Engineered exosomes-based theranostic strategy for tumor metastasis and recurrence

Asian Journal of Pharmaceutical Sciences, Journal Year: 2023, Volume and Issue: 18(6), P. 100870 - 100870

Published: Nov. 1, 2023

Metastasis-associated processes are the predominant instigator of fatalities linked to cancer, wherein pivotal role circulating tumor cells lies in resurgence malignant growth. In recent epochs, exosomes, constituents extracellular vesicle cohort, have garnered attention within field theranostics owing their inherent attributes encompassing biocompatibility, modifiability, payload capacity, stability, and therapeutic suitability. Nonetheless, rudimentary functionalities limited efficacy unmodified exosomes curtail prospective utility. an effort surmount these shortcomings, intricate methodologies amalgamating nanotechnology with genetic manipulation, chemotherapy, immunotherapy, optical intervention present themselves as enhanced avenues surveil intercede metastasis relapse. This review delves into manifold techniques currently employed engineer a specific focus on elucidating interplay between metastatic cascade, alongside implementation tailored abating recurrence. not only advances comprehension evolving landscape this domain but also steers trajectory forthcoming investigations.

Language: Английский

---

Lung Fibroblasts Take up Breast Cancer Cell-derived Extracellular Vesicles Partially Through MEK2-dependent Macropinocytosis

Cancer Research Communications, Journal Year: 2024, Volume and Issue: 4(1), P. 170 - 181

Published: Jan. 22, 2024

Abstract Extracellular vesicles (EV) have emerged as critical effectors in the cross-talk between cancer and normal cells by transferring intracellular materials adjacent or distant cells. Previous studies begun to elucidate how cells, secreting EVs, adapt at a metastatic site facilitate cell metastasis. In this study, we utilized high-content microscopic screening platform investigate mechanisms of EV uptake primary lung fibroblasts. A selected library containing 90 FDA-approved anticancer drugs was screened for effect on fibroblast EVs from MDA-MB-231 breast Among identified inhibit without exerting significant cytotoxicity, validated dose-dependent Trametinib (a MEK1/2 inhibitor) Copanlisib PI3K inhibitor). suppressed macropinocytosis fibroblasts inhibited with higher potency comparing Copanlisib. Gene knockdown overexpression demonstrated that required MEK2. These findings provide important insights into underlying cell-derived which could play role metastasis lungs suggest potential therapeutic targets preventing treating deadly disease. Significance: Through phenotypic screen, found MEK inhibitor fibroblasts, is mediated MEK2 these Our results inhibition serve strategy block

Language: Английский

---

The role of extracellular vesicles in cancer

Current topics in membranes, Journal Year: 2024, Volume and Issue: unknown, P. 247 - 285

Published: Jan. 1, 2024

Language: Английский

---

Neutrophil Membrane Nanovesicles Alleviate the Renal Function Indicators in Acute Kidney Injury Caused by Septic Rats

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Language: Английский

---

Potential and development of cellular vesicle vaccines in cancer immunotherapy

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 15, 2025

Cancer vaccines are promising as an effective means of stimulating the immune system to clear tumors well establish surveillance. In this paper, we discuss main platforms and current status cancer propose a new vaccine platform, cytosolic vesicle vaccine. This has unique structure that can integrate antigen adjuvant carriers improve delivery efficiency activation ability, which brings ideas for design. Tumor exosomes carry antigens MHC-peptide complexes, provide tumor antigen-processing cells increase chances recognition by cells. DEVs play role in amplifying response acting dissemination antigenic substances dendritic OMVs, with their natural properties, one advantages preparation antitumor vaccines. paper presents these three bacteria/extracellular vesicles discusses potential applications functionally modified extracellular after cellular engineering or genetic engineering, clinical trials summary, direction research.

Language: Английский

---

Cancer-secreted exosomal miR-1825 induces angiogenesis to promote colorectal cancer metastasis

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 22, 2025

Angiogenesis is one of the important factors related to tumorigenesis, invasion, and metastasis. Cancer-secreted exosomes are essential mediators intercellular cross-talk participate in angiogenesis Unveiling mechanism an way develop anti-angiogenesis therapeutic strategies against cancer progression. miR-1825 expression relationship with microvascular density were validated colorectal (CRC) by situ hybridization (ISH) staining immunohistochemistry (IHC). Sequential differential centrifugation, transmission electron microscopy, western blotting analysis used extract characterize exosomes. The effort exosomal on endothelial cells was examined transwell assay, wound healing tube formation aortic ring assay. miR-1825, ING1 downstream pathway analyzed blot, RT-PCR, Immunofluorescence, dual-luciferase reporter system analysis. Exosomal associated CRC enriched extracted from serum patients. CRC-secreted can be transferred into vascular cells, promoting cell migration vitro, facilitating tumor metastasis vivo. Mechanistically, regulates suppressing inhibitor growth family member 1 (ING1) activating TGF-β/Smad2/Smad3 signaling recipient HUVECs. Our study demonstrated could subsequently leads inhibition activation pathway, thereby liver CRC. thus a potential diagnostic target for

