Icariin improves pig sperm quality through antioxidant and antibacterial effects during liquid storage at 17 °C

Livestock Science, Journal Year: 2022, Volume and Issue: 256, P. 104827 - 104827

Published: Jan. 12, 2022

Language: Английский

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Relative content of Niemann-Pick C2 protein (NPC2) in seminal plasma, but not that of spermadhesin AQN-1, is related to boar sperm cryotolerance

Theriogenology, Journal Year: 2019, Volume and Issue: 145, P. 181 - 189

Published: Nov. 1, 2019

Language: Английский

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Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: July 16, 2021

Proteins play an important role in many reproductive functions such as sperm maturation, transit the female genital tract or sperm-oocyte interaction. However, general, little information concerning features is available case of aquatic animals. The present study aims to characterize proteome both spermatozoa and seminal plasma bottlenose dolphins ( Tursiops truncatus ) a model organism for cetaceans. Ejaculate samples were obtained from two trained housed aquarium. Spermatozoa analyzed by means proteomic analyses using LC-MS/MS, list with gene symbols corresponding each protein was submitted DAVID database. Of 419 proteins identified 303 plasma, 111 shared both. Furthermore, 70 involved processes, 39 spermatozoa, 31 plasma. five most abundant also these samples: AKAP3, ODF2, TUBB, GSTM3, ROPN1 CST11, LTF, ALB, HSP90B1, PIGR In conclusion, this provides first characterization cetacean opening way future research into new biomarkers, analysis conservation capacity possible additional applications field assisted technologies.

Language: Английский

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A comparative proteomic study of high and low semen quality seminal plasma in drakes

Poultry Science, Journal Year: 2022, Volume and Issue: 101(11), P. 102130 - 102130

Published: Aug. 12, 2022

Semen quality is the most important indicator in evaluating drake fecundity. At present, low semen has become a major factor restricting development of artificial insemination (AI) technology ducks. Numerous studies have indicated that seminal plasma proteins play crucial role quality, but mechanism regulating drakes remains unclear. Thus, objective this study was to identify associated with by comparing proteomic profile high-quality (HQS) and low-quality (LQS). Using label-free MS-based method, total 745 were identified. Of these, 55 differentially expressed (DEPs) identified (40 up-regulated 15 down-regulated). Gene Ontology (GO) analysis showed DEPs mainly enriched transmembrane transport, extracellular matrix structural constituent, transferase activity, transferring acyl groups other than amino-acyl groups, transporter integral component membrane (P < 0.05). Kyoto Encyclopedia Genes Genomes (KEGG) functional enrichment significantly apoptosis, tyrosine metabolism, glycerophospholipid sulfur metabolism pathways Moreover, through protein-protein interaction (PPI) network analysis, eight potential candidate identified, including P19140 (Alpha-enolase), R0KUV7 (Calreticulin), R0K3X3 (Solute carrier family 2, facilitated glucose member 5), R0L6V0 (Proteasome subunit beta), R0JKW0 (Cytochrome c), R0JMC5 (Tubulin alpha chain), R0LCK1 (Cathepsin C), R0JUP6 D), which could roles quality. Notably, further demonstrated key protein (Alpha-enolase) might can control expression related apoptosis pathway. This first systematically proteome exhibiting high These results provide novel insights into mechanisms drakes.

Language: Английский

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Deep-horn Artificial Insemination With Frozen Thawed Semen After re-extension in Autologous Seminal Plasma May Improve Pregnancy Rates in Jennies

Journal of Equine Veterinary Science, Journal Year: 2022, Volume and Issue: 112, P. 103932 - 103932

Published: March 10, 2022

Language: Английский

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