Critical Reviews in Food Science and Nutrition,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 16
Published: April 24, 2024
Global
food
safety
stands
out
as
a
prominent
public
concern,
affecting
populations
worldwide.
The
recurrent
challenge
of
incidents
reveals
the
need
for
robust
inspection
framework.
In
recent
years,
integration
isothermal
nucleic
acid
amplification
with
CRISPR-Cas12a
techniques
has
emerged
promising
tool
molecular
detection
hazards,
presenting
next
generation
biosensing
detection.
This
paper
provides
comprehensive
review
current
state
research
on
synergistic
application
and
technology
in
field
safety.
innovative
combination
not
only
enriches
analytical
tools,
but
also
improving
assay
performance
such
sensitivity
specificity,
addressing
limitations
traditional
methods.
summarized
various
methodologies
by
diverse
concerns,
including
pathogenic
bacterium,
viruses,
mycotoxins,
adulteration,
genetically
modified
foods.
Each
section
elucidates
specific
strategies
employed
highlights
advantages
conferred.
Furthermore,
discussed
challenges
faced
this
context
safety,
offering
insightful
discussions
potential
solutions
future
prospects.
Journal of Medical Virology,
Journal Year:
2022,
Volume and Issue:
95(1)
Published: Dec. 8, 2022
The
global
outbreak
of
the
monkeypox
virus
(MPXV)
highlights
need
for
rapid
and
cost-effective
MPXV
detection
tools
to
effectively
monitor
control
disease.
Herein,
we
demonstrated
a
portable
CRISPR-Cas-based
system
naked-eye
MPXV.
harnesses
high
selectivity
CRISPR-Cas12
isothermal
nucleic
acid
amplification
potential
recombinase
polymerase
amplification.
It
can
detect
both
current
circulating
clade
original
clades.
We
reached
limit
(LoD)
22.4
aM
(13.5
copies/µl)
using
microtiter
plate
reader,
while
visual
LoD
is
75
(45
in
two-step
assay,
which
further
reduced
25
(15
one-pot
system.
compared
our
results
with
quantitative
chain
reaction
obtained
satisfactory
consistency.
For
clinical
application,
sensitive
precise
method
attomolar
sensitivity
sample-to-answer
time
35
min.
Small Methods,
Journal Year:
2022,
Volume and Issue:
6(10)
Published: Sept. 16, 2022
Abstract
Infectious
pathogens
cause
severe
human
illnesses
and
great
deaths
per
year
worldwide.
Rapid,
sensitive,
accurate
detection
of
is
importance
for
preventing
infectious
diseases
caused
by
optimizing
medical
healthcare
systems.
Inspired
a
microbial
defense
system
(i.e.,
CRISPR/
CRISPR‐associated
proteins
(Cas)
system,
an
adaptive
immune
protecting
microorganisms
from
being
attacked
invading
species),
many
new
biosensors
have
been
successfully
developed
widely
applied
in
the
viruses
pathogenic
bacteria.
Moreover,
advanced
nanotechnologies
also
integrated
into
these
to
improve
their
stability,
sensitivity,
accuracy.
In
this
review,
recent
advance
CRISPR/Cas
systems‐based
nano/biosensors
applications
bacteria
are
comprehensively
reviewed.
First
all,
categories
working
principles
systems
establishing
simply
introduced.
Then,
design
construction
discussed.
end,
attentions
focused
on
Impressively,
remaining
opportunities
challenges
further
development
system‐based
promising
proposed.
Small,
Journal Year:
2023,
Volume and Issue:
19(49)
Published: Aug. 23, 2023
Abstract
Digital
nucleic
acid
detection
based
on
microfluidics
technology
can
quantify
the
initial
amount
of
in
sample
with
low
equipment
requirements
and
simple
operations,
which
be
widely
used
clinical
vitro
diagnosis.
Recently,
isothermal
amplification
technologies
such
as
recombinase
polymerase
(RPA),
loop‐mediated
(LAMP),
clustered
regularly
interspaced
short
palindromic
repeats‐CRISPR
associated
proteins
(CRISPR‐Cas)
assisted
have
become
a
hot
spot
attention
state‐of‐the‐art
digital
chips
provided
powerful
tool
for
these
technologies.
Herein,
including
RPA,
LAMP,
CRISPR‐Cas
methods,
recently,
been
reviewed.
Moreover,
challenges
possible
strategies
to
address
them
are
discussed.
Finally,
future
directions
technology,
microfluidic
chip
device
manufacturing,
multiplex
detection,
one‐pot
outlined.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(9), P. 4193 - 4200
Published: Feb. 22, 2023
Accurate,
rapid,
and
sensitive
pathogenic
detections
play
an
important
role
in
food
safety.
Herein,
we
developed
a
novel
CRISPR/Cas12a
mediated
strand
displacement/hybridization
chain
reaction
(CSDHCR)
nucleic
acid
assay
for
foodborne
colorimetric
detection.
A
biotinylated
DNA
toehold
is
coupled
on
avidin
magnetic
beads
acts
as
initiator
to
trigger
the
SDHCR.
The
SDHCR
amplification
allowed
formation
of
long
hemin/G-quadruplex-based
DNAzyme
products
catalyze
TMB-H2O2
reaction.
In
presence
targets,
trans-cleavage
activity
was
activated
cleave
DNA,
resulting
failure
no
color
change.
Under
optimal
conditions,
CSDHCR
has
satisfactory
linear
detection
targets
with
regression
equation
Y
=
0.0531*X
-
0.0091
(R2
0.9903)
range
10
fM
1
nM,
limit
determined
4.54
fM.
addition,
Vibrio
vulnificus,
one
pathogen,
used
verify
practical
application
method,
it
showed
specificity
sensitivity
at
1.0
×
100
CFU/mL
coupling
recombinase
polymerase
amplification.
Our
proposed
biosensor
could
be
promising
alternative
method
ultrasensitive
visual
acids
pathogens.
