Disciplinarum Scientia. Série Ciências Naturais e Tecnológicas,
Journal Year:
2023,
Volume and Issue:
24(3), P. 57 - 68
Published: Jan. 1, 2023
The
global
population
experienced
an
unprecedented
viral
infection
known
as
SARS-CoV-2.
Due
to
it
being
a
novel
virus,
the
range
of
effective
medications
was
limited.
Nevertheless,
occurrence
undesired
and
often
potentially
toxic
effects
further
restricted
choices
medications.
Considering
this,
nanotechnology,
through
manipulating
matter,
allows
many
compounds
exhibit
nanoscale
properties,
reducing
toxicity,
increasing
selectivity,
lowering
required
dosage.
excellent
properties
nanoparticles
mentioned
in
this
study,
objective
synthesize
magnetic
doped
with
zinc
oxide,
aiming
at
developing
nanomaterial
antiviral
activity.
Once
are
obtained,
subsequent
biological
assays
will
be
conducted
evaluate
their
activity
against
SARS-CoV-2
virus.
Journal of Toxicology and Environmental Health,
Journal Year:
2024,
Volume and Issue:
87(22), P. 879 - 894
Published: Sept. 2, 2024
Melanoma
is
the
most
aggressive
type
of
skin
cancer,
with
few
therapeutic
alternatives
following
metastasis
development.
In
recent
years,
drug
delivery-associated
nanotechnology
has
shown
promising
targeted
results
diminished
adverse
effects
compared
to
conventional
treatments.
This
study
aimed
(1)
examine
plant-derived
α-arbutin,
a
natural
compound
and
(2)
compare
these
findings
bioactively
developed
liposomes
containing
α-arbutin
utilizing
B16-F10
murine
melanoma
cell
line
as
model.
Liposomes
were
obtained
through
reversed-phase
evaporation
by
applying
spray
dryer
assess
their
stability.
The
biologic
assays
measured
cytotoxicity/antiproliferative
(MTT,
Neutral
Red,
dsDNA
PicoGreen).
addition,
levels
melanin
purinergic
enzymes
also
measured.
production
reactive
oxygen
species
(ROS)
nitric
oxide
(NO)
was
determined
measure
oxidative
state.
Treatment
nano-liposome
alpha-arbutin
induced
significant
68.4%
cytotoxicity,
similar
positive
control,
in
at
72
hr.
Further,
arbutin
increased
ROS
nitrite
formation
hr
highest
concentration
(100
300
µg/ml)
Arbutin
reduced
all
tested
concentrations.
lowered
nucleotides
(AMP,
ADP,
ATP)
nucleoside
(adenosine)
cells.
Evidence
suggests
that
liposome
can
be
considered
an
alternative
immunosuppressive
agent
stimulated
treatment.
Journal of Toxicology and Environmental Health,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 16
Published: Jan. 23, 2025
This
study
aimed
to
identify
chemical
compounds
derived
from
Vassobia
breviflora
methanolic
extract
using
ESI-ToF-MS
and
their
antioxidant
potential
activity
utilizing
the
following
methods:
total
phenols,
DPPH,
ABTS•+.
The
MTT
assay
measured
cytotoxic
activity,
while
DCFH-DA
nitric
oxide
assays
were
employed
determine
reactive
oxygen
species
(ROS)
nitrogen
(RNS)
levels
African
green
monkey
kidney
(VERO)
human
keratinocyte
(HaCat)
cell
lines.
minimal
inhibitory
concentration
(MIC)
bactericidal
(MBC)
assessed
in
seven
clinical
isolates
nine
ATCC
strains.
Biofilm
inhibition
was
tested
against
four
biofilm-forming
properties
of
identified
as
follows:
35.74
mg
GAE/g
(gallic
acid
equivalents)/g
for
10.5
µg/ml
50.68
µmol
trolox/µg
mean
(IC50)
values
622.86
784.33
(HaCat).
These
concentrations
did
not
markedly
alter
ROS
RNS.
Conversely,
Bacillus
cereus
β-hemolytic
displayed
higher
sensitivity
extract,
with
MIC
64
MBC
128
µg/ml.
Enterococcus
faecium
exhibited
lowest
biofilm
formation
among
bacteria.
studied
plant
all
bacterial
strains
at
lower
than
IC50
VERO
HaCat
cells,
suggesting
future
studies.
Data
present
a
comprehensive
molecular
docking
analysis
HlyIIR
protein
(PDB
ID:
2FX0)
determined
antimicrobial
endocrine-modulating
potentials.
Notably,
lancifodilactone
I
nicandrin
B
demonstrated
strongest
binding
affinities,
energies
-9.8
kcal/mol
-8.3
kcal/mol,
respectively,
significant
effects
B.
cereus.
In
addition,
several
showed
interactions
nuclear
receptors,
indicating
effects.
findings
provide
insights
into
developing
target-specific
therapies
agents.
