No impacts of glyphosate or Crithidia bombi, or their combination, on the bumblebee microbiome

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: June 2, 2023

Pesticides are recognised as a key threat to pollinators, impacting their health in many ways. One route through which pesticides can affect pollinators like bumblebees is the gut microbiome, with knock-on effects on immune system and parasite resistance. We tested impacts of high acute oral dose glyphosate microbiome buff tailed bumblebee (Bombus terrestris), glyphosate's interaction (Crithidia bombi). used fully crossed design measuring bee mortality, intensity bacterial composition estimated from relative abundance 16S rRNA amplicons. found no impact either glyphosate, C. bombi, or combination any metric, including composition. This result differs studies honeybees, have consistently an potentially explained by use exposure, rather than chronic difference test species. Since A. mellifera model species represent more broadly risk assessment, our results highlight that caution needed extrapolating other

Language: Английский

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Effects of glyphosate and glyphosate‐based herbicide on learning and memory of the buff‐tailed bumblebee (Bombus terrestris)

Entomologia Experimentalis et Applicata, Journal Year: 2024, Volume and Issue: 172(4), P. 324 - 333

Published: Feb. 3, 2024

Abstract The decline of insect pollinators is a global concern, and the use pesticides has been identified as potential cause for it. Glyphosate‐based herbicides (GBHs) are world's most used pesticides, but until recent years they have claimed to be safe non‐target organisms, such pollinators. Using controlled arena experiments, we investigated effects on learning long‐term memory buff‐tailed bumblebees, Bombus terrestris (L.) (Hymenoptera: Apidae), single field‐realistic dose glyphosate, both in its pure form commercial herbicide (Roundup Gold) containing active ingredient (a.i.) glyphosate other co‐formulants. We found that caused deterioration ability bumblebees 10‐color discrimination experiment; glyphosate‐treated bees discriminated colors over 10% worse than untreated control bees. However, commercially GBH had no observable effect despite fact this contained same amount form. These findings shed light risks associated with agrochemicals previously considered emphasize need comprehensive risk assessments including evaluations cognitive functions Therefore, propose future pesticide testing should incorporate broader ensure safety agricultural landscapes.

Language: Английский

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Glyphosate resistance and biodegradation by Burkholderia cenocepacia CEIB S5-2

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(25), P. 37480 - 37495

Published: May 22, 2024

Language: Английский

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Changes in bumblebee queen gut microbiotas during and after overwintering diapause

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Bumblebees are key pollinators with gut microbiotas that support host health. After bumblebee queens undergo winter diapause, which occurs before spring colony establishment, their disturbed, but little is known about community dynamics during diapause itself. Queen also help seed worker microbiotas, so it important they recover post‐diapause to a typical structure, process may be impeded by pesticide exposure. We examined how queen microbiota structure and metabolic potential shift after whether recovery affected To do so, we placed commercial Bombus impatiens into euthanizing them at 0, 2 4 months of diapause. Additionally, allowed some from for 1 week euthanasia, exposing half the common herbicide glyphosate. Using whole‐community, shotgun metagenomic sequencing, found core bee phylotypes dominated before, two phylotypes, Schmidhempelia Snodgrassella , ceased detected late recovery. Despite fluctuations in taxonomic remained constant through Also, glyphosate exposure did not affect However, assembly quality our ability detect microbial taxa pathways declined alongside abundance, was substantially reduced Our study offers new insights change taxonomically functionally life stage provides guidance future studies diapausing bumblebees.

Language: Английский

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Caffeine Consumption Helps Honey Bees Fight a Bacterial Pathogen

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(3)

Published: May 22, 2023

The consumption of caffeine is a remarkable feature the human diet. Common drinks, such as coffee and tea, contain stimulant.

