FEMS Microbiology Ecology,
Journal Year:
2018,
Volume and Issue:
94(4)
Published: March 2, 2018
Wastewater
treatment
plants
(WWTPs)
collect
wastewater
from
various
sources
for
a
multi-step
process.
By
mixing
large
variety
of
bacteria
and
promoting
their
proximity,
WWTPs
constitute
potential
hotspots
the
emergence
antibiotic
resistant
bacteria.
Concerns
have
been
expressed
regarding
to
spread
resistance
genes
(ARGs)
environmental
reservoirs
human
pathogens.
We
utilized
epicPCR
(Emulsion,
Paired
Isolation
Concatenation
PCR)
detect
bacterial
hosts
ARGs
in
two
WWTPs.
identified
host
distribution
four
resistance-associated
(tetM,
int1,
qacEΔ1and
blaOXA-58)
influent
effluent.
The
these
varied
between
WWTP
effluent,
with
generally
decreasing
range
Through
16S
rRNA
gene
sequencing,
it
was
determined
that
carrying
include
both
abundant
rare
taxa.
Our
results
suggest
studied
mostly
succeed
during
Still,
there
were
instances
where
effluent
contained
groups
not
influent.
permitting
exhaustive
profiling
communities,
application
provides
new
level
precision
our
risk
estimates.
Environment International,
Journal Year:
2019,
Volume and Issue:
129, P. 208 - 220
Published: May 23, 2019
Wastewater
treatment
plants
(WWTPs)
are
a
source
and
reservoir
for
subsequent
spread
of
various
antibiotic
resistance
genes
(ARGs).
However,
little
is
known
about
the
activity
hosts
ARGs
in
WWTPs.
Here,
we
utilized
both
metagenomic
metatranscriptomic
approaches
to
comprehensively
reveal
diversity,
abundance,
expression
activated
sludge
(AS)
from
three
conventional
WWTPs
Taiwan.
Based
on
deep
sequencing
data
custom-made
ARG
database,
total
360
associated
with
24
classes
antibiotics
were
identified
AS
metagenomes,
an
abundance
range
7.06
×
10−1–1.20
10−4
copies
ARG/copy
16S
rRNA
gene.
Differential
coverage
binning
analysis
revealed
that
>22
bacterial
phyla
putative
ARGs.
Surprisingly,
genus
Mycobacterium
family
Burkholderiaceae
observed
as
multi-drug
resistant
harboring
14
50
Metatranscriptome
showed
65.8%
being
expressed,
highlighting
not
only
present,
but
also
transcriptionally
active
AS.
Remarkably,
110
annotated
plasmid-associated
displayed
close
two-fold
increased
likelihood
expressed
compared
those
found
exclusively
within
chromosomes.
Further
transcript
aminoglycoside,
sulfonamide,
tetracycline
was
mainly
contributed
by
plasmid-borne
Our
approach
allowed
us
specifically
link
their
transcripts
genetic
context,
providing
comprehensive
insight
into
prevalence,
Overall,
results
this
study
enhance
our
understanding
distribution
dissemination
WWTPs,
which
benefits
environmental
risk
assessment
management
ARB
Experimental & Molecular Medicine,
Journal Year:
2021,
Volume and Issue:
53(3), P. 301 - 309
Published: March 1, 2021
Abstract
The
concept
of
the
antibiotic
resistome
was
introduced
just
over
a
decade
ago,
and
since
then,
active
studies
have
been
conducted.
In
present
study,
we
describe
previously
established
resistome,
which
encompasses
all
types
resistance
genes
(ARGs),
important
findings
from
each
One-Health
sector
considering
this
concept,
thereby
emphasizing
significance
approach
in
understanding
ARG
transmission.
Cutting-edge
research
methodologies
are
essential
for
deciphering
complex
structure
microbiomes
humans,
animals,
environment.
Based
on
recent
achievements
multiple
sectors,
future
directions
suggested
to
improve
control
transmission:
(1)
ranking
critical
ARGs
their
hosts;
(2)
transmission
at
interfaces
sectors;
(3)
identifying
selective
pressures
affecting
emergence,
transmission,
evolution
ARGs;
(4)
elucidating
mechanisms
that
allow
an
organism
overcome
taxonomic
barriers
FEMS Microbiology Ecology,
Journal Year:
2018,
Volume and Issue:
94(4)
Published: March 2, 2018
Wastewater
treatment
plants
(WWTPs)
collect
wastewater
from
various
sources
for
a
multi-step
process.
By
mixing
large
variety
of
bacteria
and
promoting
their
proximity,
WWTPs
constitute
potential
hotspots
the
emergence
antibiotic
resistant
bacteria.
Concerns
have
been
expressed
regarding
to
spread
resistance
genes
(ARGs)
environmental
reservoirs
human
pathogens.
We
utilized
epicPCR
(Emulsion,
Paired
Isolation
Concatenation
PCR)
detect
bacterial
hosts
ARGs
in
two
WWTPs.
identified
host
distribution
four
resistance-associated
(tetM,
int1,
qacEΔ1and
blaOXA-58)
influent
effluent.
The
these
varied
between
WWTP
effluent,
with
generally
decreasing
range
Through
16S
rRNA
gene
sequencing,
it
was
determined
that
carrying
include
both
abundant
rare
taxa.
Our
results
suggest
studied
mostly
succeed
during
Still,
there
were
instances
where
effluent
contained
groups
not
influent.
permitting
exhaustive
profiling
communities,
application
provides
new
level
precision
our
risk
estimates.
