Molecular Detection and Phylogenetic Analysis of Deformed Wing Virus and Sacbrood Virus Isolated from Pollen DOI Creative Commons
Ralitsa Balkanska, Rositsa Shumkova, Nedyalka Atsenova

et al.

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(2), P. 140 - 140

Published: Feb. 10, 2023

Among many pathogens and pests, honey bee viruses are known as one of the most common cause diseases in colonies. In this study, we demonstrate that pollen grains bread potential sources viral DNA. We extracted DNA from 3 types samples: directly provided by beekeepers (n = 12), purchased trade markets 5), obtained honeycombs (bee bread, n 10). The was used for molecular detection (RT-PCR analysis) six widely distributed viruses: deformed wing virus, sacbrood acute paralysis black queen cell Kashmir Israeli chronic virus. successfully managed to establish only virus (DWV) (SBV), with different distribution frequencies depending on territory country. phylogenetic analyses Bulgarian isolates were performed similar sequences available databases other countries. Phylogenies strains demonstrated genetically heterogeneous populations DWV relatively homogenous SBV. conclusion, results current study have shown is a valuable source pathogens. This allows epidemiological monitoring at regional national level.

Language: Английский

Prospects of probiotics in beekeeping: a review for sustainable approach to boost honeybee health DOI

Smriti,

Anita Rana,

Gagandeep Singh

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 4, 2024

Language: Английский

Citations

12
Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey – A review DOI Creative Commons

Liliana Luca,

Daniela Pauliuc, Mircea Oroian

et al.

Food Chemistry X, Journal Year: 2024, Volume and Issue: 23, P. 101524 - 101524

Published: June 5, 2024

Honey is a natural product used since ancient times due to its taste, aroma, and therapeutic properties (antibacterial, antiviral, anti-inflammatory, antioxidant activity). The purpose of this review present the species microorganisms that can survive in honey effect they have on bees consumers. techniques for identifying are also described study. contains bacteria, yeasts, molds, viruses, some them may beneficial humans. antimicrobial acidity high viscosity, sugar concentration, low water content, presence hydrogen peroxide non-peroxidase components, particularly methylglyoxal (MGO), phenolic acids, flavonoids, proteins, peptides, glycopeptides. has antibacterial action (it effectiveness against e.g. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter, etc.), antifungal (effectiveness Candida spp., Aspergillus Fusarium Rhizopus Penicillium spp.), antiviral SARS-CoV-2, Herpes simplex virus type 1, Influenza A B, Varicella zoster virus), antiparasitic Plasmodium berghei, Giardia Trichomonas, Toxoplasma gondii) demonstrated by numerous studies comprised discussed review.

Language: Английский

Citations

11
Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees? DOI Creative Commons
Chiara Braglia, Daniele Alberoni,

Paula Melisa Garrido

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 13, 2024

The genus Serratia harbors opportunistic pathogenic species, among which marcescens is for honeybees although little studied. Recently, virulent strains of S. colonizing the Varroa destructor mite’s mouth were found vectored into honeybee body, leading to septicemia and death. also occurs as an pathogen in honeybee’s gut with a low absolute abundance. population seems controlled by host immune system, but its presence may represent hidden threat, ready arise when are weakened biotic abiotic stressors. To shed light on pathogen, this research aims at studying ’s development dynamics body interactions co-occurring fungal Vairimorpha ceranae . Firstly, degree pathogenicity ability permeate epithelial barrier three strains, isolated from belonging different species ( , liquefaciens nematodiphila ), assessed artificial inoculation newborn doses (10 4 10 6 8 cells/mL). abundance hemocoel was qPCR primers targeting luxS gene. Moreover, infected V. stages supplied beneficial microorganisms fumagillin. Our results showed that all tested could pass through proliferate hemocoel, being most pathogenic. under cage conditions, better proliferates infection co-occurring, positive significant correlation. Finally, fumagillin some control both development. findings suggest correlation between two pathogens laboratory should be taken consideration researches testing antimicrobial compounds active against related survival rate. our environmental microorganism Apilactobacillus kunkeei vivo model, confirming potential specie bacteria honeybees.

Language: Английский

Citations

10
Micro”bee”ota: Honey Bee Normal Microbiota as a Part of Superorganism DOI Creative Commons
Daniil Smutin, Egor Lebedev,

Maxim Selitskiy

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(12), P. 2359 - 2359

Published: Nov. 29, 2022

Honey bees are model organisms for microbiota research. Gut microbiomes very interesting surveys due to their simple structure and relationship with hive production. Long-term studies reveal the gut patterns of various members, as well functions, sources, interactions majority its bacteria. But fungal non-pathogenic part is almost unexplored, likewise some other related microbiota. bees, superorganisms, interact own microorganisms, microbial communities food stores, surfaces, environments. Understanding diversity, transition ways, niche colonization control necessary understanding any separate because interplay. The long coevolution microorganisms populating these niches makes systems co-dependent, integrated, stable. Interaction environment, hive, determines caste lifestyle individual In this article, we bring together on western honey bee. We show a possible between determination composition. And what primary: differentiation or composition?

