Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs

Journal of Materials Science Materials in Medicine, Journal Year: 2025, Volume and Issue: 36(1)

Published: Jan. 13, 2025

Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications healthcare. In recent years, there has been increasing interest the development off-the-shelf using cryobioprinting approach. Here, bioinks incorporated with cryoprotective agents (CPAs) to allow fabrication cryopreservable tissue constructs. Although this method shown potential products, impact CPAs on viscoelastic behavior printability at cryo conditions remains unexplored. study, we have evaluated influence such as glycerol dimethyl sulfoxide (DMSO) rheological properties pre-crosslinked alginate for cryoprinting applications. DMSO-incorporated showed a reduction viscosity yield stress, while addition improved both due interactions calcium chloride used pre-crosslinking. Further, tube inversion experiments were performed identify suitable concentrations containing & CaCl2. Finally, based analysis cell recovery results, 10% was preservation constructs −80 °C viability cells within printed structures after recovery. Cell results indicate that bioink significantly compared without CPAs, confirming suitability developed combination fabricate on-demand

Language: Английский

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Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Reproduction and Fertility, Journal Year: 2022, Volume and Issue: 3(3), P. R121 - R146

Published: July 1, 2022

Biodiversity is defined as the presence of a variety living organisms on Earth that essential for human survival. However, anthropogenic activities are causing sixth mass extinction, threatening even our own species. For many animals, dwindling numbers becoming fragmented populations with low genetic diversity, long-term species viability. With extinction rates 1000-10,000 times greater than natural,

Language: Английский

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Local solvation structures govern the mixing thermodynamics of glycerol–water solutions

Chemical Science, Journal Year: 2023, Volume and Issue: 14(26), P. 7381 - 7392

Published: Jan. 1, 2023

Based on THz-spectra we quantify hydrophilic solvation and correlate with mixing enthalpy.

Language: Английский

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Harmonization and standardization of nucleus pulposus cell extraction and culture methods

JOR Spine, Journal Year: 2023, Volume and Issue: 6(1)

Published: Jan. 10, 2023

Abstract Background In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes much‐needed progress field. Here, an international group spine scientists collaborated standardize extraction expansion techniques for NP reduce variability, improve comparability between labs utilization funding resources. Methods The most applied methods extraction, expansion, re‐differentiation were identified a questionnaire research groups worldwide. from rat, rabbit, pig, dog, cow, human tissue experimentally assessed. Expansion media also investigated. Results Recommended protocols provided common species utilized culture. Conclusions This international, multilab multispecies study greater yield fewer gene expression changes by applying species‐specific pronase usage, 60–100 U/ml collagenase shorter durations. Recommendations passage number, many factors driving successful culture different addressed support harmonization, rigor, cross‐lab comparisons on

Language: Английский

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From freezing to functioning: cellular strategies of cold-adapted bacteria for surviving in extreme environments

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(7)

Published: June 28, 2024

Language: Английский

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A risk-based approach can guide safe cell line development and cell banking for scaled-up cultivated meat production

Nature Food, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

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Vitrification of 3D-MSCs encapsulated in GelMA hydrogel: Improved cryosurvival, reduced cryoprotectant concentration, and enhanced wound healing

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 296, P. 139716 - 139716

Published: Jan. 12, 2025

Language: Английский

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The impact of beauvericin on rainbow trout intestinal epithelial cells at different temperatures and dosing methods

In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

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Species-specific optimisation of cryopreservation media for goat and buffalo adipose-derived mesenchymal stem cells

Cryobiology, Journal Year: 2025, Volume and Issue: 118, P. 105211 - 105211

Published: Feb. 11, 2025

Language: Английский

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Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

Molecules, Journal Year: 2022, Volume and Issue: 27(10), P. 3254 - 3254

Published: May 19, 2022

Cryoprotective and cytoprotective agents (Cytoprotective Agents) are fundamental components of the cryopreservation process. This review presents essentials process by examining its drawbacks role in protecting cell physiology. Natural cryoprotective agents, such as antifreeze proteins, sugars natural deep eutectic systems, have been compared with synthetic ones, addressing their mechanisms action efficacy protection. The final part this article focuses melatonin, a hormonal substance antioxidant properties, emerging agent for somatic cells gametes, including ovarian tissue, spermatozoa spermatogonial stem cells.

Language: Английский

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