The new era of cardiovascular research: revolutionizing cardiovascular research with 3D models in a dish DOI Creative Commons
Yuan Yang, Hao Yang, Fedir N. Kiskin

et al.

Medical Review, Journal Year: 2024, Volume and Issue: 4(1), P. 68 - 85

Published: Feb. 1, 2024

Cardiovascular research has heavily relied on studies using patient samples and animal models. However, often miss the data from crucial early stage of cardiovascular diseases, as obtaining primary tissues at this is impracticable. Transgenic models can offer some insights into disease mechanisms, although they usually do not fully recapitulate phenotype diseases their progression. In recent years, a promising breakthrough emerged in form

Language: Английский

Biofabrication methods for reconstructing extracellular matrix mimetics DOI Creative Commons
Abdellah Aazmi, Duo Zhang,

Corrado Mazzaglia

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 31, P. 475 - 496

Published: Sept. 9, 2023

In the human body, almost all cells interact with extracellular matrices (ECMs), which have tissue and organ-specific compositions architectures. These ECMs not only function as cellular scaffolds, providing structural support, but also play a crucial role in dynamically regulating various functions. This comprehensive review delves into examination of biofabrication strategies used to develop bioactive materials that accurately mimic one or more biophysical biochemical properties ECMs. We discuss potential integration these ECM-mimics range physiological pathological vitro models, enhancing our understanding behavior organization. Lastly, we propose future research directions for context engineering organ-on-a-chip applications, offering advancements therapeutic approaches improved patient outcomes.

Language: Английский

Citations

59
Microfluidic high-throughput 3D cell culture DOI
Jihoon Ko, Dohyun Park,

Jungseub Lee

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(6), P. 453 - 469

Published: April 4, 2024

Language: Английский

Citations

22
New Insights on Genes, Gluten, and Immunopathogenesis of Celiac Disease DOI

Valérie Abadie,

Arnold Han, Bana Jabrì

et al.

Gastroenterology, Journal Year: 2024, Volume and Issue: 167(1), P. 4 - 22

Published: April 24, 2024

Language: Английский

Citations

18
An Organ‐on‐Chip Platform for Simulating Drug Metabolism Along the Gut–Liver Axis DOI Creative Commons
Mara Lucchetti, Kehinde Aina, Léa Grandmougin

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(20)

Published: March 7, 2024

The human microbiome significantly influences drug metabolism through the gut-liver axis, leading to modified responses and potential toxicity. Due complex nature of gut environment, understanding microbiome-driven impacts on these processes is limited. To address this, a multiorgan-on-a-chip (MOoC) platform that combines microbial-crosstalk (HuMiX) gut-on-chip (GoC) Dynamic42 liver-on-chip (LoC), mimicking bidirectional interconnection between liver known as introduced. This supports viability functionality intestinal cells. In proof-of-concept study, irinotecan, widely used colorectal cancer drug, imitated within MOoC. Utilizing liquid chromatography coupled tandem mass spectrometry (LC-MS/MS), irinotecan metabolites are tracked, confirming platform's ability represent along axis. Further, using authors' platform, it shown cancer-associated bacterium, Escherichia coli, modifies transformation its inactive metabolite SN-38G into toxic SN-38. serves robust tool for investigating intricate interplay microbes pharmaceuticals, offering representative alternative animal models providing novel development strategies.

Language: Английский

Citations

11
Let’s make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment DOI Creative Commons
Mobina Bayat, Javid Sadri Nahand

Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 40(1)

Published: July 29, 2024

Abstract Advancements in the CRISPR technology, a game-changer experimental research, have revolutionized various fields of life sciences and more profoundly, cancer research. Cell death pathways are among most deregulated cells considered as critical aspects development. Through decades, our knowledge mechanisms orchestrating programmed cellular has increased substantially, attributed to revolution cutting-edge technologies. The heroic appearance systems expanded available screening platform genome engineering toolbox detect mutations create precise edits. In that context, ability this system for identification targeting cell signaling result development therapy resistance is an auspicious choice transform accelerate individualized therapy. concept personalized stands on molecular characterization individual tumor its microenvironment order provide treatment with highest possible outcome minimum toxicity. This study explored potential technology precision by identifying specific pathways. It showed promise finding key components involved death, making it tool targeted However, also highlighted challenges limitations need be addressed future research fully realize treatment. Graphical abstract Current application through glance. A choosing appropriate biological model vitro (using established lines, animal derived cells, human stem or T cells), vivo models which can harbor tumor), ex (human/animal-derived organoids). B preparation gRNA library. C design screening, desired gRNAs phenotypic response. D CRISPR-Cas identified targets, Cas9 gene editing (Knockout, base editing, prime editing), RNA modulation (modulation splicing, interference), epigenomic edits interference/activation using dead (dCas9) (Bock et al. 2022b)