Language: Английский

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A mixture of mesotrione and atrazine harms adults and larvae of the predatory wasp Polistes satan

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 923, P. 171526 - 171526

Published: March 7, 2024

Language: Английский

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Colony performance of three native bumblebee species from South China and association with their gut microbiome

Insect Science, Journal Year: 2024, Volume and Issue: 31(6), P. 1960 - 1983

Published: March 22, 2024

Abstract Bumblebees play an important ecological economic role as pollinators in nature and agriculture. For reasons of biosecurity, many countries promote the cultivation native bumblebee species for crop pollination instead importing “alien” species. In South China, a few are considered useful this way, particularly, Bombus atripes , bicoloratus breviceps . However, whether they suitable artificial rearing forming healthy colonies pollination, remains unknown. project, queens from 3 Guizhou Province were collected started under standardized conditions. The scored based on 19 parameters, including stage colony development, number weight offspring, diet consumed. data revealed that B. had best performance, produced more workers consumed smallest diet. Next, we performed 16S rDNA sequencing bacterial communities found guts offspring workers, then correlation analysis between performance gut bacteria was conducted. Here, showed highest diversity composition, dominated by Gilliamella Snodgrassella Enterobacter Lactobacillus Firm5. higher abundance better foundation stage, later Firm5, Apibacter Bifidobacterium beneficial during stages rapid growth decline. Although do not understand all interactions yet, these correlations explain why demonstrated performance. Our provide valuable information breeding local will contribute to developing strong pollination.

Language: Английский

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Glyphosate effects on growth and biofilm formation in bee gut symbionts and diverse associated bacteria

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(8)

Published: Aug. 21, 2024

Biofilm formation is a common adaptation enabling bacteria to thrive in various environments and withstand external pressures. In the context of host-microbe interactions, biofilms play vital roles establishing microbiomes associated with animals plants are used by opportunistic microbes facilitate survival within hosts. Investigating biofilm dynamics, composition, responses environmental stressors crucial for understanding microbial community assembly regulation health disease. this study, we explore

Language: Английский

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Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: Aug. 7, 2024

Abstract Background In this study, a probiotic mixture (Honeybeeotic) consisting of seven bacterial strains isolated from unique population honeybees ( Apis mellifera ligustica ) was used. That honeybee located in the Roti Abbey locality Marche Region Italy, an area human activities, and genetic contamination other populations. The aim to investigate effects on innate immunity intestinal microbiome healthy common two hives same apiary. Hive A received diet 50% glucose syrup, while hive B syrup supplemented with probiotics, both administered daily for 1 month. To determine whether altered immune response, phenoloxidase activity hemolymph cellular subtype count were investigated. Additionally, metagenomic approaches used analyze gut microbiota composition function, considering critical role plays modulating host physiology. Results results revealed differences hemocyte populations between hives, as exhibited higher counts oenocytoids granulocytes. These findings indicated that dietary supplementation safe well-tolerated. Furthermore, significantly decreased (1.75 ± 0.19 U/mg) compared (3.62 0.44 U/mg, p < 0.005), suggesting improved state well-being honeybees, they did not require activation defense mechanisms. Regarding composition, modulated control, retaining core components causing positive negative variations. Notably, several genes, particularly KEGG genes involved amino acid metabolism, carbohydrate branched-chain (BCAA) transport, more abundant probiotic-fed group, effective nutritional supplement host. Conclusions This study advocated feeding induces beneficial immunological promoted balanced enhanced metabolic activities related digestion. use highly selected probiotics shown contribute overall improving their response health.

Language: Английский

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Effects of pesticide-adjuvant combinations used in almond orchards on olfactory responses to social signals in honey bees (Apis mellifera)

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 20, 2023

Abstract Exposure to agrochemical sprays containing pesticides and tank-mix adjuvants has been implicated in post-bloom mortality, particularly of brood, honey bee colonies brought into California almond orchards for pollination. Although are generally considered be biologically inert, some have exhibited toxicity sublethal effects, including decreasing survival rates next-generation queens. Honey bees a highly developed olfactory system detect discriminate among social signals. To investigate the impact pesticide-adjuvant combinations on signal perception, we performed electroantennography assays assess alterations their responsiveness brood ester pheromone (BEP), volatile larval β-ocimene, alarm 2-heptanone. These aimed uncover potential mechanisms underlying changes behaviors reduced after pesticide exposure. We found that combining adjuvant Dyne-Amic with fungicide Tilt (propiconazole) insecticide Altacor (chlorantraniliprole) synergistically enhanced responses three concentrations BEP as well exerted dampening compensatory effects 2-heptanone respectively. In contrast, exposure alone or combination had no effect at most but altered β-ocimene Dyne-Amic, Altacor, increased amplitude, indicating receptor sensitivity sensilla permeability odorants. Given that, previous study, queens raised by nurses exposed same treated pollen experienced survival, these new findings highlight disruption signaling its implications colony reproductive success.

Language: Английский

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