medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 27, 2023
Abstract
Central
Michigan
University
(CMU)
participated
in
a
state-wide
SARS-CoV-2
wastewater
monitoring
program
since
2021.
Wastewater
samples
were
collected
from
on-campus
sites
and
nine
off-campus
treatment
plants
servicing
small
metropolitan
rural
communities.
genome
copies
quantified
using
droplet
digital
PCR
results
reported
to
the
health
department.
One
rural,
site
consistently
produced
higher
concentrations
of
copies.
Samples
this
sequenced
initially
contained
predominately
Alpha
variant
lineage
Q.3,
which
transitioned
Q.4.
Q.3/Q.4
was
detected
at
beginning
fall
2021
continued
until
summer
2023.
Mutational
analysis
reconstructed
genes
revealed
divergence
Q.3
clinical
sequence
over
time,
including
numerous
mutations
surface
glycoprotein
RBD
NTD.
We
discuss
possibility
that
chronic
infection
accumulated
adaptive
promoted
long-term
infection.
This
study
reveals
can
enhance
resolution
rare
events
facilitate
reconstruction
viral
genomes
due
relative
lack
contaminating
sequences.
Clinical Microbiology Reviews,
Journal Year:
2023,
Volume and Issue:
37(1)
Published: Dec. 14, 2023
SUMMARY
Wastewater-based
surveillance
(WBS)
has
undergone
dramatic
advancement
in
the
context
of
coronavirus
disease
2019
(COVID-19)
pandemic.
The
power
and
potential
this
platform
technology
were
rapidly
realized
when
it
became
evident
that
not
only
did
WBS-measured
SARS-CoV-2
RNA
correlate
strongly
with
COVID-19
clinical
within
monitored
populations
but
also,
fact,
functioned
as
a
leading
indicator.
Teams
from
across
globe
innovated
novel
approaches
by
which
wastewater
could
be
collected
diverse
sewersheds
ranging
treatment
plants
(enabling
community-level
surveillance)
to
more
granular
locations
including
individual
neighborhoods
high-risk
buildings
such
long-term
care
facilities
(LTCF).
Efficient
processes
enabled
extraction
concentration
highly
dilute
matrix.
Molecular
genomic
tools
identify,
quantify,
characterize
its
various
variants
adapted
programs
applied
these
mixed
environmental
systems.
Novel
data-sharing
allowed
information
mobilized
made
immediately
available
public
health
government
decision-makers
even
public,
enabling
evidence-informed
decision-making
based
on
local
dynamics.
WBS
since
been
recognized
tool
transformative
potential,
providing
near-real-time
cost-effective,
objective,
comprehensive,
inclusive
data
changing
prevalence
measured
analytes
space
time
populations.
However,
consequence
rapid
innovation
hundreds
teams
simultaneously,
tremendous
heterogeneity
currently
exists
literature.
This
manuscript
provides
state-of-the-art
review
established
details
current
work
underway
expanding
scope
other
infectious
targets.
Water Research,
Journal Year:
2024,
Volume and Issue:
261, P. 122024 - 122024
Published: June 30, 2024
Water
quality,
critical
for
human
survival
and
well-being,
necessitates
rigorous
control
to
mitigate
contamination
risks,
particularly
from
pathogens
amid
expanding
urbanization.
Consequently,
the
necessity
maintain
microbiological
safety
of
water
supplies
demands
effective
surveillance
strategies,
reliant
on
collection
representative
samples
precise
measurement
contaminants.
This
review
critically
examines
advancements
passive
sampling
techniques
monitoring
in
various
systems,
including
wastewater,
freshwater,
seawater.
We
explore
evolution
conventional
materials
innovative
adsorbents
pathogen
capture
shift
culture-based
molecular
detection
methods,
underscoring
adaptation
this
field
global
health
challenges.
The
comparison
highlights
sampling's
efficacy
over
like
grab
its
potential
overcome
existing
challenges
through
use
such
as
granular
activated
carbon,
thermoplastics,
polymer
membranes.
By
evaluating
literature,
work
identifies
standardization
gaps
proposes
future
research
directions
augment
efficiency,
specificity,
utility
environmental
public
surveillance.
BMC Genomics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: Jan. 13, 2024
Central
Michigan
University
(CMU)
participated
in
a
state-wide
SARS-CoV-2
wastewater
monitoring
program
since
2021.
Wastewater
samples
were
collected
from
on-campus
sites
and
nine
off-campus
treatment
plants
servicing
small
metropolitan
rural
communities.
genome
copies
quantified
using
droplet
digital
PCR
results
reported
to
the
health
department.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
917, P. 170367 - 170367
Published: Jan. 24, 2024
Global
efforts
in
vaccination
have
led
to
a
decrease
COVID-19
mortality
but
high
circulation
of
SARS-CoV-2
is
still
observed
several
countries,
resulting
some
cases
severe
lockdowns.
In
this
sense,
wastewater-based
epidemiology
remains
powerful
tool
for
supporting
regional
health
administrations
assessing
risk
levels
and
acting
accordingly.
work,
dynamic
artificial
neural
network
(DANN)
has
been
developed
predicting
the
number
hospitalized
patients
hospitals
Valladolid
(Spain).
This
model
takes
as
inputs
wastewater
indicator
(concentration
RNA
from
N1
gene
reported
Wastewater
Treatment
Plant),
coverage,
past
data
hospitalizations.
The
considered
both
instantaneous
values
these
variables
their
historical
evolution.
Two
study
periods
were
selected
(from
May
2021
until
September
2022
July
2023).
