Phenotypic landscape of a fungal meningitis pathogen reveals its unique biology DOI Creative Commons
Michael J. Boucher, Sanjita Banerjee,

Meenakshi Joshi

et al.

Published: Oct. 22, 2024

Cryptococcus neoformans is the most common cause of fungal meningitis and top-ranked W.H.O. priority pathogen. Only distantly related to model fungi, C. also a powerful experimental system for exploring conserved eukaryotic mechanisms lost from specialist yeast lineages. To decipher its biology globally, we constructed 4328 gene deletions measured--with exceptional precision--the fitness each mutant under 141 diverse growth-limiting in vitro conditions during murine infection. We defined functional modules by clustering genes based on their phenotypic signatures. In-depth studies leveraged these data two ways. First, investigated new components key signaling pathways, which revealed animal-like pathways/components not predicted yeasts. Second, identified environmental adaptation repurposed promote mammalian virulence neoformans, lacks known animal reservoir. Our work provides an unprecedented resource deciphering deadly human

Language: Английский

A human commensal-pathogenic fungus suppresses host immunity via targeting TBK1 DOI
Gang Luo, Jingkai Zhang, Tianxu Wang

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(9), P. 1536 - 1551.e6

Published: July 30, 2024

Language: Английский

Citations

3
Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation DOI
Camila Figueiredo Pinzan, Clara Valero, Patrícia Alves de Castro

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(10), P. 2710 - 2726

Published: Aug. 27, 2024

Language: Английский

Citations

3
Evolutionary insights and functional diversity of gasdermin family proteins and homologs in microorganisms DOI Creative Commons
Shule Wang,

Tingbo Ma,

Xiaoyi Xia

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

The gasdermin protein family and its homologs in microorganisms have gained significant attention due to their roles programmed cell death, immune defense, microbial infection. This review summarizes the current research status of proteins, structural features, functional fungi, bacteria, viruses. presents evolutionary parallels between mammalian defense systems, highlighting conserved role proteins regulating death processes immunity. Additionally, characteristics are summarized, shedding light on potential as targets for therapeutic interventions. Future directions this field also discussed provide a roadmap further investigation.

Language: Английский

Citations

1
Phenotypic landscape of a fungal meningitis pathogen reveals its unique biology DOI Creative Commons
Michael J. Boucher, Sanjita Banerjee,

Meenakshi Joshi

et al.

Published: Oct. 22, 2024

Cryptococcus neoformans is the most common cause of fungal meningitis and top-ranked W.H.O. priority pathogen. Only distantly related to model fungi, C. also a powerful experimental system for exploring conserved eukaryotic mechanisms lost from specialist yeast lineages. To decipher its biology globally, we constructed 4328 gene deletions measured--with exceptional precision--the fitness each mutant under 141 diverse growth-limiting in vitro conditions during murine infection. We defined functional modules by clustering genes based on their phenotypic signatures. In-depth studies leveraged these data two ways. First, investigated new components key signaling pathways, which revealed animal-like pathways/components not predicted yeasts. Second, identified environmental adaptation repurposed promote mammalian virulence neoformans, lacks known animal reservoir. Our work provides an unprecedented resource deciphering deadly human

Language: Английский

Citations

1