Candida albicans and Candida glabrata : global priority pathogens

Microbiology and Molecular Biology Reviews, Journal Year: 2024, Volume and Issue: 88(2)

Published: June 4, 2024

SUMMARYA significant increase in the incidence of

Language: Английский

---

Bacteria-derived short-chain fatty acids as potential regulators of fungal commensalism and pathogenesis

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(11), P. 1106 - 1118

Published: May 9, 2024

The human gastrointestinal microbiome encompasses bacteria, fungi, and viruses forming complex bionetworks which, for organismal health, must be in a state of homeostasis. An important homeostatic mechanism derives from microbial competition, which maintains the relative abundance species healthy balance. Microbes compete nutrients secrete metabolites that inhibit other microbes. Short-chain fatty acids (SCFAs) are one such class made by gut bacteria to very high levels. SCFAs metabolised microbes host cells have multiple roles regulating cell physiology. Here, we review mechanisms regulate fungal commensal Candida albicans. We discuss SCFA's ability growth, limit invasive behaviours modulate surface antigens recognised immune cells. underlying these roles: regulation gene expression, metabolism, signalling SCFA-driven post-translational protein modifications acylation, contribute changes acylome dynamics C. albicans with potentially large consequences Given mycobiome is reservoir systemic disease has also been implicated inflammatory bowel disease, understanding bacterial metabolites, as SCFAs, control might provide therapeutic avenues.

Language: Английский

---

Gut microbiota and metabolites as predictors of biologics response in inflammatory bowel disease: A comprehensive systematic review

Microbiological Research, Journal Year: 2024, Volume and Issue: 282, P. 127660 - 127660

Published: Feb. 28, 2024

Language: Английский

---

Controlling Candida : immune regulation of commensal fungi in the gut

Infection and Immunity, Journal Year: 2024, Volume and Issue: 92(9)

Published: April 22, 2024

ABSTRACT The intestinal microbiome harbors fungi that pose a significant risk to human health as opportunistic pathogens and drivers of inflammation. Inflammatory autoimmune diseases are associated with dysbiotic fungal communities the expansion potentially pathogenic fungi. gut is also main reservoir for disseminated infections. Immune interactions critical preventing commensal from becoming pathogenic. Significant strides have been made in defining innate adaptive immune pathways regulate fungi, these discoveries coincided advancements our understanding molecular effectors involved both colonization pathogenesis within gut. In this review, we will discuss important regulating focus on how specific cell types interact limit their or potential. This include target orchestrate antifungal responses, addition secreted effectors, such mucus antimicrobial peptides, inhibit These be framed around current niche. Finally, highlight unexplored mechanisms by which system regulates

Language: Английский

---

A fungal metabolic regulator underlies infectious synergism during Candida albicans-Staphylococcus aureus intra-abdominal co-infection

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 9, 2024

Candida albicans and Staphylococcus aureus are two commonly associated pathogens that cause nosocomial infections with high morbidity mortality. Our prior current work using a murine model of polymicrobial intra-abdominal infection (IAI) demonstrates synergistic lethality is driven by Candida-induced upregulation functional S. α-toxin leading to sepsis organ damage. In order determine the candidal effector(s) mediating enhanced virulence, an unbiased screen C. transcription factor mutants was undertaken revealing zcf13Δ/Δ fails drive augmented or lethal synergism during co-infection. A combination transcriptional phenotypic profiling approaches shows ZCF13 regulates genes involved in pentose metabolism, including RBK1 HGT7 contribute fungal ribose catabolism uptake, respectively. Subsequent experiments reveal inhibits staphylococcal agr quorum sensing system concomitantly represses toxicity. Unlike wild-type albicans, did not effectively utilize co-culture co-infection exogenous accumulation repression. Forced expression mutant fully restores pathogenicity Collectively, our results detail interwoven complexities cross-kingdom interactions highlight how intermicrobial metabolism impacts disease pathogenesis devastating consequences for host.