During
first
period,
accurate
predictions
hospitalizations
(with
an
overall
range
between
6
171)
favored
by
correlation
with
concentrations
(r
=
0.43,
p
<
0.05),
showing
forecasting
1
day
ahead
5
days
ahead.
second
period's
retraining
strategy
maintained
accuracy
despite
lower
Furthermore,
assigned
each
prediction
during
periods,
agreement
level
measured
authorities
95
%
93
cases,
respectively.
These
results
evidenced
potential
novel
DANN
based
on
at
scale.
architecture
herein
can
support
management
epidemiology.
Human Genomics,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Feb. 1, 2024
Abstract
Background
Human
viruses
released
into
the
environment
can
be
detected
and
characterized
in
wastewater.
The
study
of
wastewater
virome
offers
a
consolidated
perspective
on
circulation
within
population.
Because
occurrence
severity
viral
infections
vary
across
person’s
lifetime,
studying
samples
contributed
by
various
demographic
segments
provide
valuable
insights
prevalence
these
segments.
In
our
study,
targeted
enrichment
sequencing
was
employed
to
characterize
human
at
building-level
scale.
This
accomplished
through
passive
sampling
schools,
university
settings,
nursing
homes
two
cities
Catalonia.
Additionally,
sewage
from
large
urban
treatment
plant
analysed
serve
as
reference
for
examining
collective
excreted
virome.
Results
obtained
influent
showcased
combined
presence
individuals
varying
ages,
with
astroviruses
bocaviruses
being
most
prevalent,
followed
adenoviruses,
polyomaviruses,
papillomaviruses.
Significant
variations
profiles
were
observed
among
different
types
buildings
studied.
Mamastrovirus
1
predominant
school
samples,
salivirus
polyomaviruses
JC
BK
settings
while
showed
more
balanced
distribution
families
presenting
papillomavirus
picornaviruses
and,
interestingly,
some
linked
immunosuppression.
Conclusions
shows
utility
wastewater-based
epidemiology
an
effective
tool
monitoring
circulating
specific
age
groups.
It
provides
public
health
epidemiological
studies.
Water Research,
Journal Year:
2023,
Volume and Issue:
244, P. 120469 - 120469
Published: Aug. 8, 2023
Wastewater-based
surveillance
(WBS)
has
been
established
as
a
powerful
tool
that
can
guide
health
policy
at
multiple
levels
of
government.
However,
this
approach
not
well
assessed
more
granular
scales,
including
large
work
sites
such
University
campuses.
Between
August
2021
and
April
2022,
we
explored
the
occurrence
SARS-CoV-2
RNA
in
wastewater
using
qPCR
assays
from
complimentary
sewer
catchments
residential
buildings
spanning
Calgary's
campus
how
compared
to
municipal
treatment
plant
servicing
campus.
Real-time
contact
tracing
data
was
used
evaluate
an
association
between
burden
clinically
confirmed
cases
assess
potential
WBS
for
disease
monitoring
across
worksites.
Concentrations
N1
N2
varied
significantly
six
sampling
-
regardless
several
normalization
strategies
with
certain
consistently
demonstrating
values
1-2
orders
higher
than
others.
Relative
clinical
identified
specific
sewersheds,
provided
one-week
leading
indicator.
Additionally,
our
comprehensive
strategy
enabled
estimation
total
per
capita,
which
lower
surrounding
community
(p≤0.001).
Allele-specific
variants
were
representative
large,
no
time
did
emerging
first
debut
on
This
study
demonstrates
be
efficiently
applied
locate
hotspots
activity
very
scale,
predict
complex
SSM - Population Health,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101786 - 101786
Published: March 1, 2025
Population
health
data
from
wastewater-based
epidemiology
(WBE)
are
being
used
at
unprecedented
scales
worldwide,
yet
there
is
limited
focus
on
how
to
advance
equity
in
the
field.
Addressing
this
gap,
we
conducted
a
critical
review
of
published
literature
PubMed,
targeting
studies
intersection
WBE
and
equity.
Of
145
articles
assessed
full-text
screening,
identified
68
with
considerations.
These
spanned
various
spatial
biochemical
targets,
addressing
domains
such
as
study
design
methodologies,
ethical
social
considerations,
feasibility
implementation
monitoring.
We
summarize
synthesize
equity-oriented
considerations
across
domains.
further
propose
five
key
research
practice,
integrate
these
into
conceptual
framework
illustrate
they
apply
major
steps
process
conducting
WBE.
include
global
inequities
access,
need
prevent
potential
harms
stigma
via
misuse
(inappropriate
reporting
use
for
criminal
surveillance),
importance
regulation
community
engagement,
particularly
amidst
growing
privatization
WBE,
especially
United
States.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
957, P. 177449 - 177449
Published: Nov. 19, 2024
Wastewater-based
epidemiology
(WBE)
is
an
effective,
non-invasive
method
for
monitoring
the
spread
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
by
tracking
viral
prevalence
in
water.
This
study
aimed
to
investigate
presence
SARS-CoV-2
surface
water
Vietnam
over
two
years.
One-step
quantitative
reverse
transcription
polymerase
chain
reaction
(qRT-PCR)
assays
were
employed
quantify
and
its
variant-specific
mutation
sites
(G339D/E484A)
pepper
mild
mottle
virus
(PMMoV)
from
a
total
315
samples
(105
per
type)
compare
with
reported
Coronavirus
disease
2019
(COVID-19)
cases
environmental
factors.
was
detected
38
%
(40/105),
43
(45/105),
39
(41/105)
Sites
A,
B,
C,
respectively,
concentrations
3.0-5.6
log
