Published: Jan. 1, 2024
Language: Английский
Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 12(5), P. 1168 - 1193
Published: Dec. 21, 2023
A biosensor is a device that reacts with the analyte to be analyzed, detects its concentration, and generates readable information, which plays an important role in medical diagnosis, detection of physiological indicators, disease prevention. Nanomaterials have received increasing attention fabrication improvement biosensors due their unique physicochemical optical properties. In this paper, properties nanomaterials such as size effect, electrical properties, advantages field biosensing are briefly summarized, application can effectively improve sensitivity reduce limit biosensors. The commonly used gold nanoparticles (AuNPs), carbon nanotubes (CNTs), quantum dots (QDs), graphene, magnetic nanobeads for applications also reviewed. Besides, two main types using involved construction working principles described, toxicity biocompatibility future direction nanomaterial discussed.
Language: Английский
Citations
56
Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123484 - 123484
Published: March 1, 2025
Wastewater-based epidemiological (WBE) surveillance has emerged as a crucial tool for monitoring infectious diseases within communities. However, its broader application is frequently constrained by the high costs, labor-intensive processes, and extended timeframes required sample collection, transportation, processing. Aptamer-based biosensors offer promising alternative, leveraging specific binding properties of aptamers to biomolecules on-site rapid quantification disease biomarkers in wastewater. This review systematically evaluates recent advancements aptamer-based detection key pathogens, including influenza viruses, SARS-CoV-2, norovirus, wastewater matrices. The discussion encompasses technical stability reliability signal transmission associated with these biosensors, well current challenges faced real-world implementation. Noteworthy progress been made development WBE, achieving limits low femtomolar (fM) levels buffer linear dynamic ranges extending up five orders magnitude viruses such SARS-CoV-2. Despite this progress, considerable hurdles remain be addressed before technologies can effectively deployed practical settings, especially complex environments. Key factors affecting performance include matrix interference, environmental variability, diminished both viral targets aptamer-target interactions not only highlights but also outlines potential avenues future research aimed at enhancing functionality applicability ultimately contributing more effective public health strategies.
Language: Английский
Citations
1
Biosensors, Journal Year: 2024, Volume and Issue: 14(3), P. 146 - 146
Published: March 15, 2024
The global challenges posed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have underscored critical importance of innovative and efficient control systems for addressing future pandemics. most effective way to is rapidly suppress spread virus through early detection using a rapid, accurate, easy-to-use diagnostic platform. In biosensors that use bioprobes, binding affinity molecular recognition elements (MREs) primary factor determining dynamic range sensing Furthermore, sensitivity relies mainly on bioprobe quality with sufficient functionality. This comprehensive review investigates aptamers nanobodies recently developed as advanced MREs SARS-CoV-2 therapeutic applications. These bioprobes might be integrated into organic bioelectronic materials devices, promising enhanced specificity. offers valuable insights advancing biosensing technologies infectious disease diagnosis treatment new bioprobes.
Language: Английский
Citations
6
ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(10), P. 11921 - 11930
Published: May 16, 2024
Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving efficacy early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as preferred choice for analyzing oncomarkers meeting ever-increasing demands diagnostics. Therefore, quest development highly specific sensitive immunosensors remains most sought-after to open new avenues personalized healthcare monitoring. Here, we report amplification-free nonlabeled DNA framework-coupled AuNPs@CuMOF ultrasensitive electrochemical detection carcinoembryonic antigen (CEA). Upon CEA concentration variation from 0.0001 200 ng mL–1, proposed biosensor led limit (LOD) 0.25 pg surpassing previously published biosensors that rely on methods aligns with those based amplification techniques. Furthermore, suggested immunosensor demonstrated auspiciously high specificity sensitivity detecting human serum comparable commercial ELISA within relative error falling between 6.25% 2.26%. The analytical approach, hinging synergy effect CuMOF's large surface area mechanical rigidity three-dimensional tetrahedron structured probes (3D TDNA), obviously broadens potential immunosensors, offering higher lower LOD. this work is not only an expansion CuMOF exploitation biosensing but also major milestone diverse biomarkers settings tumor diagnosis.
Language: Английский
Citations
5
Planta, Journal Year: 2025, Volume and Issue: 261(3)
Published: Feb. 20, 2025
Abstract Main conclusion Aptamer technology has significantly advanced the field of plant research, emerging as a tool for enhancing agricultural productivity, growth, and environmental monitoring. Aptamers are short nucleotide or amino acid sequences that can bind to range target molecules with high affinity selectivity. In recent years, these have piqued interest researchers across various scientific fields, including pharmaceuticals, analytical chemistry, science. Advancements in aptamer broadened horizons science, particularly areas analyte detection, pathogen targeting, protein function analysis. Despite use other bioassays molecular techniques small size, chemical stability, cost-effective synthesis aptamers make them invaluable tools unravelling complexities cells. Here, we discuss progress development nucleic peptide summarize their applications biotechnology. The principles signalling methods aptamer-based biosensors prospects biotechnological functional genomic studies, resistance, bioimaging discussed. Finally, present challenges future perspectives research also summarized.
