One-Step Immunoassay for the Detection of Influenza Virus Based on Screened Nucleoprotein (NP)-like Mimotopes from Fv-Antibody Library DOI
Tae‐Hun Kim, Jaeyong Jung,

Jeong Soo Sung

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Language: Английский

A one-step immunoassay based on switching peptides for diagnosis of porcine epidemic diarrhea virus (PEDV) using screened Fv-antibodies DOI
Tae‐Hun Kim, Jaeyeon Park, Jaeyong Jung

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(15), P. 3751 - 3763

Published: Jan. 1, 2024

In this study, a one-step immunoassay for porcine epidemic diarrhea virus (PEDV) based on Fv-antibodies and switching peptides was developed, the assay results of PEDV were obtained by just mixing samples without any further reaction or washing steps. The with binding affinity to spike protein screened from Fv-antibody library using receptor-binding domain (RBD) as screening probe. Screened affinities RBD antigen expressed, constants (KD) calculated be 83-142 nM. detection configured displacement fluorescence-labeled peptide. performed PEDV, limit (LOD) values estimated Ct = 39.7-36.4. Compared LOD value conventional lateral flow (Ct 33.0), showed remarkably improved PEDV. Finally, interaction between investigated docking simulations compared amino acid sequences receptors host cells, such aminopeptidase N (APN) angiotensin-converting enzyme-2 (ACE-2).

Language: Английский

Citations

4
Inactivation of Diutina catenulata Isolated from Longan Fruit Using Atmospheric Pressure Cold Plasma DBD in Argon, Air, and Argon-Air Mixture DOI
Ha An Quoc Than, Trung Thanh Nguyen, Ngan N. T. Kim

et al.

Food and Bioprocess Technology, Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Language: Английский

Citations

4
A microfluidic system for rapid enrichment and sensitive detection of E. coli based on bilayer membrane and high flux droplets DOI

Pengfei Gong,

Zehang Gao, Rui Sun

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128005 - 128005

Published: March 1, 2025

Language: Английский

Citations

0
One-step immunoassay of SARS-CoV-2 using screened Fv-antibodies and switching peptides DOI
Jaeyong Jung,

Jeong Soo Sung,

Ji-Hong Bong

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 245, P. 115834 - 115834

Published: Nov. 17, 2023

Language: Английский

Citations

9
Firefly Lantern-Inspired AIE-Enhanced Gold Nanocluster Microspheres for Ultrasensitive Detection of Foodborne Pathogenic Bacteria DOI

Qiaoli Wu,

Fuyuan Huang,

Yayun Jiang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136584 - 136584

Published: Sept. 4, 2024

Language: Английский

Citations

3
In Situ Preparation of Star-Shaped Protein–“Smart” Polymer Conjugates with pH and Thermo-Dual Responsibility for Bacterial Separation DOI

Fayi Wei,

Hongwei Zheng, Chao Gao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(24), P. 13943 - 13956

Published: May 31, 2024

To achieve effective separation and enrichment of bacteria, a novel synthetic scheme was developed to synthesize star-style boronate-functionalized copolymers with excellent hydrophilicity temperature pH responsiveness. A hydrophilic copolymer brush synthesized by combining surface-initiated atom-transfer radical polymerization amide reaction using bovine serum albumin as the core. The further modified introducing immobilizing fluorophenylboronic acids through an reaction, resulting in formation boronate affinity material BSA@poly(NIPAm-

Language: Английский

Citations

2
A novel strategy for specific sensing and inactivation of Escherichia coli: Constructing a targeted sandwich-type biosensor with multiple SERS hotspots to enhance SERS detection sensitivity and near-infrared light-triggered photothermal sterilization performance DOI
Rui Guo, Jingru Wang,

Wenshi Zhao

et al.

Talanta, Journal Year: 2023, Volume and Issue: 269, P. 125466 - 125466

Published: Nov. 23, 2023

Language: Английский

Citations

6
Inactivation of Diutina catenulata isolated from Longan fruit using atmospheric pressure cold plasma DBD in argon, air, and argon-air mixture DOI Creative Commons
Ha An Quoc Than, Thien Pham Huu, Trung Thanh Nguyen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

Abstract Cold plasma treatment, rapidly emerging as a revolutionary technology in food sterilization, has garnered significant attention due to its high efficacy, environmentally friendly nature, and potential for preserving food. In this study, the effectiveness of cold sterilize Diutina catenulata using argon (Ar), air, argon-air mixture at various exposure durations was evaluated by comparing inhibition zone area. The results indicated that generated from all input gas types have shown ability reducing bacteria density. Notably, maximum antibacterial circle area recorded when exposed flow rate 4 L/min with ratio 1:3 shortest treatment time. Moreover, scanning electron microscopy (SEM) observation revealed structural damage loss cell membrane integrity following treatment. These suggest promising dielectric barrier discharge (DBD) Ar air applications safety processing.

Language: Английский

Citations

2
Advances in miniaturized nanosensing platforms for analysis of pathogenic bacteria and viruses DOI
Abdallah M. Zeid, Islam M. Mostafa, Baohua Lou

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(19), P. 4160 - 4172

Published: Jan. 1, 2023

Integration of smart miniaturized nanosensors with artificial intelligence results in precise detection pathogenic bacteria or viruses biological samples.

Language: Английский

Citations

5
An efficient, high-throughput enrichment system for the rapid detection of E. coli at low concentrations in water DOI
Bin He, Fangzhou Zhang,

Pengfei Gong

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1338, P. 343571 - 343571

Published: Dec. 26, 2024

Language: Английский

Citations

1