
254, P. 121338 - 121338
Published: Feb. 18, 2024
Quantitative
polymerase
chain
reaction
(PCR)
and
genome
sequencing
are
important
methods
for
wastewater
surveillance
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
The
reverse
transcription-droplet
digital
PCR
(RT-ddPCR)
is
a
highly
sensitive
method
quantifying
SARS-CoV-2
RNA
in
samples
to
track
the
trends
viral
activity
levels
but
cannot
identify
new
variants.
It
also
takes
time
develop
PCR-based
assays
targeting
variants
interest.
Whole
(WGS)
can
be
used
monitor
known
variants,
it
generally
not
quantitative.
Several
short-read
techniques
expensive
might
experience
delayed
turnaround
times
when
outsourced
due
inadequate
in-house
resources.
Recently,
portable
nanopore
system
offers
an
affordable
real-time
wastewater.
This
technology
has
potential
enable
swift
response
disease
outbreaks
without
relying
on
clinical
results.
In
addressing
concerns
related
rapid
accurate
variant
analysis,
both
RT-ddPCR
were
employed
emergence
was
conducted
at
23
sewer
maintenance
hole
sites
five
treatment
plants
Michigan
from
2020
2022.
2020,
dominated
by
parental
(20A,
20C
20G),
followed
20I
(Alpha,
B.1.1.7)
early
2021
Delta
concern
(VOC)
late
2021.
For
year
2022,
Omicron
dominated.
Nanopore
validate
suspected
cases
that
initially
undetermined
assays.
concordance
rate
between
identifying
clade-level
76.9%.
Notably,
instances
disagreement
two
most
prominent
identification
We
showed
with
N
gene
concentrations
>104
GC/100ml
as
measured
improve
recovery
coverage
depth
using
MinION
device.
better
detecting
key
spike
protein
mutations
A67V,
del69-70,
K417N,
L452R,
N501Y,
N679K,
R408S
(p-value
<0.05)
compared
sequencing.
suggested
should
coordinated
where
preliminary
quantification
confirmatory
detection
or
reported
here
adopted
reliable
analysis
community
level
public
health
response.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: May 17, 2023
Abstract
As
clinical
testing
declines,
wastewater
monitoring
can
provide
crucial
surveillance
on
the
emergence
of
SARS-CoV-2
variant
concerns
(VoCs)
in
communities.
In
this
paper
we
present
QuaID,
a
novel
bioinformatics
tool
for
VoC
detection
based
quasi-unique
mutations.
The
benefits
QuaID
are
three-fold:
(i)
provides
up
to
3-week
earlier
detection,
(ii)
accurate
(>95%
precision
simulated
benchmarks),
and
(iii)
leverages
all
mutational
signatures
(including
insertions
&
deletions).
Heliyon,
Journal Year:
2024,
Volume and Issue:
10(9), P. e30600 - e30600
Published: May 1, 2024
Recently,
wastewater-based
epidemiology
(WBE)
research
has
experienced
a
strong
impetus
during
the
Coronavirus
disease
2019
(COVID-19)
pandemic.
However,
few
technical
issues
related
to
surveillance
strategies,
such
as
standardized
procedures
ranging
from
sampling
testing
protocols,
need
be
resolved
in
preparation
for
future
infectious
outbreaks.
This
review
highlights
study
characteristics,
potential
use
of
WBE
and
overview
methods,
well
methods
utilized
detect
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
including
its
variant
wastewater.
A
literature
search
was
performed
electronically
PubMed
Scopus
according
PRISMA
guidelines
relevant
peer-reviewed
articles
published
between
January
2020
March
2022.
The
identified
588
articles,
out
which
221
fulfilled
necessary
criteria
are
discussed
this
review.
Most
global
studies
were
conducted
North
America
(n=75,
34%),
followed
by
Europe
(n=68,
30.8%),
Asia
(n=43,
19.5%).
also
showed
that
most
application
observed
correlate
SARS-CoV-2
ribonucleic
acid
(RNA)
trends
sewage
with
epidemiological
data
(n=90,
40.7%).
techniques
often
used
globally
sample
collection,
concentration,
preferred
matrix
recovery
control
various
types
discussed.
Overall,
provided
framework
researchers
specializing
apply
strategic
approaches
their
questions
achieving
better
functional
insights.
In
addition,
areas
needed
more
in-depth
analysis,
ideas
new
initiatives
identified.