Language: Английский
Citations
0
Sensors and Actuators A Physical, Journal Year: 2025, Volume and Issue: unknown, P. 116441 - 116441
Published: March 1, 2025
Language: Английский
Citations
0
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Biosensors serve as indispensable analytical tools in biomedical diagnostics, environmental monitoring, and personalized healthcare, offering operation simplicity, cost-effectiveness, high sensitivity, portability. Nanostructure integration has overcome traditional sensing platform limitations, particularly sensitivity response dynamics. These nanoscale materials-including nanoparticles, nanowires, nanosheets, nanotubes-leverage unique physicochemical properties such surface-to-volume ratio, quantum confinement effects, plasmonic interactions to enhance biosensor performance significantly. This review systematically analyzes recent advances nanostructure-based biosensing technologies, examining how nanomaterial engineering improves sensor selectivity, multifunctionality. Fundamental mechanisms are explored by which nanostructures electrochemical, optical, electrical performance, emphasizing low-abundance biomarkers complex biological matrices. Beyond technological innovations, practical applications evaluated across healthcare monitoring. Finally, current challenges outline future research directions, highlighting these technologies' potential addressed transform diagnostic capabilities outcomes.
Language: Английский
Citations
0
MedComm, Journal Year: 2025, Volume and Issue: 6(5)
Published: May 1, 2025
ABSTRACT Single‐stranded DNA or RNA entities referred to as aptamers exhibit a strong affinity and specificity for attaching specific targets. Owing their special properties, which include simplicity of synthesis, low immunogenicity, adaptability in targeting variety substances, these synthetic oligonucleotides have garnered lot interest. The function can be altered by combining them with complementary “antidotes,” are antisense particular aptamer sequence. Antidotes play an important role several fields specifically the corresponding section aptamer. Nevertheless, even promising capabilities, creation antidotes regulate inhibit continues relatively unexamined field, constraining secure efficient application medical environments. review explores experimental methodologies creating antidotes, systematic design strategies managing aptamer‐based therapies, therapeutic efficacy counteracting disease biomarkers. Additionally, it highlights diagnostic applications biosensing imaging, offering alternative traditional antibodies. It also investigates progress, latest innovations, potential uses aptamer–antidote combinations. Its academic value lies bridging gap between theoretical practical applications, providing researchers clinicians comprehensive resource advance solutions medicine biotechnology.
Language: Английский
Citations
0
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(28), P. 36804 - 36810
Published: July 6, 2024
Osteoarthritis (OA), a prevalent degenerative joint disease, significantly affects the well-being of afflicted individuals and compromises standard functionality human joints. The emerging biomarker, Cartilage acidic protein 1 (CRTAC1), intricately associates with OA initiation serves as prognostic indicator for trajectory toward replacement. However, existing diagnostic methods CRTAC1 are hampered by limited abundance, thus restricting precision specificity. Herein, novel approach utilizing single-walled carbon nanotube field-effect transistor (SWCNTs FET) biosensor is reported direct label-free detection CRTAC1. High-purity semiconducting films, functionalized antibodies CRTAC1, provide excellent electrical sensing properties. SWCNTs FET exhibits high sensitivity, notable reproducibility, wide linear range (1 fg/mL to 100 ng/mL) theoretical limit (LOD) 0.2 fg/mL. Moreover, capable directly detecting serum samples, showing performance in differentiating clinical samples from patients healthy populations. Comparative analysis traditional enzyme-linked immunosorbent assay (ELISA) reveals that proposed demonstrates faster speeds, higher sensitivity/accuracy, lower errors, indicating potential early diagnosis. Furthermore, has good scalability combined diagnosis measurement multiple disease markers, thereby expanding application FETs biosensing diagnostics.
Language: Английский
Citations
3
Analysis & Sensing, Journal Year: 2024, Volume and Issue: 5(1)
Published: Aug. 14, 2024
Abstract Carbon nano‐onions (CNOs) promise to improve the range of applications carbon materials for electroanalytical applications. In this review, we explore synthesis, characterization, and electrochemical CNOs. CNO‐based sensors present impressive features, including low detection limits in femtogram per milliliter range, a broad linear spanning up 7 orders magnitude, exceptional selectivity, reproducibility, stability. Synthetic methods characterization techniques CNOs were thoroughly examined, shedding light on their pivotal role biosensing technologies. Comparative analyses with other underscore CNOs′ competitive performance, either surpassing or matching many counterparts. Despite relatively recent integration applications, exhibit comparable superior results concerning carbon‐based materials. Indeed, incorporation into hybrid nanocomposites has shown promising outcomes, indicating synergistic potential future advancements Our review provides approach application field, emphasis breakthroughs last 5 years.
Language: Английский
Citations
2