Published: Jan. 1, 2024
Language: Английский
Agriculture, Journal Year: 2025, Volume and Issue: 15(5), P. 444 - 444
Published: Feb. 20, 2025
This study combines hyperspectral imaging technology with biochemical parameter analysis to facilitate the disease severity evaluation and early detection of lettuce downy mildew. The results reveal a significant negative correlation between index (DI) levels flavonoids (r = −0.523) anthocyanins −0.746), indicating role these secondary metabolites in enhancing plant resistance. Analysis data identified that spectral regions (410–503 nm, 510–615 630–690 nm) vegetation indices like PRI ARI2 were highly correlated DI, flavonoids, anthocyanins, providing potential indicators for assessment detection. Moreover, regression models developed using Partial Least Squares (PLS), Random Forest (RF), Convolutional Neural Network (CNN) algorithms demonstrated high accuracy reliability predicting highest R2 0.857, 0.910, 0.963, respectively. classification model PLS, RF, CNN successfully detected physiological changes within 24 h post-infection (highest 0.764), offering an effective tool key parameters PLS-DA model, PRI, also strong correlations DI. These findings provide scientific basis practical tools managing mildew resistance breeding while laying foundation broader applications pathology.
Language: Английский
Citations
1
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: Feb. 17, 2025
Maize Downy Mildew (MDM) is a devastating disease in the humid sub-tropical/tropical regions of Asia. In this study, prevalence MDM during rainy Kharif seasons south Karnataka state (India) ranged between 6.8% (2018) and 19.1% (2022). The research evaluated new fungicidal treatments assessed genetic tolerance maize lines to develop robust management strategies that enhance productivity stability. During 2021 2022, we conducted field trials evaluate effectiveness six different fungicides, both individually combination. most effective approach combined seed treatment with Metalaxyl (4%) Mancozeb (64%) WP, followed by foliar spray Azoxystrobin (18.2%) Difenoconazole (11.4%) SC. This reduced incidence 97.6% increased yield up 85.6 quintals per hectare, benefit-cost ratio 2.2. Additionally, screening 317 inbred 2019 identified 22 stable resistance over nine consecutive Rabi seasons, indicating their potential for sustained resistance. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis revealed significant increases eighteen phenolic compounds fifteen flavonoid resistant genotypes. Specifically, genotypes exhibited elevated levels salicylic acid (4.2 9.2-fold), p-Coumaric (3.7 4.8-fold), o-Coumaric (4.5 7.4-fold), Caffeic (2.4 3.1-fold), Ferulic (2.3 2.8-fold). Flavonoid also increased, Naringenin ranging from 34.4 µg/g African Tall 130 MAI 224, Catechin 22.9 124.4 10, Epicatechin 1.3 8.2 10. These heightened contribute chemical defence mechanism against Peronosclerospora sorghi. study provides crucial insights into managing through host plant treatments. We as valuable resources breeding MDM-resistant hybrids. Enhanced specific these suggest mechanism, essential developing resilient crops. Our findings offer practical recommendations improving production ensuring crop security MDM-affected regions. Integrating can significantly advance sustainable agricultural practices, contributing resilience food areas prone MDM.
Language: Английский
Citations
0
IOP Conference Series Earth and Environmental Science, Journal Year: 2025, Volume and Issue: 1469(1), P. 012012 - 012012
Published: March 1, 2025
Abstract Downy mildew and leaf blight are the most damaging diseases of maize in subtropical tropical Asia. The presence these two severely limits production. effective ef4icient way to overcome losses due this infectious disease is develop varieties that resistant various pathogenic species. This study aimed identify level resistance accessions against downy so it can be widely used breeding programs. Tests on several were carried out using mass screening techniques inoculation methods for major diseases. Accessions was observed based percentage plant infected. Test results showed there 1 accession (05022-00847) 9 late (05022-00106, 05022-00209, 05022-00765, 05022-00777, 05022-00830, 05022 -00833, 05022-00847, Gumarang, Palakka).
