Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: April 15, 2024
Abstract
High-resolution
imaging
of
biomolecular
condensates
in
living
cells
is
essential
for
correlating
their
properties
to
those
observed
through
vitro
assays.
However,
such
experiments
are
limited
bacteria
due
resolution
limitations.
Here
we
present
an
experimental
framework
that
probes
the
formation,
reversibility,
and
dynamics
condensate-forming
proteins
Escherichia
coli
as
a
means
determine
nature
bacteria.
We
demonstrate
form
after
passing
threshold
concentration,
maintain
soluble
fraction,
dissolve
upon
shifts
temperature
exhibit
consistent
with
internal
rearrangement
exchange
between
condensed
fractions.
also
discover
established
marker
insoluble
protein
aggregates,
IbpA,
has
different
colocalization
patterns
bacterial
demonstrating
its
potential
applicability
reporter
differentiate
two
vivo.
Overall,
this
provides
generalizable,
accessible,
rigorous
set
probe
on
sub-micron
scale
cells.
Nature Reviews Drug Discovery,
Journal Year:
2022,
Volume and Issue:
21(11), P. 841 - 862
Published: Aug. 16, 2022
In
the
past
decade,
membraneless
assemblies
known
as
biomolecular
condensates
have
been
reported
to
play
key
roles
in
many
cellular
functions
by
compartmentalizing
specific
proteins
and
nucleic
acids
subcellular
environments
with
distinct
properties.
Furthermore,
growing
evidence
supports
view
that
often
form
phase
separation,
which
a
single-phase
system
demixes
into
two-phase
consisting
of
condensed
dilute
particular
biomolecules.
Emerging
understanding
condensate
function
normal
aberrant
states,
mechanisms
formation,
is
providing
new
insights
human
disease
revealing
novel
therapeutic
opportunities.
this
Perspective,
we
propose
such
could
enable
previously
unexplored
drug
discovery
approach
based
on
identifying
condensate-modifying
therapeutics
(c-mods),
discuss
strategies,
techniques
challenges
involved.
Signal Transduction and Targeted Therapy,
Journal Year:
2022,
Volume and Issue:
7(1)
Published: July 8, 2022
Abstract
Liquid–liquid
phase
separation
(LLPS)
is
a
novel
principle
for
explaining
the
precise
spatial
and
temporal
regulation
in
living
cells.
LLPS
compartmentalizes
proteins
nucleic
acids
into
micron-scale,
liquid-like,
membraneless
bodies
with
specific
functions,
which
were
recently
termed
biomolecular
condensates.
Biomolecular
condensates
are
executors
underlying
intracellular
spatiotemporal
coordination
of
various
biological
activities,
including
chromatin
organization,
genomic
stability,
DNA
damage
response
repair,
transcription,
signal
transduction.
Dysregulation
these
cellular
processes
key
event
initiation
and/or
evolution
cancer,
emerging
evidence
has
linked
formation
to
malignant
transformations
tumor
biology.
In
this
review,
we
comprehensively
summarize
detailed
mechanisms
condensate
biophysical
function
review
recent
major
advances
toward
elucidating
multiple
involved
cancer
cell
pathology
driven
by
aberrant
LLPS.
addition,
discuss
therapeutic
perspectives
research
most
developed
drug
candidates
targeting
modulation
that
can
be
used
combat
tumorigenesis.
