Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria DOI Creative Commons

Meynard R. Austria,

Jon Patrick T. Garcia, Alvin R. Caparanga

et al.

Computation, Journal Year: 2023, Volume and Issue: 11(10), P. 190 - 190

Published: Sept. 30, 2023

Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for detection malaria. However, collection and transportation samples processing dissemination results via conventional PCR, especially when used routine clinical practice, can hamper technique’s sensitivity specificity. The rampancy such disease Philippines aggravated by limited supply medical machinery poor economic state country; thus, need to innovate a device early malaria necessary. With that, this study focuses on designing microfluidic that will mimic function genus-specific PCR based 18S rRNA gene detect parasites (Plasmodium falciparum) at low-grade parasitemia. design was intended be portable, accessible, economical, which none from past literature has dealt with specifically detection. This silico first country specially crafted reasons. proposed developed simulated using ANSYS software Computational Fluid Dynamics (CFD) analyses. simulation shows adding loops increases its relative deviation but minimally compared having only straight path design. indicates looping acceptable minimize chip length. It also found increasing cross-sectional area fluid decreases efficiency Lastly, among three materials utilized, made polypropylene most efficient, 0.94 polycarbonate polydimethylsiloxane, have deviations 2.78 1.92, respectively. Future researchers may mesh 44-cycle due limitations study, other materials, as biocomposites, assessed broaden application

Language: Английский

Microfluidics: a concise review of the history, principles, design, applications, and future outlook DOI
Mohammad Irfan Hajam, Mohammad Mohsin Khan

Biomaterials Science, Journal Year: 2023, Volume and Issue: 12(2), P. 218 - 251

Published: Nov. 27, 2023

This review offers a reliable platform for comprehending microfluidics, covering key concepts, historical advancements, technological evolution, materials, successful implementations, applications, market trends, and future prospects.

Language: Английский

Citations

29
Zweifach–Fung Microfluidic Device for Efficient Microparticle Separation: Cost-Effective Fabrication Using CO2 Laser-Ablated PMMA DOI Creative Commons
Cristian F. Rodríguez,

Mateo Báez-Suárez,

Carolina Muñoz-Camargo

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(7), P. 932 - 932

Published: July 22, 2024

Microfluidic separators play a pivotal role in the biomedical and chemical industries by enabling precise fluid manipulations. Traditional fabrication of these devices typically requires costly cleanroom facilities, which limits their broader application. This study introduces novel microfluidic device that leverages passive Zweifach–Fung principle to overcome financial barriers. Through Lagrangian computational simulations, we optimized an eleven-channel configuration achieved perfect 100% recall rate for particles following specified normal distribution. Experimental evaluations determined 2 mL/h as optimal total flow (TFR), under showcased exceptional performance enhancements precision micrometer-sized particles, achieving overall accuracy 94% ± 3%. Fabricated using cost-effective, non-cleanroom method, this approach represents significant shift from conventional practices, dramatically reducing production costs while maintaining high operational efficacy. The cost each chip is less than USD 0.90 cents manufacturing process takes only 15 min. development not makes technology more accessible but also sets new standard future advancements field.

Language: Английский

Citations

9
High-throughput identification of meat ingredients in adulterated foods based on centrifugal integrated purification-CRISPR array DOI
Xinran Xiang,

Jiaran Lu,

Mengying Tao

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 443, P. 138507 - 138507

Published: Jan. 21, 2024

Language: Английский

Citations

6
Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein DOI Creative Commons
Szu-Jui Chen, Song-Yu Lu, Chin‐Chung Tseng

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(6), P. 283 - 283

Published: May 30, 2024

A microfluidic immuno-biosensor detection system consisting of a spectrum chip and micro-spectrometer device is presented for the rapid point-of-care (POC) quantification high-sensitivity C-reactive protein (hs-CRP) in urine. The process utilizes highly specific enzyme-linked immunosorbent assay (ELISA) method, which capture antibodies are pre-deposited on substrate microchip used to form an immune complex with target antigen. Horseradish peroxidase (HRP) added as marker enzyme, followed by colorimetric reaction using 3,3',5,5'-tetramethylbenzidine (TMB). absorbance values (a.u.) compounds measured measure corresponding hs-CRP concentration according pre-established calibration curve. It shown that can be determined within 50 min. In addition, achieves recovery rates 93.8-106.2% blind water samples 94.5-104.6% artificial results showed CRP 41 urine from patients chronic kidney disease (CKD) were consistent conventional homogeneous particle-enhanced turbidimetric immunoassay (PETIA) method's (R

Language: Английский

Citations

4
Investigation on the linear cooling method of microfluidic chip based on thermoelectric cooler DOI
Dongfang Sun, Han Xue, Haoqing Wang

et al.

