Biotechnology for Biofuels and Bioproducts,
Journal Year:
2022,
Volume and Issue:
15(1)
Published: Dec. 29, 2022
During
the
kraft
process
to
obtain
cellulosic
pulp
from
wood,
most
of
lignin
is
removed
by
high-temperature
alkaline
cooking,
released
in
black
liquors
and
usually
incinerated
for
energy.
However,
lignins
are
a
valuable
source
phenolic
compounds
that
can
be
valorized
new
bio-based
products.
The
aim
this
work
develop
laccases
capable
working
under
extreme
conditions
high
temperature
pH,
typical
industrial
conversion
wood
into
fibreboard,
order
provide
extremophilic
biocatalysts
depolymerising
lignin,
enzyme-assisted
technologies
fibreboard
production.Through
systematic
enzyme
engineering,
combining
enzyme-directed
evolution
rational
design,
we
changed
optimal
pH
laccase
oxidation
phenols
acidic
basic,
enhanced
catalytic
activity
at
increased
thermal
tolerance
accumulating
up
eight
mutations
protein
sequence.
variants
show
maximum
70
°C
oxidize
10.
Their
integration
industrial-type
processes
saves
energy
chemicals.
As
pre-bleaching
stage,
enzymes
promote
bleachability
significantly
reduce
need
chlorine
dioxide
compared
application
chips
during
production,
facilitates
defibering
with
less
required.A
set
alkaliphilic
thermophilic
fungal
has
been
developed
operate
processes.
For
first
time
basidiomycete
high-redox
potential
on
lignin-derived
polymeric
Considering
current
tailor-made
constitute
step
forward
towards
turning
mills
biorefineries.
use
as
sector
expected
support
development
more
environmentally
sound
efficient
processes,
sustainable
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(5), P. 2865 - 2873
Published: Jan. 24, 2023
Nanozymes
can
imitate
the
catalytic
properties
of
natural
enzymes
while
overcoming
limitations
such
as
high
cost,
poor
robustness,
and
difficulty
in
recycling.
However,
rational
design
facile
preparation
nanozymes
are
still
demand.
Inspired
by
chemical
structure
laccase,
we
report
an
amorphous
metal-organic
coordination
nanocomposite
named
CuNAD,
which
is
composed
copper
ions
nicotinamide
adenine
dinucleotide
(NAD+)
via
a
simple
coordinating
coassembly
process.
As
single-site
nanozyme,
CuNAD
exhibits
excellent
robustness
under
extreme
conditions,
significantly
stronger
activity
for
phenolic
compounds,
4.02-fold
higher
sensitivity
epinephrine
detection
than
laccase.
Furthermore,
breaking
through
functional
constraints
also
able
to
activate
H2O2
at
neutral
pH,
benefiting
one-step
chromogenic
platform
cholesterol.
This
approach
demonstrates
potential
develop
biomimicking
may
boost
more
insights
into
structure-function
relationship
nanozymes.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(9), P. 886 - 886
Published: Sept. 17, 2023
Electrochemical
biosensors
based
on
immobilized
enzymes
are
among
the
most
popular
and
commercially
successful
biosensors.
The
literature
in
this
field
suggests
that
modification
of
electrodes
with
nanomaterials
is
an
excellent
method
for
enzyme
immobilization,
which
can
greatly
improve
stability
sensitivity
sensor.
However,
poor
stability,
weak
reproducibility,
limited
lifetime
itself
still
limit
requirements
development
electrochemical
food
production
process
monitoring.
Therefore,
constructing
sensing
technologies
remains
a
great
challenge.
This
article
outlines
construction
principles
four
generations
discusses
applications
single-enzyme
systems,
multi-enzyme
nano-enzyme
systems
developed
these
principles.
further
describes
methods
to
immobilization
by
combining
different
types
such
as
metals
their
oxides,
graphene-related
materials,
metal-organic
frameworks,
carbon
nanotubes,
conducting
polymers.
In
addition,
highlights
challenges
future
trends
biosensors,
providing
theoretical
support
perspectives
research
high-performance
chemical
Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 29, 2024
Abstract
The
applications
of
natural
laccases
are
greatly
restricted
because
their
drawbacks
like
poor
biostability,
high
costs,
and
low
recovery
efficiency.
M/NC
single
atom
nanozymes
(M/NC
SAzymes)
presenting
as
great
substitutes
due
to
superior
enzyme‐like
activity,
excellent
selectivity
stability.
In
this
work,
inspired
by
the
catalytic
active
center
enzyme,
a
biomimetic
Fe/NC
SAzyme
(Fe‐SAzyme)
with
O
2
‐Fe‐N
4
coordination
is
successfully
developed,
exhibiting
laccase‐like
activity.
Compared
counterpart,
Fe‐SAzyme
has
shown
efficiency
stability
under
wide
range
pH
(3.0–9.0),
temperature
(4–80
°C)
NaCl
strength
(0–300
m
).
Interestingly,
density
functional
theory
(DFT)
calculations
reveal
that
performance
attributed
activation
sites,
which
weakened
O─O
bonds
in
oxygen‐to‐water
oxidation
pathway.
Furthermore,
applied
for
efficient
aflatoxin
B
1
removal
based
on
its
robust
This
work
provides
strategy
rational
design
SAzymes,
proposed
mechanism
will
help
understand
environment
effect
SAzymes
processes.
Journal of Environmental Science and Health Part B,
Journal Year:
2022,
Volume and Issue:
57(2), P. 90 - 101
Published: Feb. 1, 2022
The
main
purpose
of
this
work
was
to
use
pineapple
crowns
as
substrate
for
optimizing
laccase
production
by
Trametes
versicolor
in
lab-scale
experiments.
One-factor-at-the-time
analysis
and
response
surface
methodology
were
used
optimize
production.
A
single
with
molecular
weight
45
kDa
the
protein
produced.
maximal
activity
60.73
±
1.01
U/g
obtained
7-day
cultures,
representing
a
6.7-fold
increase
compared
non-optimized
conditions.
optimized
conditions
temperature:
28
°C;
initial
moisture:
90%;
glucose:
8.38%;
yeast
extract:
2.86%.
Combining
stability,
best
using
during
long
periods
required
large-scale
processes
are
pH
4.0-5.0
temperature
40-50
°C.
Under
these
conditions,
crude
efficient
detoxifying
dye
malachite
green
KM
14.33
1.94
µM
Vmax
0.482
0.029
µM/min
0.1
units/mL.
It
can
be
concluded
that
crown
leaves
effectively
T.
producing
under
solid-state
culture
Laccase
is
an
industrially
relevant
enzyme
its
concomitant
valorization
offers
highly
interesting
perspectives.
