Design and Application of Fluorescent Probes to Detect Cellular Physical Microenvironments

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(4), P. 1738 - 1861

Published: Feb. 14, 2024

The microenvironment is indispensable for functionality of various biomacromolecules, subcellular compartments, living cells, and organisms. In particular, physical properties within the biological could exert profound effects on both cellular physiology pathology, with parameters including polarity, viscosity, pH, other relevant factors. There a significant demand to directly visualize quantitatively measure fluctuation in spatiotemporal resolution. To satisfy this need, analytical methods based fluorescence probes offer great opportunities due facile, sensitive, dynamic detection that these molecules enable varying settings from vitro samples live animal models. Herein, we focus types small molecule fluorescent measurement microenvironment, mechanical force, temperature, electron potential. For each parameter, primarily describe chemical mechanisms underlying how are correlated changes signals. This review provides an overview perspective development environment, expand knowledge process, enrich diagnostic tools human diseases.

Language: Английский

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A hemicyanine-based fluorescent probe for ratiometric detection of ClO- and turn-on detection of viscosity and its imaging application in mitochondria of living cells and zebrafish

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 383, P. 133510 - 133510

Published: Feb. 13, 2023

A water-soluble fluorescent probe 1 based on chromene-hemicyanine has been developed, that exhibits ratiometric response toward hypochlorite (ClO-) with the LOD (0.082 μM), and "turn-on" to viscosity changes. Due low cytotoxicity good biocompatibility, used monitor hypochlorous acid in mitochondria of living cells zebrafish.

Language: Английский

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A review: Small organic molecule dual/multi-organelle-targeted fluorescent probes

Talanta, Journal Year: 2023, Volume and Issue: 259, P. 124529 - 124529

Published: April 7, 2023

Language: Английский

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Dissecting lysosomal viscosity fluctuations in live cells and liver tissues with an ingenious NIR fluorescent probe

Talanta, Journal Year: 2024, Volume and Issue: 272, P. 125825 - 125825

Published: Feb. 23, 2024

Language: Английский

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Near-Infrared Probes Designed with Hemicyanine Fluorophores Featuring Rhodamine and 1,8-Naphthalic Derivatives for Viscosity and HSA Detection in Live Cells

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

This paper presents the development of near-infrared (NIR) fluorescent probes,

Language: Английский

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Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Chemistry - A European Journal, Journal Year: 2020, Volume and Issue: 27(23), P. 6880 - 6898

Published: Dec. 19, 2020

Abstract Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more interest. However, owing the complicated character, it still huge challenge directly observe in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated map inside live cells small animals. minireview, viscosity‐sensitive molecular fluorescent used bioimaging comprehensively summarized, mainly focusing on last three years. Moreover, current challenges opportunities for development viscosity‐specific will be discussed.

Language: Английский

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A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

Chemical Communications, Journal Year: 2021, Volume and Issue: 57(36), P. 4376 - 4379

Published: Jan. 1, 2021

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which applied detect the intracellular and imagine pancreatic tissue in diabetic mouse model.

Language: Английский

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Novel meso-trifluoromethyl BODIPY-based near-infrared-emitting fluorescent probes for organelle-specific imaging of cellular viscosity

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 359, P. 131594 - 131594

Published: Feb. 18, 2022

Language: Английский

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Dihydroxanthene-Based Near-infrared Fluorescent Probes for Monitoring Mitochondrial Viscosity in Living Cells and Mice

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3544 - 3549

Published: Feb. 6, 2023

Aberrant mitochondrial viscosity is closely associated with many diseases and cellular malfunctions. Thus, the development of reliable methods for monitoring variations has attracted considerable attention. Herein, through stepwise structural modulation dihydroxanthene fluorophore (DHX), we developed three NIR fluorescent probes, named DHX-V-1-3, detecting viscosity. Among them, DHX-V-3 displayed highest signal-to-noise ratio (67-fold) outstanding selectivity showed excellent mitochondria targeting immobilization ability. At level, probe was successfully applied to image in live cells upon treatment lipopolysaccharide (LPS) or nystatin. Moreover, benefiting from its emission increased depth tissue imaging, demonstrated ability visualize LPS-treated mice.

Language: Английский

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A single fluorescent probe to examine the dynamics of mitochondria-lysosome interplay and extracellular vesicle role in ferroptosis

Developmental Cell, Journal Year: 2024, Volume and Issue: 59(4), P. 517 - 528.e3

Published: Jan. 24, 2024

Language: Английский

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