High-Throughput Microfluidic Quantitative PCR Platform for the Simultaneous Quantification of Pathogens, Fecal Indicator Bacteria, and Microbial Source Tracking Markers DOI Creative Commons

Elizabeth R. Hill,

Chan Lan Chun, Kerry A. Hamilton

et al.

ACS ES&T Water, Journal Year: 2023, Volume and Issue: 3(8), P. 2647 - 2658

Published: July 24, 2023

Contamination of water with bacterial, viral, and protozoan pathogens can cause human diseases. Both humans nonhumans release these through their feces. To identify the sources fecal contamination in environment, microbial source tracking (MST) approaches have been developed; however, relationship between MST markers is still not well understood most likely due to lack comprehensive datasets marker concentrations. In this study, we developed a novel microfluidic quantitative PCR (MFQPCR) platform for simultaneous quantification 37 previously validated markers, two indicator bacteria (FIB), 22 11 five pathogens, three internal amplification/process controls many samples. The MFQPCR chip was applied analyze pathogen removal rates during wastewater treatment processes. addition, multiple host-specific FIB, were successfully quantified avian-impacted surface waters. While genes relatively infrequently detected, positive correlations observed some potential such as Clostridium perfringens Mycobacterium spp., markers. chips therefore, provide useful information monitor improve quality.

Language: Английский

Monkeypox outbreak: Wastewater and environmental surveillance perspective DOI Creative Commons
Ananda Tiwari, Sangeet Adhikari, Devrim Kaya

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 856, P. 159166 - 159166

Published: Oct. 3, 2022

Monkeypox disease (MPXD), a viral caused by the monkeypox virus (MPXV), is an emerging zoonotic endemic in some countries of Central and Western Africa but seldom reported outside affected region. Since May 2022, MPXD has been at least 74 globally, prompting World Health Organization to declare outbreak Public Emergency International Concern. As July 24, 2022; 92 % (68/74) with cases had no historical case reports. From One perspective, spread MPXV environment poses risk not only humans also small mammals may, ultimately, potent novel host populations. Wastewater-based surveillance (WBS) extensively utilized monitor communicable diseases, particularly during ongoing COVID-19 pandemic. It helped monitoring infectious caseloads as well specific variants circulating communities. The detection DNA lesion materials (e.g. skin, vesicle fluid, crusts), skin rashes, various body fluids, including respiratory nasal secretions, saliva, urine, feces, semen infected individuals, supports possibility using WBS early proxy for infections. can be used activity/trends sewerage network areas even before detecting laboratory-confirmed clinical within community. However, several factors affect wastewater including, limited to, routes duration time shedding infection rates relevant population, environmental persistence, processes analytical sensitivity methods. Further research needed identify key that impact biomarkers improve utility warning tool safeguarding human health. In this review, we shortly summarize aspects discuss challenges associated WBS.

Language: Английский

Citations

91
Tracing COVID-19 Trails in Wastewater: A Systematic Review of SARS-CoV-2 Surveillance with Viral Variants DOI Open Access
Ananda Tiwari, Sangeet Adhikari, Shuxin Zhang

et al.

Water, Journal Year: 2023, Volume and Issue: 15(6), P. 1018 - 1018

Published: March 7, 2023

The emergence of new variants SARS-CoV-2 associated with varying infectivity, pathogenicity, diagnosis, and effectiveness against treatments challenged the overall management COVID-19 pandemic. Wastewater surveillance (WWS), i.e., monitoring infections in communities through detecting viruses wastewater, was applied to track spread globally. However, there is a lack comprehensive understanding use WWS for variants. Here we systematically reviewed published articles reporting different wastewater by following PRISMA guidelines provided current state art this study area. A total 80 studies were found that reported until November 2022. Most these (66 out 80, 82.5%) conducted Europe North America, resource-rich countries. There high variation sampling strategy around world, composite (50/66 studies, 76%) as primary method In contrast, grab more common (8/14 57%) resource-limited Among detection methods, reverse transcriptase polymerase chain reaction (RT-PCR)-based sequencing quantitative RT-PCR commonly used wastewater. variants, B1.1.7 (Alpha) variant appeared earlier pandemic most (48/80 studies), followed B.1.617.2 (Delta), B.1.351 (Beta), P.1 (Gamma), others All same pattern clinical within timeline, demonstrating tracked all timely way when emerged. Thus, may be utilized identify presence or absence follow development transmission existing emerging Routine powerful infectious disease tool implemented

Language: Английский

Citations

40
Simultaneous detection of SARS-CoV-2, influenza A, respiratory syncytial virus, and measles in wastewater by multiplex RT-qPCR DOI Open Access
Emalie K. Hayes, Madison Gouthro, Jason J. LeBlanc

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 889, P. 164261 - 164261

Published: May 17, 2023

Language: Английский

Citations

35
Moving forward with COVID-19: Future research prospects of wastewater-based epidemiology methodologies and applications DOI Open Access
Guangming Jiang, Yanchen Liu, Song Tang

et al.