Language: Английский

---

hnRNPA2B1 facilitates ovarian carcinoma metastasis by sorting cargoes into small extracellular vesicles driving myofibroblasts activation

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 4, 2025

Ovarian carcinoma (OvCa) metastasis is initiated and boosted by tumor-stroma interactions mediated small extracellular vesicles (sEVs) containing microRNAs (miRNAs). However, the mechanisms of sorting relevant miRNAs into tumoral sEVs remain elusive. In this study, among RNA-binding proteins, hnRNPA2B1 was identified as most significant factor associated with survival in OvCa patients, its expression higher omental metastases compared to paired ovarian lesions. Based on CRISPR-Cas9 technique, orthotopic xenograft mice revealed a remarkable metastasis-inhibiting effect hnRNPA2B1-knockdown, accompanied diminished myofibroblast signals omentum. Meanwhile, after OvCa-sEVs largely lost ability promote activation vivo vitro. High-throughput miRNA sequencing sEV cargoes that UAG motif-containing were significantly affected hnRNPA2B1, RNA immunoprecipitation (RIP) verified their direct binding hnRNPA2B1. pull down assays, mutated motif exhibited decreased capacity The myofibroblasts activated could tumor metastasis, notably impacted manipulating related sEV-miRNAs, PI3K/AKT signaling. These findings highlight an important mechanism involved which may illuminate new therapeutic strategies.

Language: Английский

---

Pathological and Therapeutic Significance of Tumor-Derived Extracellular Vesicles in Cancer Cell Migration and Metastasis

Cancers, Journal Year: 2023, Volume and Issue: 15(18), P. 4425 - 4425

Published: Sept. 5, 2023

The infiltration of primary tumors and metastasis formation at distant sites strongly impact the prognosis quality life cancer patients. Current therapies including surgery, radiotherapy, chemotherapy are limited in targeting complex cell migration mechanisms responsible for invasiveness metastasis. A better understanding these development new urgently needed. Extracellular vesicles (EVs) lipid-enveloped particles involved inter-tissue inter-cell communication. This review article focuses on EVs released by tumor cells, specifically We first introduce processes EV subtypes, we give an overview how tumor-derived (TDEVs) may migration. Then, discuss ongoing EV-based therapeutic approaches, inhibition general EV-related as well use anti-cancer drug delivery, focusing harnessing TDEVs. propose a protein-EV shuttle route alternative to secretion or membrane binding, influencing downstream signaling final effect target with strong implications tumorigenesis. Finally, highlight pitfalls limitations exploitation that must be overcome realize promise therapy.

Language: Английский

---

Advancements in melanoma immunotherapy: the emergence of Extracellular Vesicle Vaccines

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Aug. 23, 2024

Abstract Malignant melanoma represents a particularly aggressive type of skin cancer, originating from the pathological transformation melanocytes. While conventional interventions such as surgical resection, chemotherapy, and radiation therapy are available, their non-specificity collateral damage to normal cells has shifted focus towards immunotherapy notable approach. Extracellular vesicles (EVs) naturally occurring transporters, capable delivering tumor-specific antigens directly engaging in immune response. Multiple types EVs have emerged promising platforms for vaccination. The effectiveness EV-based vaccines manifests ability potentiate response, by activating dendritic (DCs) CD8+ T lymphocytes, through engineering synergy antigen presentation targeted delivery. Here, this review mainly focuses on construction strategies EV various sources, effects, immunological mechanisms treating melanoma, well shortcomings future perspectives field. These findings will provide novel insights into innovative exploitation therapy.

Language: Английский

---

Revolutionizing cancer treatment via bioengineered extracellular vesicles: Exploring nanovesicles to fully synthetic solutions

Applied Materials Today, Journal Year: 2024, Volume and Issue: 40, P. 102395 - 102395

Published: Aug. 28, 2024

The prevalence of cancer on a global scale has necessitated the development various therapeutic approaches. However, effectiveness and safety existing methods often face some limitations. Nanotechnology emerged as solution, enabling design nanosystems that can effectively deliver drugs. These address challenges such low drug stability solubility, well lack tumor targetability. While nanomaterials offer advantages, certain nanoparticles have their own limitations, which prompted researchers to explore innovative delivery systems. One promising avenue is use extracellular vesicles (EVs), are natural secreted by cells. EVs possess biocompatibility, stability, targeting capabilities, making them ideal candidates for carriers. still remain in this field. include limited production yield, complexity, inefficient cargo loading, controlled release To overcome these challenges, been employing engineering techniques modify structures EVs, enhancing intrinsic properties. Surface modification hybrid systems combine with other shown potential addressing This review focuses landscape crucial role therapeutics diagnosis. It begins exploring biological functions properties EVs. Additionally, introduces strategies isolating shedding light methodologies used harvest minute entities. Finally, highlights biomedical applications bioengineering treatment.

Language: Английский

---