Co-culturing a multistrain Gram-negative inoculant useful in sustainable agriculture DOI Creative Commons
Beatrice Farda, Fabrizia Pasquarelli, Rihab Djebaili

et al.

Frontiers in Industrial Microbiology, Journal Year: 2024, Volume and Issue: 2

Published: March 26, 2024

Microbial-based inoculants for agricultural use consist of different strains. The consortia production process involves growing pure cultures separately and combining them in the appropriate ratio. However, inclusion multiple strains formulation increases costs. By developing co-culture fermentations, it is possible to create with correct bacterial charge. This study aims develop a cost-effective approach producing an inoculum that includes ratio four Gram-negative bacteria, i.e., Azospirillum brasilense , Burkholderia ambifaria Gluconacetobacter diazotrophicus Herbaspirillum seropedicae . specific growth rates were studied using T4 medium, previously optimized their culture. co-fermentation was 500 mL flasks attain equivalent density 9.7-10 Log CFU -1 Then, successfully scaled up 5 L bioreactor, obtaining 9.7-9.9 first co-formulation multistrain consortium formed by plant growth-promoting bacteria pave road future evaluations other products useful sustainable agriculture.

Language: Английский

Coupling Waste Feedstocks to Microbial Protein Production in a Circular Food System DOI
Taylor Uekert, Alissa Bleem, Eric C. Holmes

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Global food production is a major contributor to greenhouse gas emissions, water consumption, and land use. As an alternative conventional agriculture, the of waste-derived microbial protein (MP) holds promise for reducing environmental impacts. MP can be mass-produced in volumetrically scalable fermentation processes on short time scales, enabling facile scale-up with lower use, impacts than animal and, some cases, plant protein. also produced from waste feedstocks, diverting landfills or natural environment. This Perspective explores availability suitability feedstocks production, suggesting that generated United States could fulfill twice current national demand. We discuss biotechnological separations required produce food-grade human consumption waste. Key challenges include consistency, consumer regulatory acceptance, process utilities (electricity, heat, nutrients) account up 85% costs most impacts, all which present opportunities innovation microbiology design spaces. Overall, this work highlights potential contribute more circular, resilient, sustainable system.

Language: Английский

Citations

2

Predicting biomass conversion and COD removal in wastewater treatment by phototrophic bacteria with interpretable machine learning DOI
Pengfei Hou, Shiqi Liu,

Duofei Hu

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 375, P. 124282 - 124282

Published: Jan. 24, 2025

Language: Английский

Citations

2

From anaerobic digestion to single cell protein synthesis: A promising route beyond biogas utilization DOI
Rui Li,

Xiaolei Fan,

Yufeng Jiang

et al.

Water Research, Journal Year: 2023, Volume and Issue: 243, P. 120417 - 120417

Published: July 26, 2023

Language: Английский

Citations

27

Microbial protein sources: A comprehensive review on the potential usage of fungi and cyanobacteria in sustainable food systems DOI
Nagarjuna Prakash Dalbanjan,

Manjunath P. Eelager,

Shivayogi S. Narasagoudr

et al.

Food and Humanity, Journal Year: 2024, Volume and Issue: 3, P. 100366 - 100366

Published: July 27, 2024

Language: Английский

Citations

16

Bacterial single cell protein (BSCP): A sustainable protein source from methylobacterium species DOI Creative Commons
Marttin Paulraj Gundupalli, Sara Ansari, Jaquelinne Pires Vital da Costa

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 147, P. 104426 - 104426

Published: March 23, 2024

Single Cell Protein (SCP) is a potential replacement for traditional protein sources in both food and feed. This review specifically focuses on SCP produced by bacteria, emphasizing its unique advantages such as rapid growth, high content, efficient manufacturing, genetic flexibility, thorough strain characterization. Notably, among other microorganisms, Methylobacterium species have recently been found to be crucial synthesis. The examines the feasibility of incorporating sustainable substitute diets aquaculture cattle production closely analyzing fishmeal soybean meal. Additionally, it highlights importance ongoing research supportive legislation, while also considering economic policy factors assess financial viability production. concludes outlining future prospects outlook derived from species, providing forward-looking perspective evolving applications SCP.

Language: Английский

Citations

8

Research progress on cottonseed meal as a protein source in pig nutrition: An updated review DOI Creative Commons

An Tao,

Jiahao Wang,

Bin Luo

et al.

Animal nutrition, Journal Year: 2024, Volume and Issue: 18, P. 220 - 233

Published: May 25, 2024

At a global level, the supply of protein sources is insufficient to support current magnitude pig production. Moreover, given exorbitant expense conventional feed options like soybean meal and fish meal, it becomes imperative promptly explore alternative for sustainable advancement industry. Cottonseed by-product from extraction cottonseed oil, exhibits significant potential as source owing its high content, yield, low cost, well-balanced amino acid composition, sufficient accessibility. However, possesses several anti-nutritional factors, especially gossypol, which adversely affect growth reproductive performance, resulting in limited utilization feed. To maximize benefits promote application production, acquire comprehensive knowledge regarding nutritional value utilization. In this review, we initially presented summary values primary effective approaches improving Subsequently, comprehensively summarized latest research progress nutrition over past decade. The outcome review serves theoretical foundation practical guidance promotes reduction

Language: Английский

Citations

5

Research Progress in Printing Formulation for 3D Printing of Healthy Future Foods DOI
Anjelina Sundarsingh, Min Zhang, Arun S. Mujumdar

et al.

Food and Bioprocess Technology, Journal Year: 2023, Volume and Issue: 17(11), P. 3408 - 3439

Published: Dec. 13, 2023

Language: Английский

Citations

12

Power-to-Protein: Electro-cultivation of microbial proteins from recycled nitrogen and carbon dioxide DOI
Laura Rovira-Alsina, Narcís Pous, M. Dolors Balaguer

et al.

Journal of Power Sources, Journal Year: 2025, Volume and Issue: 638, P. 236499 - 236499

Published: Feb. 27, 2025

Language: Английский

Citations

0

Sustainable Animal Husbandry and Reduced Greenhouse Gas Emissions Through Single-Cell Protein DOI
H. Gado, Mona M. M. Y. Elghandour, Abdelfattah Z.M. Salem

et al.

Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 50

Published: Jan. 1, 2025

Language: Английский

Citations

0

Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer DOI Open Access
Tawakalt Ayodele,

Kudirat Alarape,

Ibrahim Bello

et al.

Sustainability, Journal Year: 2024, Volume and Issue: 16(13), P. 5486 - 5486

Published: June 27, 2024

The expanding global population has increased the demand for sustainable protein sources, and microbial (MP) emerged as a promising alternative. However, conventional carbon (glucose) nitrogen (ammonia, urea) sources needed MP production pose environmental economic issues. This study aims to produce using lignocellulosic biomass (LCB) source fixation ability of Klebsiella oxytoca M5A1 source. investigates pretreatment LCB (switchgrass), enzymatic hydrolysis, quantification, fixation, glucose utilization organic acids production. K. harnessed free from atmosphere used abundant, cheap by-products. Protein occurred in two phases: first within initial 8 h secondly, last 16 h. highest concentration was at 40 h, with approximately 683.46 µg/mL content. High-performance liquid chromatography system (HPLC) analysis revealed dynamic profile (Lactic acid, Propionic Acetic Succinic acid) exhibited an early high rate consumption, conversion acids, that were later second-phase intra-conversion one acid another via metabolic pathways (glycolysis tricarboxylic cycle). Overall, leveraging nitrogen-fixing offers eco-friendly cost-effective alternative traditional contributing circular economy.

Language: Английский

Citations

2