Production and purification of outer membrane vesicles encapsulating green fluorescent protein from Escherichia coli: a step towards scalable OMV technologies DOI Creative Commons
Julian Daniel Torres-Vanegas,

Nicolas Rincon-Tellez,

Paula Guzmán-Sastoque

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 14, 2024

Outer membrane vesicles (OMVs) are spherical structures that contain a small fraction of the periplasm Gram-negative bacteria, surrounded by its outer membrane. They naturally produced and detached from bacterial surface, participate in diverse biological processes, their diameter size is range 10–300 nm. OMVs have gained interest different applications, such as development biosensors, vaccines, protein chips, encapsulation heterologous proteins peptides expressed these microorganisms. However, use applications limited due to low yields high purification costs. In this study, we green fluorescent (GFP) encapsulated into using Escherichia coli JC8031 transformed with pTRC99A-ssTorA-GFP establish production route. Results showed motility strain prevents immobilization alginate, which hampers OMVs. To address issue, zeolite-based column was used chromatographically separate smaller particles. Further experiments will be focused on standardizing at scalable level.

Language: Английский

Classical and emerging approximations for the screening of antimicrobial peptide libraries DOI
Cristian F. Rodríguez, Valentina Quezada,

Valentina Andrade-Pérez

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 195 - 232

Published: Jan. 1, 2025

Language: Английский

Citations

1
Assessment of CRISPRa-mediated gdnf overexpression in an In vitro Parkinson’s disease model DOI Creative Commons
Paula Guzmán-Sastoque,

Sebastián Sotelo,

Natalia P. Esmeral

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 8, 2024

Introduction Parkinson’s disease (PD) presents a significant challenge in medical science, as current treatments are limited to symptom management and often carry side effects. Our study introduces an innovative approach evaluate the effects of gdnf overexpression mediated by CRISPRa vitro model disease. The expression can have neuroprotective effects, being related modulation neuroinflammation pathways associated with cell survival, differentiation, growth. Methods We developed targeted delivery system using magnetite nanostructured vehicle for efficient transport genetic material. This has resulted substantial increase, up 200-fold) In mixed primary culture astrocytes, neurons, microglia. Results Discussion exhibits endosomal escape more than 56%, crucial effective activation material within cells. increased correlates notable reduction MAO-B complex activity, reaching basal values 14.8 μU/μg protein, reactive oxygen species. Additionally, there is 34.6% increase viability treated neurotoxin MPTP. shows that increasing remediate some cellular symptoms novel system.

Language: Английский

Citations

5
Recent Advances in Polymer Science and Fabrication Processes for Enhanced Microfluidic Applications: An Overview DOI Creative Commons
María Alexandre-Franco, Rahmani Kouider, Raúl Kassir Al-Karany

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(9), P. 1137 - 1137

Published: Sept. 6, 2024

This review explores significant advancements in polymer science and fabrication processes that have enhanced the performance broadened application scope of microfluidic devices. Microfluidics, essential biotechnology, medicine, chemical engineering, relies on precise fluid manipulation micrometer-sized channels. Recent innovations materials, such as flexible, biocompatible, structurally robust polymers, been pivotal developing advanced systems. Techniques like replica molding, microcontact printing, solvent-assisted injection 3D printing are examined, highlighting their advantages recent developments. Additionally, discusses diverse applications polymer-based devices biomedical diagnostics, drug delivery, organ-on-chip models, environmental monitoring, industrial processes. paper also addresses future challenges, including enhancing resistance, achieving multifunctionality, ensuring biocompatibility, scaling up production. By overcoming these potential for widespread adoption impactful use technologies can be realized.

Language: Английский

Citations

5
Enhancing Magnetic Micro- and Nanoparticle Separation with a Cost-Effective Microfluidic Device Fabricated by Laser Ablation of PMMA DOI Creative Commons
Cristian F. Rodríguez, Paula Guzmán-Sastoque,

Carolina Muñoz-Camargo

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(8), P. 1057 - 1057

Published: Aug. 22, 2024

Superparamagnetic iron oxide micro- and nanoparticles have significant applications in biomedical chemical engineering. This study presents the development evaluation of a novel low-cost microfluidic device for purification hyperconcentration these magnetic particles. The device, fabricated using laser ablation polymethyl methacrylate (PMMA), leverages precise control over fluid dynamics to efficiently separate particles from non-magnetic ones. We assessed device’s performance through Multiphysics simulations empirical tests, focusing on separation magnetite blue carbon dots microparticles polystyrene at various total flow rates (TFRs). For nanoparticle separation, achieved recall up 93.3 ± 4% precision 95.9 1.2% an optimal TFR 2 mL/h, significantly outperforming previous models, which only 50% recall. Microparticle demonstrated accuracy 98.1 1% mL/h both experimental conditions. Lagrangian model effectively captured microparticle microparticles, with close agreement between simulated results. Our findings underscore robust capability distinguishing nanoscales. highlights potential low-cost, non-cleanroom manufacturing techniques produce high-performance devices, thereby expanding their accessibility applicability industrial research settings. integration continuous magnet, as opposed segmented magnets designs, was identified key factor enhancing efficiency.

Language: Английский

Citations

4
Nanotheranostics Revolutionizing Gene Therapy: Emerging Applications in Gene Delivery Enhancement DOI Creative Commons
Paula Guzmán-Sastoque, Cristian F. Rodríguez,

María Camila Monsalve

et al.