Energy, Journal Year: 2024, Volume and Issue: 308, P. 132933 - 132933

Published: Aug. 23, 2024

Language: Английский

Citations

4
Handheld microfluidic multiple detection device for concurrent blood urea nitrogen and creatinine ratio determination using colorimetric approach DOI
C. M. Ko, Chin‐Chung Tseng, Song-Yu Lu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136585 - 136585

Published: Sept. 7, 2024

Language: Английский

Citations

4
Rapid Microfluidic Ion-Exchange Optode System for Point-of-Care Determination of Sodium Concentration in Serum DOI Creative Commons
Kuan-Hsun Huang, Cheng-Xue Yu, Chia-Chun Lee

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 104 - 104

Published: Feb. 12, 2025

A microfluidic system for detecting sodium ions (Na+) has been developed, incorporating a micro finger-pump chip and micro-spectrometer platform to measure Na+ concentration in human serum. small volume (10 μL) of serum sample is introduced into the microchip reacted with preloaded reagent mixture through two-step actuation process. The resulting purple complex directed detection area analyzed using at wavelengths 555 666 nm. then inversely derived from measured A555/A666 absorbance ratio self-written software installed on Raspberry Pi. entire process completed just 3 min, offering significant advantage meeting clinical needs compared traditional reporting turnaround time several hours medical institutions. experimental results indicate linear relationship between within range 1-200 mM, correlation coefficient R2 = 0.9989. Additionally, 60 samples collected chronic kidney disease (CKD) patients showed strong agreement those obtained conventional indirect ion-selective electrode (ISE) method, achieving 0.9885 an average recovery rate 99.4%. In summary, proposed provides practical, affordable, rapid alternative methods, making it highly promising point-of-care (POC) testing applications.

Language: Английский

Citations

0
Design and Optimization of Ultrasound-Assisted Nucleic Acid Extraction System DOI
Y F Huang, Jiayu Yang,

Juntian Zeng

et al.

IEEE Transactions on Instrumentation and Measurement, Journal Year: 2024, Volume and Issue: 73, P. 1 - 14

Published: Jan. 1, 2024

Nucleic acid extraction is the first step in nucleic analysis. Currently, designing an efficient, robust, and automated system has been a challenging topic. Moreover, commonly designed based on magnetic beads method which most widely used clinical practice, yet there lack of methods to characterize measure fluid motion processes. This leads researchers rely biological experimental results for evaluating performance system. To address these issues, we proposed highly efficient system, including ultrasound-assisted instrument microfluidic chip. integrated ultrasound technology throughout process, significantly enhanced efficiency while eliminating need complex functional modules or additional reagent materials. It can complete within 12 minutes, whereas manual other instruments typically take 30 minutes more. Furthermore, presented non-contact flow pattern measurement approach that combines acoustic knowledge machine learning, be capture miniature confined chamber under ultrasonic stimulation. Multidimensional experiments were conducted Firstly, annotated 1946 field images achieved average recognition precision 96.4%. Based measurements analysis actual fields, enhancements made stimulation strategy, improved performance. As final point, verification indicated performed comparably "gold standard" method. The Coefficient Variation (CV) remained 5%, signaling excellent stability. Particularly, it demonstrated 100% detection rate low-concentration samples (1.7 × 10 3 copies/mL).

Language: Английский

Citations

3
Mobile lab: A novel pathogen assay using the nucleic acid automatic assay system assisted by a self-contained microfluidic cassette and chitosan decorating magnetic particles DOI
Ke Yang,

Jiujian Zhou,

Jun Zhao

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 419, P. 136413 - 136413

Published: Aug. 3, 2024

Language: Английский

Citations

2
Simultaneous Detection of Biomarkers in Urine Using a Multicalibration Potentiometric Sensing Array Combined with a Portable Analyzer DOI

Shengqi Cheng,

Fan Wang, Shaohua Zuo

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 17, 2024

Domestic monitoring devices make real-time and long-term health possible, allowing people to track their status regularly. Uric acid (UA), creatinine, urea in urine are three important biomarkers for various diseases, especially kidney diseases. This work proposed a 10-channel potentiometric sensing array containing UA electrode group, creatinine pH one pair of reference channels, which could be connected with portable analyzer, realizing the simultaneous detection UA, urea, urine. The prepared Pt/carbon nanotubes (CNTs)-uricase, deiminase, Au@urease, polyaniline were employed as materials, showing responses four targets high sensitivity selectivity. To improve accuracy domestic monitoring, calibration channel was integrated into each group calibrate basic potential influences temperature on investigated through an external probe slope intercept. With preset deduced parameters computational formula analyzer Lab Mode, concentrations human samples directly displayed screen Practical Mode. agreement these results those obtained from commercial kits meters reveals methods developing facilitate monitoring.

Language: Английский

Citations

2