Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
135(31)
Published: June 20, 2023
Abstract
MicroRNAs
(miRNAs)
have
emerged
as
promising
diagnostic
biomarkers
and
therapeutic
targets
in
various
diseases.
However,
there
is
currently
a
lack
of
molecular
strategies
that
can
effectively
use
disease‐associated
extracellular
miRNAs
input
signals
to
drive
functions.
Herein,
we
present
modular
programmable
miRNA‐responsive
chimeric
DNA
receptor
(miRNA‐CDR)
capable
biomarker‐driven
therapy.
By
grafting
nanodevice
on
natural
membrane
via
aptamer
anchoring,
miRNA‐CDR
sense
miRNA
levels
autonomously
induce
dimerization‐mediated
activation
the
complementary‐mediated
strand
displacement
reaction‐induced
dynamic
assembly.
The
sequence
programmability
allows
it
respond
user‐defined
with
tunable
sensitivity.
Moreover,
versatile
customizable
reprogram
desirable
signaling
output
adapting
designated
receptor,
such
MET
FGFR1.
Using
mouse
model
drug‐induced
acute
liver
injury
(DILI),
demonstrate
functionality
designer
rewiring
recognition
DILI‐elevated
miR‐122
promote
hepatocytes
for
situ
repair
function
restoration.
Our
synthetic
platform
provides
novel
device
enabling
cellular
response,
potentially
paving
way
improving
precision
cell
therapy
regenerative
medicine.
Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: Jan. 3, 2024
Abstract
Cell
and
gene
therapies
hold
tremendous
promise
for
treating
a
range
of
difficult-to-treat
diseases.
However,
concerns
over
the
safety
efficacy
require
to
be
further
addressed
in
order
realize
their
full
potential.
Synthetic
receptors,
synthetic
biology
tool
that
can
precisely
control
function
therapeutic
cells
genetic
modules,
have
been
rapidly
developed
applied
as
powerful
solution.
Delicately
designed
engineered,
they
finetune
activities,
i.e.,
regulate
production
dosed,
bioactive
payloads
by
sensing
processing
user-defined
signals
or
biomarkers.
This
review
provides
an
overview
diverse
receptor
systems
being
used
reprogram
wide
applications
biomedical
research.
With
special
focus
on
four
at
forefront,
including
chimeric
antigen
receptors
(CARs)
Notch
(synNotch)
we
address
generalized
strategies
design,
construct
improve
receptors.
Meanwhile,
also
highlight
expanding
landscape
well
current
challenges
clinical
translation.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(31)
Published: June 20, 2023
MicroRNAs
(miRNAs)
have
emerged
as
promising
diagnostic
biomarkers
and
therapeutic
targets
in
various
diseases.
However,
there
is
currently
a
lack
of
molecular
strategies
that
can
effectively
use
disease-associated
extracellular
miRNAs
input
signals
to
drive
functions.
Herein,
we
present
modular
programmable
miRNA-responsive
chimeric
DNA
receptor
(miRNA-CDR)
capable
biomarker-driven
therapy.
By
grafting
nanodevice
on
natural
membrane
via
aptamer
anchoring,
miRNA-CDR
sense
miRNA
levels
autonomously
induce
dimerization-mediated
activation
the
complementary-mediated
strand
displacement
reaction-induced
dynamic
assembly.
The
sequence
programmability
allows
it
respond
user-defined
with
tunable
sensitivity.
Moreover,
versatile
customizable
reprogram
desirable
signaling
output
adapting
designated
receptor,
such
MET
FGFR1.
Using
mouse
model
drug-induced
acute
liver
injury
(DILI),
demonstrate
functionality
designer
rewiring
recognition
DILI-elevated
miR-122
promote
hepatocytes
for
situ
repair
function
restoration.
Our
synthetic
platform
provides
novel
device
enabling
cellular
response,
potentially
paving
way
improving
precision
cell
therapy
regenerative
medicine.
Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(34)
Published: Oct. 12, 2023
Breakthroughs
in
precision
cell
surface
engineering
tools
are
supporting
the
rapid
development
of
programmable
living
assemblies
with
valuable
features
for
tackling
complex
biological
problems.