Language: Английский

Citations

10
Organ-on-a-chip: quo vademus? Applications and regulatory status DOI Creative Commons
Maria Mendes, Ana Sofia Morais,

A Carlos

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 249, P. 114507 - 114507

Published: Jan. 8, 2025

Organ-on-a-chip systems, also referred to as microphysiological systems (MPS), represent an advance in bioengineering microsystems designed mimic key aspects of human organ physiology and function. Drawing inspiration from the intricate hierarchical architecture body, these innovative platforms have emerged invaluable vitro tools with wide-ranging applications drug discovery development, well enhancing our understanding disease physiology. The facility replicate tissues within physiologically relevant three-dimensional multicellular environments empowers organ-on-a-chip versatility throughout different stages development process. Moreover, can be tailored specific states, facilitating investigation progression, responses, potential therapeutic interventions. In particular, they demonstrate, early-phase pre-clinical studies, safety toxicity profiles compounds. Furthermore, play a pivotal role evaluation efficacy modeling diseases. One most promising prospects technology is simulate pathophysiology subpopulations even individual patients, thereby being used personalized medicine. By mimicking physiological responses diverse patient groups, hold promise revolutionizing strategies, guiding them towards intervention unique needs each patient. This review presents status evolution microfluidic that facilitated transition cells organs recreated on chips some opportunities offered by technology. Additionally, current future perspectives challenges this still faces are discussed.

Language: Английский

Citations

1
Advances, challenges and future applications of liver organoids in experimental regenerative medicine DOI Creative Commons

Da Gong,

Jian Mo,

Mei Zhi Zhai

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 24, 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including metabolism of drugs and nutrients. After damage, can rapidly return to its original size if causative factor promptly eliminated. However, when harmful stimulus persists, liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at levels gene expression, molecular interactions, intercellular dynamics, complemented by successful animal studies. robust model carrier that closely resemble physiology are still lacking translating these theories into practice. potential regeneration central focus ongoing research. Over past decade, advent organoid technology provided improved models materials advancing research efforts. Liver represents novel vitro culture system. several years refinement, organoids now accurately replicate morphological structure, nutrient drug metabolism, secretory functions, providing disease Regenerative medicine aims or tissue functions repair replace damaged tissues, restore their structure function, stimulate tissues organs within body. possess same function as tissue, offering serve viable replacement liver, aligning with goals medicine. This review examines role

Language: Английский

Citations

1
Current applications of intestinal organoids: a review DOI Creative Commons
Tao Xiang, Jie Wang, Hui Li

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 31, 2024

In the past decade, intestinal organoid technology has paved way for reproducing tissue or organ morphogenesis during physiological processes in vitro and studying pathogenesis of various diseases. Intestinal organoids are favored drug screening due to their ability high-throughput cultivation closer resemblance patient genetic characteristics. Furthermore, as disease models, find wide applications diagnostic markers, identifying therapeutic targets, exploring epigenetic mechanisms Additionally, a transplantable cellular system, have played significant role reconstruction damaged epithelium conditions such ulcerative colitis short bowel syndrome, well material exchange metabolic function restoration. The rise interdisciplinary approaches, including organoid-on-chip technology, genome editing techniques, microfluidics, greatly accelerated development organoids. this review, VOSviewer software is used visualize hot co-cited journal keywords trends firstly. Subsequently, we summarized current modeling, screening, regenerative medicine. This will deepen our understanding further explore intestine

Language: Английский

Citations

8
Roadblocks confronting widespread dissemination and deployment of Organs on Chips DOI Creative Commons
Charles G. Alver, Emma Drabbe, Matthew Ishahak

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 15, 2024

Organ on Chip platforms hold significant promise as alternatives to animal models or traditional cell cultures, both of which poorly recapitulate human pathophysiology and level responses. Within the last 15 years, we have witnessed seminal scientific developments from academic laboratories, a flurry startups investments, genuine interest pharmaceutical industry well regulatory authorities translate these platforms. This Perspective identifies several fundamental design process features that may act roadblocks prevent widespread dissemination deployment systems, provides roadmap help position this technology in mainstream drug discovery.

Language: Английский

Citations

7
Organ-on-chip systems as a model for nanomedicine DOI

Marios Stavrou,

Ngan B. Phung, Jan Grimm

et al.

Nanoscale, Journal Year: 2023, Volume and Issue: 15(23), P. 9927 - 9940

Published: Jan. 1, 2023

Organ-on-chip systems recapitulate the form and function of biological organs in highly controlled vitro conditions have already been used for testing a variety nanoparticles. Can they unlock translational potential nanomedicine?

Language: Английский

Citations

13