Transcriptome analysis of boar spermatozoa with different freezability using RNA-Seq

Theriogenology, Journal Year: 2019, Volume and Issue: 142, P. 400 - 413

Published: Nov. 3, 2019

Language: Английский

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Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

BMC Veterinary Research, Journal Year: 2021, Volume and Issue: 17(1)

Published: March 22, 2021

Abstract Background Cryopreservation is an efficient way to store spermatozoa and closely associated with the quality of sperm after freeze-thaw process. During cycling, excessive reactive oxygen species (ROS) are produced, effects ROS on boar during cryopreservation have not been identified. Results In this study, we evaluated in different steps (extension, cooling, thawing for 30 min 240 min) or without boar-sperm antioxidant (N-acetylcysteine (NAC)). The levels, motility, plasma membrane integrity, mitochondrial activity, chromatin structure, ATP content, apoptosis were assayed. After thawing, level significantly increased, content impaired compared those at extension period cooling period. Moreover, addition N-acetyl L-cysteine (NAC) reversed these changes. Conclusion freeze-thawing their membrane, structure by producing ROS. Thus, downregulation antioxidants, especially NAC, important manufacturing frozen pig increase reproductive cells livestock propagation, as well improve application semen pigs worldwide.

Language: Английский

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Semenogelin, a coagulum macromolecule monitoring factor involved in the first step of fertilization: A prospective review

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 209, P. 951 - 962

Published: April 18, 2022

Language: Английский

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Preservation of boar semen: An update

Reproduction in Domestic Animals, Journal Year: 2018, Volume and Issue: 54(3), P. 423 - 434

Published: Dec. 8, 2018

Contents In the pork industry, artificial insemination and storage of boar semen in liquid at 17°C are routinely applied to optimize ejaculate bring about rapid genetic changes that reflected animal protein. Although results satisfactory, they below what occurs with natural mating. It is currently possible preserve slow freezing, since date there only one study on vitrification, negative applicable case implementing an intracytoplasmic sperm injection. both methods due sensitivity osmotic temperature changes, a loss quality initial sample; however, freezing has greater deleterious effects sample pregnancy rates number live births. Therefore, 1% all inseminations done frozen semen. The aim this review provide advances studies conducted preservation semen, delving more deeply into critical points each techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use additives extenders main quality.

Language: Английский

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Plasma membrane lipid composition and metabolomics analysis of Yorkshire boar sperms with high and low resistance to cryopreservation

Theriogenology, Journal Year: 2023, Volume and Issue: 206, P. 28 - 39

Published: April 27, 2023

Language: Английский

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Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 10983 - 10983

Published: July 1, 2023

Cryodamage affects the normal physiological functions and survivability of boar sperm during cryopreservation. Lysine acetylation is thought to be an important regulatory mechanism in functions. However, little known about protein its effects on cryotolerance or cryodamage sperm. In this study, characterization dynamics cryopreservation were determined using liquid chromatography-mass spectrometry (LC-MS). A total 1440 proteins identified out 4705 modified proteins, 2764 quantifiable sites elucidated. Among differentially sites, 1252 found upregulated compared 172 downregulated fresh frozen sperms. Gene ontology indicated that these are involved metabolic processes catalytic antioxidant activities, which pyruvate metabolism, phosphorylation lysine degradation. addition, present study demonstrated mRNA expressions SIRT5, IDH2, MDH2 LDHC, associated with quality parameters, after conclusion, induces deacetylation energy metabolism-related may contribute post-thawed parameters.

Language: Английский

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Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1673 - 1673

Published: Aug. 26, 2023

During cryopreservation, sperm undergoes structural and molecular changes such as ice crystal formation, DNA fragmentation, reactive oxygen species (ROS) production, leading to decreased quality after thawing. Antioxidants play a crucial role in preventing these damages, both vivo vitro. One potent antioxidant is myo-inositol, known for its protective effects on against ROS. This study aimed investigate the effect of myo-inositol cryopreserved boar semen. The semen was diluted, cooled, using BF5 extender. It then divided into five groups: control different concentrations (0.5, 1, 1.5, 2 mg/mL). post-thaw evaluation included assessments motility, viability, acrosome integrity, mitochondrial membrane potential (MMP), caspase activity, gene expression, ROS levels, apoptosis, IVF with treated Results showed that at 0.5 mg/mL improved fertilization ability. also reduced expression pro-apoptotic genes increased SMCP expression. Lower demonstrated viability apoptosis levels. In conclusion, treatment during cryopreservation quality, enhanced fertility rates

Language: Английский

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Antibiotics Versus Natural Biomolecules: The Case of In Vitro Induced Bacteriospermia by Enterococcus Faecalis in Rabbit Semen

Molecules, Journal Year: 2019, Volume and Issue: 24(23), P. 4329 - 4329

Published: Nov. 27, 2019

Male subfertility is a global issue in human reproduction as well animal reproduction. Bacterial infection and semen contamination are still widely overlooked. As the collection of ejaculates not sterile process, it necessary to add antimicrobial agents avoid possible depreciation samples. traditionally used antibiotics have been questioned because an ever-increasing bacterial resistance, natural bioactive molecules could offer alternative their antibacterial antioxidant properties. such, we decided compare effects selected biomolecules (resveratrol-RES, quercetin-QUE curcumin-CUR) with routinely biotechnologies (penicillin-PEN, gentamicin-GEN kanamycin-KAN) on rabbit sperm vitality presence Enterococcus faecalis. Changes structural integrity functional activity were monitored at 0, 2, 4 6 h. Computer-assisted analysis (CASA) was for assessment spermatozoa motility. Production reactive oxygen species (ROS) evaluated using chemiluminiscence, while mitochondrial membrane potential (ΔΨm) examined JC-1 dye. Finally, chromatin dispersion (SCD) test assess DNA fragmentation, changes help annexin V/propidium iodide. The motility revealed significant preservation following treatment GEN (p < 0.001), followed by PEN CUR 0.01). QUE most capable substance scavenge excessive ROS 0.001) maintain ΔΨm SCD assay that bacteria significantly 0.05) increased fragmentation. On other hand, all compounds readily preserved 0.05). In contrast antibiotics, maintained microbiological showed KAN 0.01) exhibited strongest against E. conclusion, provided protection deleterious structure function result faecalis contamination. Therefore, administration RES, and/or extenders combination carefully may be desirable.

Language: Английский

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Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(7), P. 1791 - 1791

Published: April 11, 2019

Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these changes relate to loss sperm function during cryopreservation remains unsolved. The present study aimed clarify this issue evaluating fresh and frozen-thawed pig spermatozoa retrieved from cauda epididymis ejaculate same boars, with clear differences cryotolerance. Spermatozoa were collected 10 healthy, sexually mature, fertile cryopreserved using a standard 0.5 mL-straw protocol. Total progressive motility, viability, mitochondria membrane potential higher fluidity reactive oxygen species generation lower (FT) epididymal than ejaculated Quantitative proteomics FT analyzed LC-ESI-MS/MS-based Sequential Window Acquisition All Theoretical Spectra approach. quantitatively altered more proteins Differential protein–protein networks highlighted set spermatozoa, directly involved mitochondrial functionality which would explain why deteriorate cryopreservation.

Language: Английский

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Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa

Theriogenology, Journal Year: 2019, Volume and Issue: 137, P. 36 - 42

Published: May 31, 2019

Language: Английский

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