Language: Английский
Citations
0
Discover Artificial Intelligence, Journal Year: 2025, Volume and Issue: 5(1)
Published: May 9, 2025
Language: Английский
Citations
0
Molecular Plant-Microbe Interactions, Journal Year: 2023, Volume and Issue: 36(12), P. 754 - 763
Published: Sept. 26, 2023
Cytoplasmic effectors with an Arg-any amino acid-Arg-Leu (RxLR) motif are encoded by hundreds of genes within the genomes oomycete Phytophthora spp. and downy mildew pathogens. There has been a dramatic increase in our understanding evolution, function, recognition these effectors. Host proteins wide range subcellular localizations functions targeted RxLR Many processes manipulated, including transcription, post-translational modifications, such as phosphorylation ubiquitination, secretion, intracellular trafficking. This involves array effector modes-of-action, stabilization or destabilization protein targets, altering disrupting complexes, inhibition utility target enzyme activities, changing location targets. Interestingly, approximately 50% identified host negative regulators immunity. Avirulence may be directly indirectly detected nucleotide-binding leucine-rich repeat resistance (NLR) proteins. Direct single NLR orthologues conserved across multiple pathogens provide protection diverse crops. Failure to interact appropriately manipulate nonhost plants shown restrict range. knowledge can potentially exploited alter targets prevent interaction, providing barrier infection. Finally, recent evidence suggests that effectors, like cytoplasmic from fungal pathogen Magnaporthe oryzae, enter cells via clathrin-mediated endocytosis. [Formula: see text] Copyright © 2023 The Author(s). is open access article distributed under CC BY-NC-ND 4.0 International license.
Language: Английский
Citations
9
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Sept. 27, 2024
Language: Английский
Citations
3
The Plant Journal, Journal Year: 2023, Volume and Issue: 115(1), P. 108 - 126
Published: March 29, 2023
Lactuca saligna L. is a wild relative of cultivated lettuce (Lactuca sativa L.), with which it partially interfertile. Hybrid progeny suffer from hybrid incompatibility (HI), resulting in reduced fertility and distorted transmission ratios. displays broad-spectrum resistance against downy mildew caused by Bremia lactucae Regel considered non-host species. This phenomenon called (NHR). One possible mechanism behind this NHR through the plant-pathogen interaction triggered pathogen recognition receptors, including nucleotide-binding leucine-rich repeat (NLR) proteins receptor-like kinases (RLKs). We report chromosome-level genome assembly (accession CGN05327), leading to identification two large paracentric inversions (>50 Mb) between sativa. Genome-wide searches delineated major clusters as regions enriched NLRs RLKs. Three co-locate previously identified intervals. RNA-seq analysis Bremia-infected several differentially expressed RLKs regions. tandem wall-associated kinase-encoding genes (WAKs) NHR8 interval display particularly high expression changes at an early stage infection. propose strong candidates for determinants phenotype saligna.
Language: Английский
Citations
7
Sensors, Journal Year: 2024, Volume and Issue: 24(6), P. 1916 - 1916
Published: March 16, 2024
Downy mildew caused by Hyaloperonospora brassicae is a severe disease in Brassica oleracea that significantly reduces crop yield and marketability. This study aims to evaluate different vegetation indices assess downy infection levels the variety Mildis using hyperspectral data. Artificial inoculation H. sporangia suspension was conducted induce of disease. Spectral measurements, spanning 350 nm 1050 nm, were on leaves an environmentally controlled setup, reflectance data acquired processed. The Successive Projections Algorithm (SPA) signal sensitivity calculation used extract most informative wavelengths could be develop (DMI). A total 37 existing three proposed DMIs evaluated indicate (DM) levels. results showed classification support vector machine achieved accuracies 71.3%, 80.7%, 85.3% for distinguishing healthy from DM1 (early infection), DM2 (progressed DM3 (severe infection) index. new index potentially enables development automated DM monitoring system resistance profiling breeding lines.
Language: Английский
Citations
2
New Phytologist, Journal Year: 2024, Volume and Issue: 243(1), P. 330 - 344
Published: May 14, 2024
Arabidopsis Col-0 RPP2A and RPP2B confer recognition of downy mildew (Hyaloperonospora arabidopsidis [Hpa]) isolate Cala2, but the identity recognized ATR2
Language: Английский
Citations
1
Plant Pathology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 4, 2024
Abstract Pseudoperonospora cubensis , causal agent of cucurbit downy mildew, is a common and highly variable pathogen vegetables. Pathogen virulence can be considered on two different levels, that is, pathotypes races. Pathotypes characterize the interactions between isolates set genotypes represented by species and/or genera host plant family. Races are characterized (e.g., cultivars, lines, accessions) one (or very closely related species). Differential sets for pathotype race determination composed distinct (different) genotypes. Until recently, only differentiation was used to P . Most recent research variation in populations Czech Republic working with Cucumis melo differential demonstrated existence large number races this pathogen. The capacity primarily created denomination phenotypes/races powdery mildew ( Golovinomyces orontii Podosphaera xanthii ). However, also efficient differentiating phenotypes This means C independent plant–pathogen systems: mildew. Results showed developing identical reaction patterns at level pathotype, develop vice versa. It explained categories assessment variation, which should clearly defined, recognized within international scientific breeding communities.
Language: Английский
Citations
1