Artificial insemination with frozen‐thawed boar sperm

Molecular Reproduction and Development, Journal Year: 2017, Volume and Issue: 84(9), P. 802 - 813

Published: June 13, 2017

Artificial insemination with frozen-thawed semen in pigs is not a routine technique; its use restricted to specific cases, such as preservation of valuable genetic material (germplasm banks), safety strategies case natural disasters, long-distance transport sperm, and combination sex-sorting. Cryoinjuries resulting from freeze-thawing protocols are major concern regard the fertilization capacity treated which lower than that liquid-stored semen. Here, we provide an overview artificial using cryopreserved summarize factors influence cryopreservation success before, during, after freeze-thaw (i.e., sperm selection before starting process, holding time, cryoprotectants, rates freezing thawing) driving identification biomarkers predict sensitivity cryodamage. Three different techniques (conventional or intracervical; intrauterine; deep intrauterine) also discussed regards their relevance when Finally, review additives thawing media, given reports they may maintain improve quality fertilizing sperm. In sum, boar can reasonable fertility outcomes, if freezable ejaculates, additives, appropriate used.

Language: Английский

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Recent advances in bovine sperm cryopreservation techniques with a focus on sperm post‐thaw quality optimization

Reproduction in Domestic Animals, Journal Year: 2019, Volume and Issue: 54(4), P. 655 - 665

Published: Jan. 25, 2019

Contents Sperm cryopreservation facilitates the storage and transport of germplasm for its use in artificial insemination (AI) other advanced reproductive technologies. The process can damage sperm compromise functionality. Several cryobiological studies have found that physical biological factors affect survival at low temperatures during often involve integrity membrane. In this review, behaviour membrane against cooling, cold shock, ice crystal formation, oxidative stress, osmotic changes, reorganization lipid bilayer addition cryoprotective agents (CPA) is discussed. addition, phenomenon reactive oxygen species (ROS) relationship with also described. Semen techniques progressed slowly past years, current performance, measured as post‐thawed survival, not very different compared to decades. Recent advances understanding structure cell membrane, function metabolism driven new conservation systems, including lyophilization vitrification. However, none these technologies commercially available, although future appears promising.

Language: Английский

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Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

Animals, Journal Year: 2022, Volume and Issue: 12(23), P. 3271 - 3271

Published: Nov. 24, 2022

Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. has been available for over century, yet, using current methods, only around 50% spermatozoa retain their viability after cryopreservation. This loss associated damage different sperm components including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, microRNAs. To mitigate this damage, conventional strategies use chemical additives that include classical cryoprotectants such as glycerol, well antioxidants, fatty acids, sugars, amino membrane stabilizers. However, clearly protocols do not prevent all damage. may be due imperfect function antioxidants probable conversion media more toxic forms during

Language: Английский

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Trehalose in Biomedical Cryopreservation–Properties, Mechanisms, Delivery Methods, Applications, Benefits, and Problems

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(3), P. 1190 - 1204

Published: Feb. 13, 2023

Cells and tissues are the foundation of translational medicine. At present, one main technological obstacles is their preservation for long-term usage while maintaining adequate viability function. Optimized storage techniques must be developed to make them safer use in clinic. Cryopreservation most common method maintain vitality function cells tissues. But, formation ice crystals considered mechanism that could harm during freezing thawing. To reduce crystals, cryoprotective agents (CPAs) added achieve effect. However, conventional cryopreservation often needs toxic organic solvents as CPAs. As a result, cryopreserved may need go through time-consuming washing process remove CPAs further applications medicine, multiple valuable potentially lost or killed. Currently, trehalose has been researched nontoxic CPA due its ability stability cryopreservation. Nevertheless, nonpermeable CPA, lack an effective intracellular delivery become obstacle This article illustrated properties, mechanisms, methods, trehalose, summarized benefits limits summed up findings research direction biomedical

Language: Английский

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Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3379 - 3379

Published: Feb. 8, 2023

For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well humans. Nevertheless, the success varies across species, seasons, and latitudes even within same individual. With dawn progressive analytical techniques field genomics, proteomics, metabolomics, new options for more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics spermatozoa that could predict their cryotolerance before freezing process. Understanding changes biology result exposure to low temperatures may contribute development implementation appropriate measures assure high post-thaw quality. Furthermore, an early prediction or cryosensitivity lead establishment customized protocols interconnecting adequate processing procedures, techniques, cryosupplements are most feasible individual needs ejaculate.

Language: Английский

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Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 322 - 322

Published: March 6, 2024

Cryopreservation is a stressful process for sperm, as it associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create imbalance antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the LPO fragmentation frozen–thawed horse sperm relies upon activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) paraoxonase type 1 (PON1)); non-enzymatic capacity (Trolox-equivalent (TEAC), plasma ferric reducing (FRAP) cupric (CUPRAC)); oxidative stress index (OSI) their seminal (SP). Based on total motility integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups good- (GFEs) poor-(PFEs) freezability ejaculates. (global breaks) higher 0.05) PFE group than GFE group, thawing showing positive relationship SP OSI levels production. In addition, negatively correlated activity antioxidants (PON1 TEAC). The present results indicate that vary between These differences could from variations TEAC) balance oxidant components SP.

Language: Английский

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L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107429 - 107429

Published: Feb. 9, 2024

Language: Английский

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Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(7), P. 1958 - 1972

Published: Jan. 24, 2024

Language: Английский

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Sublethal sperm freezing damage: Manifestations and solutions

Theriogenology, Journal Year: 2018, Volume and Issue: 118, P. 172 - 181

Published: June 11, 2018

Language: Английский

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AMPK Function in Mammalian Spermatozoa

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(11), P. 3293 - 3293

Published: Oct. 23, 2018

AMP-activated protein kinase AMPK regulates cellular energy by controlling metabolism through the inhibition of anabolic pathways and simultaneous stimulation catabolic pathways. Given its central regulator role in cell metabolism, activity regulation have been focus relevant investigations, although only a few studies focused on function control spermatozoa’s ability to fertilize. This review summarizes known roles that identified mammalian spermatozoa. The involvement is described terms main physiological functions mature spermatozoa, particularly suitable sperm motility adapted fluctuating extracellular medium, maintenance integrity membranes, mitochondrial membrane potential. In addition, intracellular signaling leading activation spermatozoa are reviewed. We also discuss assisted reproduction techniques, during semen cryopreservation preservation (at 17 °C). Finally, we reinforce idea as key acts an essential linker/bridge between sperm’s

Language: Английский

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