State-of-the-art cancer biomarker detection by portable (Bio) sensing technology: A critical review

Microchemical Journal, Journal Year: 2022, Volume and Issue: 177, P. 107248 - 107248

Published: Feb. 9, 2022

Language: Английский

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Aptamer-based rapid diagnosis for point-of-care application

Microfluidics and Nanofluidics, Journal Year: 2023, Volume and Issue: 27(2)

Published: Jan. 18, 2023

Language: Английский

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Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 252, P. 116142 - 116142

Published: Feb. 20, 2024

Nanostructured electrochemical biosensors have ushered in a new era of diagnostic precision, offering enhanced sensitivity and specificity for clinical biomarker detection. Among them, capacitive biosensing enables ultrasensitive label-free detection multiple molecular targets. However, the complexity cost associated with conventional fabrication methods nanostructured platforms hinder widespread adoption these devices. This study introduces biosensor that leverages laser-engraved reduced graphene oxide (rGO) electrodes decorated gold nanoparticles (AuNPs). The involves laser-scribed GO-Au

Language: Английский

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Interleukin-6 electrochemical sensor using poly(o-phenylenediamine)-based molecularly imprinted polymer

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 404, P. 135282 - 135282

Published: Jan. 5, 2024

Interleukin-6 (IL-6) is an important cytokine involved in immune responses and maintaining body homeostasis. Elevated IL-6 levels, exceeding ~40 pg/mL bodily fluids, are associated with inflammation diseases such as COVID-19, cardiovascular disorders, Alzheimer's, necessitating real-time health monitoring for personalized healthcare. In this study, we developed a highly sensitive selective sensing platform by depositing poly(o-phenylenediamine) (P(o-PD))-based molecularly imprinted polymer (MIP) onto oxygen-functionalized screen-printed carbon electrode gold nanoparticles, 3-aminopropyltriethoxysilane (APTES), glutaraldehyde (GA). While APTES enhanced the peak current due to redox probe adsorption, GA reduced it non-conductive aldehyde groups. This functionalized surface improved hydrophilicity presence of amino carbonyl We identified that 120-minute NaCl treatment effectively removed templates, ensuring successful embedding P(o-PD) matrix. calculated imprinting factor 11.2, indicating effective specific binding sites within MIP, resulting robust response IL-6. After optimizing MIP deposition through five cycles, detected concentrations ranging from 2 400 pg/mL, sensitivity 3.48 μA/log(pg/mL) limit detection 1.74 pg/mL. When tested real human serum, sensor encountered challenges matrix adsorbing other active molecules. Despite challenge, our demonstrates high selectivity long-term stability detection. These qualities position well-suited point-of-care diagnostics, emphasizing its potential reliable tool real-world applications.

Language: Английский

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A dual-purpose electrode material for voltametric quantification of uric acid and supercapacitor performance using dysprosium-doped CaZrO3

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113081 - 113081

Published: Feb. 1, 2025

Language: Английский

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Recent Advances of Point-of-Care Devices Integrated with Molecularly Imprinted Polymers-Based Biosensors: From Biomolecule Sensing Design to Intraoral Fluid Testing

Biosensors, Journal Year: 2022, Volume and Issue: 12(3), P. 136 - 136

Published: Feb. 22, 2022

Recent developments of point-of-care testing (POCT) and in vitro diagnostic medical devices have provided analytical capabilities reliable results for rapid access at or near the patient’s location. Nevertheless, challenges diagnosis still remain an important factor actual clinical trials before on-site treatment making decisions. New classes POCT depict precise technologies that can detect biomarkers biofluids such as sweat, tears, saliva urine. The introduction a novel molecularly imprinted polymer (MIP) system artificial bioreceptor could be one emerging candidates to improve performance along with physicochemical stability when used harsh environments. Here, we review potential availability MIP-based biorecognition systems custom receptors high selectivity chemical affinity specific molecules. Further progress advanced MIP technology biomolecule recognition are introduced. Finally, POCT-based system, summarized perspectives expandability periodontal future directions biosensors wearable format.

Language: Английский

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Using Graphene-Based Biosensors to Detect Dopamine for Efficient Parkinson’s Disease Diagnostics

Biosensors, Journal Year: 2021, Volume and Issue: 11(11), P. 433 - 433

Published: Oct. 31, 2021

Parkinson’s disease (PD) is a neurodegenerative in which the neurotransmitter dopamine (DA) depletes due to progressive loss of nigrostriatal neurons. Therefore, DA measurement might be useful diagnostic tool for targeting early stages PD, as well helping optimize replacement therapy. Moreover, sensing appears analytical complex biological systems PD studies. To support feasibility this concept, mini-review explores currently developed graphene-based biosensors dedicated detection. We discuss various graphene modifications designed high-performance electrodes alongside their performances and interference studies, we listed based on limit detection samples. optical are also presented herein. Regarding clinical relevance, explored development trends electrochemical they relate point-of-care testing suitable site-of-location diagnostics needed personalized management. In field, into smartphone-connected intelligent However, highlighted that focus should utility rather than technical performance.

Language: Английский

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Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

Analytical and Bioanalytical Chemistry, Journal Year: 2022, Volume and Issue: 415(6), P. 1033 - 1063

Published: Sept. 14, 2022

Language: Английский

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Electrochemical Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein in Nasopharyngeal Samples

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(11), P. 4712 - 4719

Published: March 9, 2022

Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable quantitatively presence virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting nucleocapsid protein (N protein). Samples are added to electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection also N protein. The concentration is quantified chronoamperometry 3,3'5,5'-tetramethylbenzidine. Limits equivalent less than 50 plaque forming units/mL (PFU/mL) were determined sample volumes 20 μL. No cross-reactivity was detected influenza and other coronavirus proteins. Patient tested as part proof-of-concept clinical study where gold-standard real-time quantitative polymerase chain reaction (RT-qPCR) method. Preliminary results from data set 22 demonstrated specificity 100% (n = 9 negative according RT-qPCR) sensitivity 70% RT-PCR cycle threshold (Ct) values under 30 10) Ct 25 5), which complies World Health Organization (WHO) criteria POC COVID-19 diagnostic tests. Our SPCEs validated against standard assays, very good agreement found between both (R2 0.9993, n 6), suggesting that our assay could be used assess infectivity. takes 70 min sampling results.

Language: Английский

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A Review on Potential Electrochemical Point-of-Care Tests Targeting Pandemic Infectious Disease Detection: COVID-19 as a Reference

Chemosensors, Journal Year: 2022, Volume and Issue: 10(7), P. 269 - 269

Published: July 11, 2022

Fast and accurate point-of-care testing (POCT) of infectious diseases is crucial for diminishing the pandemic miseries. To fight coronavirus disease 2019 (COVID-19), numerous interesting electrochemical (POC) tests have been evolved to rapidly identify causal organism SARS-CoV-2 virus, its nucleic acid antigens, antibodies patients. Many those biosensors are impressive in terms miniaturization, mass production, ease use, speed test, they could be recommended future applications pandemic-like circumstances. On other hand, self-diagnosis, sensitivity, specificity, surface chemistry, components, device configuration, portability, small analyzers, features can yet improved. Therefore, this report reviews developmental trend POC (i.e., test platforms features) reported rapid diagnosis COVID-19 correlates any significant advancements with relevant references. POCTs incorporating microfluidic/plastic chips, paper devices, nanomaterial-aided platforms, smartphone integration, epidemiological reporting attributes also surfed help preparedness. This review especially screens low-cost easily affordable setups so that management becomes faster easier. Overall, a wide-ranging package finding appropriate strategies POCT targeting detection.

Language: Английский

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