Journal of Nanotheranostics, Journal Year: 2025, Volume and Issue: 6(2), P. 10 - 10

Published: April 9, 2025

Nanotheranostics—where nanoscale materials serve both diagnostic and therapeutic functions—are rapidly transforming gene therapy by tackling critical delivery challenges. This review explores the design engineering of various nanoparticle systems (lipid-based, polymeric, inorganic, hybrid) to enhance stability, targeting, endosomal escape genetic payloads. We discuss how real-time imaging capabilities integrated into these platforms enable precise localization controlled release genes, improving treatment efficacy while reducing off-target effects. Key strategies overcome barriers (such as proton sponge effect photothermal disruption) achieve nuclear are highlighted, along with recent advances in stimuli-responsive that facilitate spatiotemporal control expression. Clinical trials preclinical studies demonstrate expanding role nanotheranostics managing cancer, inherited disorders, cardiovascular neurological diseases. further address regulatory manufacturing hurdles must be for widespread clinical adoption nanoparticle-based therapies. By synthesizing progress ongoing challenges, this underscores transformative potential effective, targeted, image-guided delivery.

Language: Английский

Citations

0
Innovative microfluidic chip for quantitative plasma separation in POCT DOI
Cuimin Sun, Peng Dai,

Wenyun Su

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163375 - 163375

Published: May 1, 2025

Citations

0
Magnetoliposomes for nanomedicine: synthesis, characterization, and applications in drug, gene, and peptide delivery DOI
Cristian F. Rodríguez, Paula Guzmán-Sastoque,

Alan Santacruz-Belalcazar

et al.

Expert Opinion on Drug Delivery, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Magnetoliposomes represent a transformative advancement in nanomedicine by integrating magnetic nanoparticles with liposomal structures, creating multifunctional delivery platforms that overcome key limitations of conventional drug carriers. These hybrid systems enable precision targeting through external fields, controlled release via hyperthermia, and real-time theranostic capabilities, offering unprecedented spatiotemporal control over therapeutic administration. This manuscript focused primarily on studies from 2023-2025 however, few select older references were included to provide background context.This review examines the fundamental design principles Magnetoliposomes, including bilayer composition, nanoparticle integration strategies, physicochemical properties governing their biological performance. We comprehensively assess synthesis methodologies - traditional thin-film hydration advanced microfluidic approaches highlighting impact colloidal stability, encapsulation, scaling potential. Characterization techniques essential for quality regulatory approval are systematically reviewed, followed applications across oncology, gene delivery, neurology, infectious disease treatment, supported recent experimental evidence. While magnetoliposomes show remarkable versatility, clinical translation requires addressing biocompatibility concerns, manufacturing scalability, hurdles. Integration artificial intelligence, organ-on-chip technologies, personalized medicine will likely accelerate development toward reality, potentially revolutionizing treatment paradigms complex diseases tailored interventions.

Language: Английский

Citations

0
Design, Characterization, and Evaluation of Textile Systems and Coatings for Sports Use: Applications in the Design of High-Thermal Comfort Wearables DOI Creative Commons
Ian C. Orjuela-Garzón, Cristian F. Rodríguez, Juan C. Cruz

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(50), P. 49143 - 49162

Published: Nov. 28, 2024

Exposure to high temperatures during indoor and outdoor activities increases the risk of heat-related illness such as cramps, rashes, heatstroke (HS). Fatal cases HS are ten times more common than serious cardiac episodes in sporting scenarios, with untreated leading mortality rates 80%. Enhancing thermal comfort can be achieved through heat loss enclosed spaces human body, utilizing transfer mechanisms radiation, conduction, convection, evaporation, which do not require initial energy input. Among these, two primary commonly employed textile industry enhance passive cooling: radiation conduction. The approach encompasses aspects: (1) reflecting solar spectrum (SS) wavelengths (2) transmitting emitting atmospheric window (AW). Conduction involves dissipating materials a conductivity. Our study focuses on combined effect these radiative conductive approaches increase loss, an area that has been extensively studied date. Therefore, main objective this project is develop, characterize, evaluate nanocomposite polymeric system using electrospinning, incorporating graphene oxide (GO) nanosheets titanium dioxide nanoparticles (TiO

Language: Английский

Citations

2
Production and purification of outer membrane vesicles encapsulating green fluorescent protein from Escherichia coli: a step towards scalable OMV technologies DOI Creative Commons
Julian Daniel Torres-Vanegas,

Nicolas Rincon-Tellez,

Paula Guzmán-Sastoque

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 14, 2024

Outer membrane vesicles (OMVs) are spherical structures that contain a small fraction of the periplasm Gram-negative bacteria, surrounded by its outer membrane. They naturally produced and detached from bacterial surface, participate in diverse biological processes, their diameter size is range 10–300 nm. OMVs have gained interest different applications, such as development biosensors, vaccines, protein chips, encapsulation heterologous proteins peptides expressed these microorganisms. However, use applications limited due to low yields high purification costs. In this study, we green fluorescent (GFP) encapsulated into using Escherichia coli JC8031 transformed with pTRC99A-ssTorA-GFP establish production route. Results showed motility strain prevents immobilization alginate, which hampers OMVs. To address issue, zeolite-based column was used chromatographically separate smaller particles. Further experiments will be focused on standardizing at scalable level.

Language: Английский

Citations

1