Language: Английский

---

Extracellular vesicles of Candida albicans show dual effects on Enterococcus faecalis growth and virulence: A laboratory‐based investigation

International Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Abstract Aim Previous studies have shown the important relationships between Enterococcus faecalis and Candida albicans in post‐treatment endodontic disease (PTED). However, fungal–bacterial interactions their possible functional routes are less understood. In this study, we investigated effect of extracellular vesicles (EVs) derived from C. on E. growth pathogenicity. Methodology EVs were isolated a yeast nitrogen base (YNB) medium, morphology, size distribution, protein concentration observed identified. The effects planktonic evaluated using curves colony‐forming unit counts, whereas biofilms determined scanning electron confocal laser microscopes. ability to resist detrimental environment, infect dentinal tubules, biofilm formation gutta percha was examined. Additionally, cell invasion cytotoxicity assessed. Statistical analysis performed one‐way variance, p ‐values <.05 considered significantly different. Results nanoparticles with bilayer membranes peak sizes 111.9 230 nm. exhibited complex its biofilms; 5 μg/mL showed inhibitory 0.156 facilitated growth. consistent virulence. Notably, reduced damage RAW264.7 cells caused by , as well macrophages intracellular survival after macrophage phagocytosis, had completely opposite effects. Conclusion dual virulence vitro suggesting involved communication. Moreover, may suggest new agent for control .

Language: Английский

---

Who arrived first? Priority effects on Candida albicans and Pseudomonas aeruginosa dual biofilms

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 3, 2025

Historical processes in community assembly, such as species arrival order, influence interactions, causing priority effects. Candida albicans and Pseudomonas aeruginosa often co-occur biofilm-based infections of the skin, lungs, medical devices. Their predominantly antagonistic relationship involves complex physical chemical interactions. However, presence implications effects among these microorganisms remain largely unexplored. Here, we investigate impact effect dual-species biofilms using clinical isolates. By varying inoculation observe significant changes biofilm composition, structure, virulence, antimicrobial susceptibility. The first colonizer has an advantage for surface colonization. Consecutive colonization increases virulence negates C. albicans' protective on P. PAET1 against meropenem treatment. Finally, propose N-acetylcysteine adjuvant treating interkingdom infections, working independently Polymicrobial demonstrated that effects, influenced by species' affect

Language: Английский

---

Commensalism and pathogenesis of Candida albicans at the mucosal interface

Nature Reviews Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

---

Overview of Microorganisms: Bacterial Microbiome, Mycobiome, Virome Identified Using Next-Generation Sequencing, and Their Application to Ophthalmic Diseases

Microorganisms, Journal Year: 2025, Volume and Issue: 13(6), P. 1300 - 1300

Published: June 3, 2025

This review outlines technological advances in pathogen identification and describes the development evolution of next-generation sequencers that can be applied to ocular microbiome. Traditional methods such as culture PCR have limitations detecting full spectrum resident microorganisms, prompting a transition toward metagenomic analysis. As microbiome research expands across body systems, comprehensive bacteria, fungi, viruses has become possible. The commensal may influence disease through changes immune system environment. Next-generation sequencing enables detailed microbial profiling, aiding diagnosis treatment selection. Alterations also induce metabolic changes, offering insights into novel methods. technology, summarizes current knowledge microorganisms found on surface intraocular fluid, discusses future challenges prospects. However, large volume data obtained must interpreted with caution due possible analytical biases. Furthermore, determining whether is truly pathogenic requires interpretation beyond clinical findings results traditional

Language: Английский

---

Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation

Microbiology, Journal Year: 2024, Volume and Issue: 170(1)

Published: Jan. 23, 2024

Polymicrobial infection with Candida albicans and Staphylococcus aureus may result in a concomitant increase virulence resistance to antimicrobial drugs. This enhanced pathogenicity phenotype is mediated by numerous factors, including metabolic processes direct interaction of S. C. hyphae. The overall structure biofilms known contribute their recalcitrance treatment, although the dynamics between species how it contributes poorly understood. To address this, novel time-lapse mesoscopic optical imaging method was developed enable formation / whole dual-species be followed. It found that yeast-form or hyphal-form biofilm founder population profoundly affects as matures. Different sub-populations arise within each different morphotypes, resulting distinct sub-regions. These data reveal cell morphology pivotal development global architecture emergence colony macrostructures temporally influence synergy infection.

Language: Английский

---