Language: Английский

Citations

32
Prevalence of honey bee pathogens and parasites in South Korea: A five-year surveillance study from 2017 to 2021 DOI Creative Commons
A‐Tai Truong, Mi-Sun Yoo,

Soo Kyoung Seo

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(2), P. e13494 - e13494

Published: Feb. 1, 2023

Honey bees play an important role in the pollination of crops and wild plants provide products to humans. Pathogens parasites are main factors that threaten beekeeping South Korea. Therefore, a nationwide detection 14 honey bee pathogens, including (phorid flies, Nosema ceranae, Acarapis woodi mites), viruses, bacteria, fungal was conducted from 2017 2021 country. The infection rate trend each pathogenic agent were determined. A total 830 samples Apis cerana (n = 357) A. mellifera 473) examined. N. ceranae (35.53%), deformed wing virus (52.63%), sacbrood (SBV) black queen cell (55.26%) most prevalent their prevalence rapidly increased 2021. Paenibacillus larvae, Israeli acute paralysis virus, Ascosphaera apis, woodi, Melissococcus plutonius, chronic remained stable during surveillance period, with rates ranging 5.26% 16.45% Other phorid Kashmir Aspergillus flavus, had low gradually declined period. occurrence honeybee pathogens peaked July. SBV common pathogen cerana, whereas predominant mellifera. This study provides information regarding current status presents These data for predicting outbreaks diseases

Language: Английский

Citations

18
Global honeybee health decline factors and potential conservation techniques DOI

Yinying Yang,

Yuzheng Wu,

Hexuan Long

et al.

Food Security, Journal Year: 2023, Volume and Issue: 15(4), P. 855 - 875

Published: Jan. 18, 2023

Language: Английский

Citations

17
Biotic and abiotic stresses on honeybee health DOI Creative Commons
Zheguang Lin,

Siyi Shen,

Kang Wang

et al.

Integrative Zoology, Journal Year: 2023, Volume and Issue: 19(3), P. 442 - 457

Published: July 10, 2023

Honeybees are the most critical pollinators providing key ecosystem services that underpin crop production and sustainable agriculture. Amidst a backdrop of rapid global change, this eusocial insect encounters succession stressors during nesting, foraging, pollination. Ectoparasitic mites, together with vectored viruses, have been recognized as central biotic threats to honeybee health, while spread invasive giant hornets small hive beetles also increasingly threatens colonies worldwide. Cocktails agrochemicals, including acaricides used for mite treatment, other pollutants environment widely documented affect bee health in various ways. Additionally, expanding urbanization, climate agricultural intensification often result destruction or fragmentation flower-rich habitats. The anthropogenic pressures exerted by beekeeping management practices natural selection evolution honeybees, colony translocations facilitate alien species invasion disease transmission. In review, multiple abiotic their interactions potentially undermine discussed, taking into consideration sensitivity, large foraging area, dense network among related nestmates, social behaviors honeybees.

Language: Английский

Citations

15
Analysis of Elements and Physicochemical and Microbial Properties of Iranian Honeys DOI
Majid Arabameri,

Mahsa Naghashan,

Mahsa Ahmadloo

et al.

Biological Trace Element Research, Journal Year: 2023, Volume and Issue: 202(9), P. 4279 - 4287

Published: Dec. 14, 2023

Language: Английский

Citations

14
Comparative effect of probiotic and antibiotic on honey bees colony functional traits DOI Creative Commons
Maghsoud Besharati,

Reza Bavand,

Hamid Paya

et al.

The EuroBiotech Journal, Journal Year: 2024, Volume and Issue: 8(1), P. 1 - 11

Published: Jan. 1, 2024

Abstract The purpose of this study was to evaluate the effect different levels probiotic on colony functional traits, honey quantitative and qualitative parameters gut microbial flora in bees. A total 56 hives were randomly allocated 8 treatments with 7 replicates city Firouzkoh (Iran) for about 60 days. Experimental that included: control, 2 g antibiotic, 1 probiotic, 3 antibiotic + all per liter syrup each hive. results showed fed or had higher hive population production than other (p<0.05). Adding increased lactobacillus counts bees’ intestines compared Also, supplementation decreased Escherichia coli intestine adding fructose glucose sucrose present suggest might be used as a feed additive quality improvement

Language: Английский

Citations

5
BHiveSense: An integrated information system architecture for sustainable remote monitoring and management of apiaries based on IoT and microservices DOI Creative Commons

Duarte Cota,

José Martins, Henrique São Mamede

et al.

Journal of Open Innovation Technology Market and Complexity, Journal Year: 2023, Volume and Issue: 9(3), P. 100110 - 100110

Published: Aug. 4, 2023

Precision Beekeeping, a field of Agriculture, is an apiary management strategy based on monitoring honeybee colonies to promote more sustainable resource usage and maximise productivity. The approach related Beekeeping methodologies mitigate the stress associated with human intervention in waste resources. These goals are achieved by supporting managing beekeeper's timely appropriate action at colony's level. In recent years, growth IoT (Internet-of-Things) Agriculture has spurred several proposals contribute paradigm built different technical concepts production costs. This work proposes describes information systems architecture concept named BHiveSense, microservices, artefacts demonstrate its concept: (1) low-cost COTS (Commercial Off-The-Shelf) hive sensing prototype, (2) REST backend API, (3) Web application, (4) Mobile application. project delivers solution for integrated beekeeping activity. Our stresses that adopting microservices architecture, it possible deal long-standing problems concerning interoperability, scalability, agility, maintenance issues, delivering efficient beehive system.

Language: Английский

Citations

13