Current Opinion in Environmental Science & Health, Journal Year: 2023, Volume and Issue: 33, P. 100458 - 100458

Published: March 7, 2023

Language: Английский

Citations

29
Simultaneous detection and quantification of multiple pathogen targets in wastewater DOI Creative Commons
Gouthami Rao, Drew Capone, Kevin Zhu

et al.

PLOS Water, Journal Year: 2024, Volume and Issue: 3(2), P. e0000224 - e0000224

Published: Feb. 1, 2024

Wastewater-based epidemiology has emerged as a critical tool for public health surveillance, building on decades of environmental surveillance work pathogens such poliovirus. Work to date been limited monitoring single pathogen or small numbers in targeted studies; however, few studies consider simultaneous quantitative analysis wide variety pathogens, which could greatly increase the utility wastewater surveillance. We developed novel multi-pathogen approach (35 targets including bacteria, viruses, protozoa, and helminths) using TaqMan Array Cards (TAC) applied method concentrated samples collected at four treatment plants Atlanta, GA from February October 2020. From sewersheds serving approximately 2 million people, we detected range many expected find (e.g., enterotoxigenic E . coli Giardia 97% 29 stable concentrations) well unexpected Strongyloides stercoralis (a human threadworm rarely observed USA). Other notable detections included SARS-CoV-2, but also several that are not commonly like Acanthamoeba spp., Balantidium , Entamoeba histolytica astrovirus, norovirus, sapovirus. Our data suggest broad expanding scope enteric wastewaters, with potential application settings where quantification fecal waste streams can inform selection control measures limit infections.

Language: Английский

Citations

15
Evaluation of plasmid pBI143 for its optimal concentration methods, seasonal impact, and potential as a normalization parameter in wastewater-based epidemiology DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 965, P. 178661 - 178661

Published: Feb. 1, 2025

Language: Английский

Citations

1
Estimation of Norovirus infections in Japan: An application of wastewater-based epidemiology for enteric disease assessment DOI
Sadhana Shrestha, Bikash Malla, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 169334 - 169334

Published: Dec. 14, 2023

Language: Английский

Citations

21
Wastewater surveillance of Mpox virus in Baltimore DOI Open Access
Samendra P. Sherchan, Tamunobelema Solomon,

Oladele Idris

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 891, P. 164414 - 164414

Published: May 23, 2023

Language: Английский

Citations

20
Optimization of the 5-plex digital PCR workflow for simultaneous monitoring of SARS-CoV-2 and other pathogenic viruses in wastewater DOI
Bikash Malla, Sadhana Shrestha, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 913, P. 169746 - 169746

Published: Dec. 28, 2023

Language: Английский

Citations

19
Long-term SARS-CoV-2 surveillance in wastewater and estimation of COVID-19 cases: An application of wastewater-based epidemiology DOI Creative Commons
Sadhana Shrestha, Bikash Malla, Made Sandhyana Angga

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 896, P. 165270 - 165270

Published: July 1, 2023

The role of wastewater-based epidemiology (WBE), a powerful tool to complement clinical surveillance, has increased as many grassroots-level facilities, such municipalities and cities, are actively involved in wastewater monitoring, the testing coronavirus disease 2019 (COVID-19) is downscaled widely. This study aimed conduct long-term surveillance severe acute respiratory syndrome 2 (SARS-CoV-2) Yamanashi Prefecture, Japan, using one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay estimate COVID-19 cases cubic regression model that simple implement. Influent samples (n = 132) from treatment plant were collected normally once weekly between September 2020 January 2022 twice February August 2022. Viruses (40 mL) concentrated by polyethylene glycol precipitation method, followed RNA extraction RT-qPCR. K-6-fold cross-validation method was used select appropriate data type (SARS-CoV-2 concentration cases) suitable for final run. SARS-CoV-2 successfully detected 67 % (88 tested during whole period, 37 (24 65) 96 (64 67) before 2022, respectively, with concentrations ranging 3.5 6.3 log10 copies/L. applied nonnormalized nonstandardized running 14-day (1 14 days) offset models average cases. Comparing parameters evaluation, best showed lagged 3 days behind Omicron variant phase (year 2022). Finally, 3- 7-day predicted trend until 2023, indicating applicability WBE an early warning tool.

Language: Английский

Citations

18