Biosensors integrated 3D organoid/organ-on-a-chip system: A real-time biomechanical, biophysical, and biochemical monitoring and characterization

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 231, P. 115285 - 115285

Published: April 7, 2023

Language: Английский

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Organ-on-chip models for infectious disease research

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(4), P. 891 - 904

Published: March 25, 2024

Language: Английский

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Organ-on-chip technology: Opportunities and challenges

Biotechnology Notes, Journal Year: 2024, Volume and Issue: 5, P. 8 - 12

Published: Jan. 1, 2024

Organ-on-chip (OOC) technology is an innovative approach that reproduces human organ structures and functions on microfluidic platforms, offering detailed insights into intricate physiological processes. This provides unique advantages over conventional in vitro vivo models thus has the potential to become new standard for biomedical research drug screening. In this mini-review, we compare OOCs with models, highlighting their differences, present several applications of research. Additionally, highlight advancements OOC technology, particularly developing multiorgan systems, discuss challenges future directions field.

Language: Английский

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Replacing Animal Testing with Stem Cell-Organoids : Advantages and Limitations

Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: 20(6), P. 1375 - 1386

Published: April 19, 2024

Various groups including animal protection organizations, medical research centers, and even federal agencies such as the U.S. Food Drug Administration, are working to minimize use in scientific experiments. This movement primarily stems from welfare ethical concerns. However, recent advances technology new studies medicine have contributed an increase experiments throughout years. With rapid testing, concerns arise issues, high cost, complex procedures, potential inaccuracies.Alternative solutions recently been investigated address problems of testing. Some these technologies related stem cell technologies, organ-on-a-chip, organoids, induced pluripotent models. The aim review is focus on methodologies, that can serve alternative testing discuss its advantages limitations, alongside regulatory considerations.Although methodologies has shortcomings, it replace Achieving this requires further cells, with societal technological benefits.

Language: Английский

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Circadian clock communication during homeostasis and ageing

Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

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Deciphering cartilage neuro-immune interactions and innervation profile through 3D engineered osteoarthritic micropathophysiological system

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101491 - 101491

Published: Jan. 13, 2025

Osteoarthritis (OA) is an inflammatory musculoskeletal disorder that results in cartilage breakdown and alterations the surrounding tissue microenvironment. Imbalances caused by inflammation catabolic processes potentiate pathological nerves blood vessels outgrowth toward damaged areas leading to pain patients. Yet, precise mechanisms nerve sprouting into aneural cartilaginous remain elusive. In this work, we aim recapitulate vitro hallmarks of OA pathophysiology, including sensory innervation profile, provide a sensitive reliable analytical tool monitor disease progression at microscale. Leveraging use patient-derived cells bioengineering cutting-edge technologies, engineered cartilage-like microtissues composed primary human chondrocytes encapsulated gelatin methacrylate hydrogel. Engineered constructs patterned inside microfluidic devices show expression markers, namely collagen type II, aggrecan, SOX-9 glycosaminoglycans. Upon pro-inflammatory triggering, using macrophage secretome, are recapitulated supported gene functional assays. To micropathological system, highly technology - EliChip™ presented quantitively assess molecular signature cytokines growth factors (interleukin 6 factor) produced from single chip. Herein, report miniaturized pathophysiological model foster neuro-immune interactions playing role cartilage-related disorders.

Language: Английский

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In vitro models for non-alcoholic fatty liver disease: Emerging platforms and their applications

iScience, Journal Year: 2021, Volume and Issue: 25(1), P. 103549 - 103549

Published: Dec. 4, 2021

Non-alcoholic fatty liver disease (NAFLD) represents a global healthcare challenge, affecting 1 in 4 adults, and death rates are predicted to rise inexorably. The progressive form of NAFLD, non-alcoholic steatohepatitis (NASH), can lead fibrosis, cirrhosis, hepatocellular carcinoma. However, no medical treatments licensed for NAFLD-NASH. Identifying efficacious therapies has been hindered by the complexity pathogenesis, paucity predictive preclinical models inadequate validation pharmacological targets humans. development clinically relevant vitro will pave way overcome these challenges. Currently, combined application emerging technologies (e.g., organ-on-a-chip/microphysiological systems) control engineering approaches promises unravel NAFLD biology deliver tractable treatment candidates. In this review, we describe advances NAFLD-NASH, recent introduction novel space, their importance drug discovery endeavors future.

Language: Английский

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Liver organ-on-chip models for toxicity studies and risk assessment

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(13), P. 2423 - 2450

Published: Jan. 1, 2022

This review provides an overview of liver organ-on-chip (OoC) technology, including the general concept, different approaches and recent advances. A particular focus is given to use OoC in toxicity studies risk assessment.

Language: Английский

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Recent advances for cancer detection and treatment by microfluidic technology, review and update

Biological Procedures Online, Journal Year: 2022, Volume and Issue: 24(1)

Published: April 28, 2022

Abstract Numerous cancer-associated deaths are owing to a lack of effective diagnostic and therapeutic approaches. Microfluidic systems for analyzing low volume samples offer precise, quick, user-friendly technique cancer diagnosis treatment. devices can detect many cancer-diagnostic factors from biological fluids also generate appropriate nanoparticles drug delivery. Thus, microfluidics may be valuable in the field due its high sensitivity, throughput, cost. In present article, we aim review recent achievements application microfluidic treatment various cancers. Although platforms not yet used clinic, they expected become main technology proving more sensitive accurate detection biomarkers strategies than common assays. lab-on-a-chip have shown remarkable potential designing novel procedures detection, therapy, disease follow-up as well development new delivery

Language: Английский

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Organ on Chip Technology to Model Cancer Growth and Metastasis

Bioengineering, Journal Year: 2022, Volume and Issue: 9(1), P. 28 - 28

Published: Jan. 11, 2022

Organ on chip (OOC) has emerged as a major technological breakthrough and distinct model system revolutionizing biomedical research drug discovery by recapitulating the crucial structural functional complexity of human organs in vitro. OOC are rapidly emerging powerful tools for oncology research. Indeed, Cancer (COC) can ideally reproduce certain key aspects tumor microenvironment (TME), such biochemical gradients niche factors, dynamic cell-cell cell-matrix interactions, complex tissue structures composed stromal cells. Here, we review state art COC models with focus microphysiological systems that host multicellular 3D engineering help elucidate biology TME cancer growth progression. Finally, some examples microengineered integrated multi-organ microdevices to study disease progression different tissues will be presented.

Language: Английский

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