Herein,
authors
overview
most
recent
technological
advances
chemically-
and
biologically-driven
toolboxes
mammalian
surfaces
triggering
their
assembly
into
architectures.
A
particular
focus
is
given
to
technologies
enabling
biomimetic
cell-cell
social
interactions
multicellular
cell-sorting
events.
Further
advancements
modification
may
expand
currently
available
bioengineering
toolset
unlock
a
new
generation
personalized
therapeutics
clinically
relevant
biofunctionalities.
The
combination
state-of-the-art
modifications
advanced
biofabrication
envisioned
contribute
toward
generating
materials
increasing
tissue/organ-mimetic
bioactivities
therapeutic
potential.
Development,
Journal Year:
2024,
Volume and Issue:
151(1)
Published: Jan. 1, 2024
ABSTRACT
Cell-cell
interactions
are
central
to
development,
but
exploring
how
a
change
in
any
given
cell
relates
changes
the
neighbour
of
that
can
be
technically
challenging.
Here,
we
review
recent
developments
synthetic
biology
and
image
analysis
helping
overcome
this
problem.
We
highlight
opportunities
presented
by
these
advances
discuss
limitations
applying
them
developmental
model
systems.
Advanced Biology,
Journal Year:
2023,
Volume and Issue:
7(12)
Published: Aug. 10, 2023
Abstract
The
fast‐growing
pace
of
regenerative
medicine
research
has
allowed
the
development
a
range
novel
approaches
to
tissue
engineering
applications.
Until
recently,
main
points
interest
in
majority
studies
have
been
combine
different
materials
control
cellular
behavior
and
use
techniques
optimize
formation,
from
3‐D
bioprinting
situ
regeneration.
However,
with
increase
understanding
fundamentals
organization,
development,
regeneration,
also
come
realization
that
for
next
step
engineering,
higher
level
spatiotemporal
on
cell‐matrix
interactions
is
required.
It
proposed
combination
artificial
cell
could
provide
route
toward
over
complex
development.
By
equipping
cells
underlying
mechanisms
functions,
such
as
communication
mechanisms,
migration
behavior,
or
coherent
depending
surrounding
matrix
properties,
they
can
be
applied
instructing
native
into
desired
differentiation
at
resolution
not
attained
traditional
materials.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Nov. 6, 2024
Organoids
are
"mini-organs"
that
self-organize
and
differentiate
from
stem
cells
under
in
vitro
3D
culture
conditions,
mimicking
the
spatial
structure
function
of
tissues
vivo.
Extracellular
vesicles
(EVs)
nanoscale
phospholipid
bilayer
secreted
by
living
cells,
rich
bioactive
molecules,
with
excellent
biocompatibility
low
immunogenicity.
Compared
to
EVs,
organoid-derived
EVs
(OEVs)
exhibit
higher
yield
enhanced
biological
functions.
possess
cell
characteristics,
OEVs
capable
delivering
active
substances,
making
both
highly
promising
for
medical
applications.
In
this
review,
we
provide
an
overview
fundamental
principles
organoids
OEVs,
discuss
their
current
applications
disease
treatment.
We
then
focus
on
differences
between
traditional
EVs.
Subsequently,
present
methods
engineering
modification
OEVs.
Finally,
critically
summarize
advantages
challenges
conclusion,
believe
a
deeper
understanding
will
innovative
solutions
complex
diseases.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(27)
Published: April 19, 2024
Abstract
Advancing
biofabrication
toward
manufacturing
living
constructs
with
well‐defined
architectures
and
increasingly
biologically
relevant
cell
densities
is
highly
desired
to
mimic
the
biofunctionality
of
native
human
tissues.
The
formulation
tissue‐like,
cell‐dense
inks
for
remains,
however,
challenging
at
various
levels
bioprinting
process.
Promising
advances
have
been
made
this
goal,
achieving
relatively
high
that
surpass
those
found
in
conventional
platforms,
pushing
current
boundaries
closer
tissue‐like
densities.
On
focus,
herein
overarching
challenges
bioprocessing
cell‐rich
into
clinically
grade
engineered
tissues
are
discussed,
as
well
most
recent
ink
formulations
their
processing
technologies
highlighted.
Additionally,
an
overview
foreseen
developments
field
provided
critically
discussed.
